Gene/Protein Characteristic Table for KIAA0089
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Product ID ORK00011
Accession No D42047
Description glycerol-3-phosphate dehydrogenase 1-like
Clone name ha03662
Vector information
The cDNA fragment was inserted at the EcoRV-NotI site of the ...
cDNA sequence DNA sequence (4043 bp)
Predicted protein sequence (411 aa)
Flexi ORF Clone FXC00011
Source Myeloblast cell line (KG-1)
Rouge ID mKIAA0089 by Kazusa Mouse cDNA Project
Features of the cloned cDNA sequence
Description

Length: 4043 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq Warning No warning
Integrity of 3' end
Length of 3'UTR 2806 bp
Genome contig ID gi89161205f_32023027
PolyA signal sequence
(AATAAA,-29)
+----*----+----*----+----*----+----
TGATTTAATAAACATTTAACCCCCTAAACTTGGGG
Flanking genome sequence
(162184 - 162233)
----+----*----+----*----+----*----+----*----+----*
CACCGTGGATCCTGTTTTTCTTTTTATCAGCTTAGGTAATCATTTTATTT

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 3 f 32123027 32185209 8 99.2 Perfect prediction
Features of the protein sequence
Description

Length: 411 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
XP_001096149 3.3e-143 94.3 similar to glyc...
Macaca mulatta
Q8N335 2.2e-140 100.0 Glycerol-3-phos...
Homo sapiens
Q5R5V3 1.8e-139 99.4 Glycerol-3-phos...
Pongo abelii
2PLA 2e-139 100.0 Crystal structu...
Homo sapiens
AAI49967 7e-137 97.4 GPD1L protein [...
Bos taurus
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
No significant homologues
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
BlastProDom IPR006109 263 408 PD001278 NAD-dependent glycerol-3-phosphate dehydrogenase
FPrintScan IPR006168 70 87 PR00077 NAD-dependent glycerol-3-phosphate dehydrogenase
IPR006168 132 159 PR00077 NAD-dependent glycerol-3-phosphate dehydrogenase
IPR006168 208 228 PR00077 NAD-dependent glycerol-3-phosphate dehydrogenase
IPR006168 248 272 PR00077 NAD-dependent glycerol-3-phosphate dehydrogenase
IPR006168 273 297 PR00077 NAD-dependent glycerol-3-phosphate dehydrogenase
IPR006168 316 333 PR00077 NAD-dependent glycerol-3-phosphate dehydrogenase
HMMPfam IPR011128 66 237 PF01210 NAD-dependent glycerol-3-phosphate dehydrogenase
IPR006109 254 404 PF07479 NAD-dependent glycerol-3-phosphate dehydrogenase
Expression profile
Description

Northern blot
Experimental conditions
Primer_f
Primer_r
PCR conditions test

RH mapping information
Description

Chromosome No. 3
Experimental conditions
Panel name Stanford G3
Primer_f ACTGAAGAGATTTTGGTGAGG
Primer_r TCAGAACCAACAATGCCACAG
PCR product length 146 bp
PCR conditions 95 °C15 sec62 °C120 sec30 cycles
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