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[1][TOP]
>UniRef100_Q9SSF4 MLP3.1 protein (Fragment) n=1 Tax=Arabidopsis thaliana
RepID=Q9SSF4_ARATH
Length = 197
Score = 91.3 bits (225), Expect = 4e-17
Identities = 41/57 (71%), Positives = 48/57 (84%)
Frame = -1
Query: 538 SAFQIQENINLFCQTRDNIFKIMNDLCDTPEVMKQMPPLPVKVNDELANSILPRTHH 368
SAFQ+ EN+N+ C+ RDNI I+NDL D PEVMKQMPPLPVK+N+ELANSILPR H
Sbjct: 137 SAFQLHENVNILCKARDNILAILNDLNDMPEVMKQMPPLPVKLNEELANSILPRPSH 193
[2][TOP]
>UniRef100_Q8RXW7 Putative uncharacterized protein At3g07565 (Fragment) n=1
Tax=Arabidopsis thaliana RepID=Q8RXW7_ARATH
Length = 74
Score = 91.3 bits (225), Expect = 4e-17
Identities = 41/57 (71%), Positives = 48/57 (84%)
Frame = -1
Query: 538 SAFQIQENINLFCQTRDNIFKIMNDLCDTPEVMKQMPPLPVKVNDELANSILPRTHH 368
SAFQ+ EN+N+ C+ RDNI I+NDL D PEVMKQMPPLPVK+N+ELANSILPR H
Sbjct: 14 SAFQLHENVNILCKARDNILAILNDLNDMPEVMKQMPPLPVKLNEELANSILPRPSH 70
[3][TOP]
>UniRef100_Q84WV3 Putative uncharacterized protein At3g07565 n=1 Tax=Arabidopsis
thaliana RepID=Q84WV3_ARATH
Length = 258
Score = 91.3 bits (225), Expect = 4e-17
Identities = 41/57 (71%), Positives = 48/57 (84%)
Frame = -1
Query: 538 SAFQIQENINLFCQTRDNIFKIMNDLCDTPEVMKQMPPLPVKVNDELANSILPRTHH 368
SAFQ+ EN+N+ C+ RDNI I+NDL D PEVMKQMPPLPVK+N+ELANSILPR H
Sbjct: 198 SAFQLHENVNILCKARDNILAILNDLNDMPEVMKQMPPLPVKLNEELANSILPRPSH 254
[4][TOP]
>UniRef100_Q2V3X6 Putative uncharacterized protein At3g07565.3 n=1 Tax=Arabidopsis
thaliana RepID=Q2V3X6_ARATH
Length = 259
Score = 91.3 bits (225), Expect = 4e-17
Identities = 41/57 (71%), Positives = 48/57 (84%)
Frame = -1
Query: 538 SAFQIQENINLFCQTRDNIFKIMNDLCDTPEVMKQMPPLPVKVNDELANSILPRTHH 368
SAFQ+ EN+N+ C+ RDNI I+NDL D PEVMKQMPPLPVK+N+ELANSILPR H
Sbjct: 199 SAFQLHENVNILCKARDNILAILNDLNDMPEVMKQMPPLPVKLNEELANSILPRPSH 255
[5][TOP]
>UniRef100_A7QAL5 Chromosome chr5 scaffold_72, whole genome shotgun sequence n=1
Tax=Vitis vinifera RepID=A7QAL5_VITVI
Length = 254
Score = 88.6 bits (218), Expect = 3e-16
Identities = 40/58 (68%), Positives = 49/58 (84%)
Frame = -1
Query: 541 LSAFQIQENINLFCQTRDNIFKIMNDLCDTPEVMKQMPPLPVKVNDELANSILPRTHH 368
L++ QIQ+NI+LFCQ RDNI I+NDL D PEVM+QMPPLPVK+N++L NSILPR H
Sbjct: 190 LASLQIQDNISLFCQARDNIQAILNDLNDMPEVMRQMPPLPVKINEDLVNSILPRAAH 247
[6][TOP]
>UniRef100_B9SW75 Putative uncharacterized protein n=1 Tax=Ricinus communis
RepID=B9SW75_RICCO
Length = 250
Score = 87.0 bits (214), Expect = 8e-16
Identities = 39/55 (70%), Positives = 48/55 (87%)
Frame = -1
Query: 541 LSAFQIQENINLFCQTRDNIFKIMNDLCDTPEVMKQMPPLPVKVNDELANSILPR 377
LS Q+QENI L CQTRDNI KIMN++ D PE+MK++PPLPVK+N+ELAN+ILPR
Sbjct: 190 LSTMQLQENIGLLCQTRDNILKIMNEMKDMPELMKRLPPLPVKLNEELANAILPR 244
[7][TOP]
>UniRef100_Q01IQ4 OSIGBa0159F11.10 protein n=3 Tax=Oryza sativa RepID=Q01IQ4_ORYSA
Length = 246
Score = 84.0 bits (206), Expect = 6e-15
Identities = 35/55 (63%), Positives = 47/55 (85%)
Frame = -1
Query: 541 LSAFQIQENINLFCQTRDNIFKIMNDLCDTPEVMKQMPPLPVKVNDELANSILPR 377
LS QIQ+NI+L CQTRDNI +++ ++ D P++MKQMPPLPVK+N+EL NS+LPR
Sbjct: 186 LSTMQIQDNISLLCQTRDNILRVLKEINDAPDIMKQMPPLPVKINEELVNSMLPR 240
[8][TOP]
>UniRef100_C5YBG1 Putative uncharacterized protein Sb06g021360 n=1 Tax=Sorghum
bicolor RepID=C5YBG1_SORBI
Length = 229
Score = 83.6 bits (205), Expect = 8e-15
Identities = 35/55 (63%), Positives = 47/55 (85%)
Frame = -1
Query: 541 LSAFQIQENINLFCQTRDNIFKIMNDLCDTPEVMKQMPPLPVKVNDELANSILPR 377
LS QIQ+NI+L CQTRDNI +++ ++ D PE+MKQMPPLPVK+N++L NS+LPR
Sbjct: 169 LSNMQIQDNISLLCQTRDNILRVLKEINDAPEIMKQMPPLPVKINEDLVNSLLPR 223
[9][TOP]
>UniRef100_B4FB11 Myb-like DNA-binding domain containing protein n=1 Tax=Zea mays
RepID=B4FB11_MAIZE
Length = 241
Score = 83.6 bits (205), Expect = 8e-15
Identities = 35/55 (63%), Positives = 47/55 (85%)
Frame = -1
Query: 541 LSAFQIQENINLFCQTRDNIFKIMNDLCDTPEVMKQMPPLPVKVNDELANSILPR 377
LS QIQ+NI+L CQTRDNI +++ ++ D P++MKQMPPLPVK+N+EL NS+LPR
Sbjct: 181 LSNMQIQDNISLLCQTRDNILRVLKEINDAPDIMKQMPPLPVKINEELVNSLLPR 235
[10][TOP]
>UniRef100_Q0DJA9 Os05g0305700 protein n=2 Tax=Oryza sativa RepID=Q0DJA9_ORYSJ
Length = 235
Score = 83.6 bits (205), Expect = 8e-15
Identities = 36/58 (62%), Positives = 47/58 (81%)
Frame = -1
Query: 541 LSAFQIQENINLFCQTRDNIFKIMNDLCDTPEVMKQMPPLPVKVNDELANSILPRTHH 368
+S Q+QENI L CQTRDNI ++ ++ D PE+M+QMPPLPVK+N+ELANS+LPR H
Sbjct: 177 ISNMQVQENIPLLCQTRDNILAVLKEIGDVPEIMRQMPPLPVKLNEELANSMLPRPPH 234
[11][TOP]
>UniRef100_C6TNY1 Putative uncharacterized protein n=1 Tax=Glycine max
RepID=C6TNY1_SOYBN
Length = 232
Score = 80.9 bits (198), Expect = 5e-14
Identities = 37/59 (62%), Positives = 46/59 (77%)
Frame = -1
Query: 541 LSAFQIQENINLFCQTRDNIFKIMNDLCDTPEVMKQMPPLPVKVNDELANSILPRTHHH 365
L+A Q+Q NINLF QTRDNI KI+ND+ D E MKQMPPLP K+N+ + NSIL +T+ H
Sbjct: 172 LAALQLQGNINLFSQTRDNIIKILNDMNDMQEAMKQMPPLPFKINETIFNSILSKTNQH 230
[12][TOP]
>UniRef100_B9HGY3 Predicted protein (Fragment) n=1 Tax=Populus trichocarpa
RepID=B9HGY3_POPTR
Length = 218
Score = 79.7 bits (195), Expect = 1e-13
Identities = 37/53 (69%), Positives = 47/53 (88%)
Frame = -1
Query: 541 LSAFQIQENINLFCQTRDNIFKIMNDLCDTPEVMKQMPPLPVKVNDELANSIL 383
L++FQIQEN+NL +TRDNI KIMN + D PE+MKQMPPLPVK+ND+LA++IL
Sbjct: 159 LASFQIQENLNLLRRTRDNIRKIMNQMNDVPELMKQMPPLPVKLNDDLADTIL 211
[13][TOP]
>UniRef100_B9MY93 Predicted protein n=1 Tax=Populus trichocarpa RepID=B9MY93_POPTR
Length = 240
Score = 79.0 bits (193), Expect = 2e-13
Identities = 35/53 (66%), Positives = 48/53 (90%)
Frame = -1
Query: 541 LSAFQIQENINLFCQTRDNIFKIMNDLCDTPEVMKQMPPLPVKVNDELANSIL 383
L+++QIQEN++L QTRDNI K+MN++ D PE+MKQMPPLPVK+ND+LA++IL
Sbjct: 180 LASYQIQENLSLLRQTRDNICKVMNEMDDVPELMKQMPPLPVKLNDDLADTIL 232
[14][TOP]
>UniRef100_B9IAZ2 Predicted protein (Fragment) n=1 Tax=Populus trichocarpa
RepID=B9IAZ2_POPTR
Length = 198
Score = 77.4 bits (189), Expect = 6e-13
Identities = 33/53 (62%), Positives = 45/53 (84%)
Frame = -1
Query: 538 SAFQIQENINLFCQTRDNIFKIMNDLCDTPEVMKQMPPLPVKVNDELANSILP 380
S+ Q+ +NINL C++R+NI ++NDL D PE MKQMPPLPVK+N+ELA++ILP
Sbjct: 146 SSIQVHDNINLLCKSRENILALLNDLNDMPETMKQMPPLPVKLNEELASNILP 198
[15][TOP]
>UniRef100_B9RY47 Putative uncharacterized protein n=1 Tax=Ricinus communis
RepID=B9RY47_RICCO
Length = 304
Score = 77.0 bits (188), Expect = 8e-13
Identities = 34/56 (60%), Positives = 45/56 (80%)
Frame = -1
Query: 541 LSAFQIQENINLFCQTRDNIFKIMNDLCDTPEVMKQMPPLPVKVNDELANSILPRT 374
LS F++Q+NI+LFC+TR NI I+ND+ P +M QMPPLPV +N++LANSILP T
Sbjct: 229 LSTFKLQDNIDLFCRTRTNITAILNDMRGMPGIMSQMPPLPVSINEDLANSILPST 284
[16][TOP]
>UniRef100_UPI00019829F0 PREDICTED: hypothetical protein n=1 Tax=Vitis vinifera
RepID=UPI00019829F0
Length = 310
Score = 76.6 bits (187), Expect = 1e-12
Identities = 34/56 (60%), Positives = 45/56 (80%)
Frame = -1
Query: 541 LSAFQIQENINLFCQTRDNIFKIMNDLCDTPEVMKQMPPLPVKVNDELANSILPRT 374
LS F++Q+NI+LFC+TRDNI I+ND+ D P +M MPPL V +N++LANSILP T
Sbjct: 235 LSTFKLQDNIDLFCRTRDNITAILNDMRDMPGIMSHMPPLNVYINEDLANSILPNT 290
[17][TOP]
>UniRef100_A7PNH7 Chromosome chr1 scaffold_22, whole genome shotgun sequence n=1
Tax=Vitis vinifera RepID=A7PNH7_VITVI
Length = 263
Score = 76.6 bits (187), Expect = 1e-12
Identities = 34/56 (60%), Positives = 45/56 (80%)
Frame = -1
Query: 541 LSAFQIQENINLFCQTRDNIFKIMNDLCDTPEVMKQMPPLPVKVNDELANSILPRT 374
LS F++Q+NI+LFC+TRDNI I+ND+ D P +M MPPL V +N++LANSILP T
Sbjct: 188 LSTFKLQDNIDLFCRTRDNITAILNDMRDMPGIMSHMPPLNVYINEDLANSILPNT 243
[18][TOP]
>UniRef100_B9HJQ7 Predicted protein n=1 Tax=Populus trichocarpa RepID=B9HJQ7_POPTR
Length = 256
Score = 73.6 bits (179), Expect = 9e-12
Identities = 31/56 (55%), Positives = 45/56 (80%)
Frame = -1
Query: 541 LSAFQIQENINLFCQTRDNIFKIMNDLCDTPEVMKQMPPLPVKVNDELANSILPRT 374
LS F++Q+NI+ FC TR+NI I+ND+ + P +M +MPPLPV ++++LANSILP T
Sbjct: 181 LSTFKLQDNIDFFCYTRNNITAILNDMREMPGIMSRMPPLPVSIDEDLANSILPNT 236
[19][TOP]
>UniRef100_B8LQS9 Putative uncharacterized protein n=1 Tax=Picea sitchensis
RepID=B8LQS9_PICSI
Length = 149
Score = 73.6 bits (179), Expect = 9e-12
Identities = 31/54 (57%), Positives = 46/54 (85%)
Frame = -1
Query: 541 LSAFQIQENINLFCQTRDNIFKIMNDLCDTPEVMKQMPPLPVKVNDELANSILP 380
L++++IQEN+NL C+TR+NI I+ D+ D P +M QMPPLPV++N++LA+SILP
Sbjct: 88 LASYRIQENMNLLCRTRNNIVAILKDMKDMPGIMSQMPPLPVRLNEDLADSILP 141
[20][TOP]
>UniRef100_UPI00019833BE PREDICTED: hypothetical protein n=1 Tax=Vitis vinifera
RepID=UPI00019833BE
Length = 279
Score = 70.5 bits (171), Expect = 7e-11
Identities = 31/52 (59%), Positives = 42/52 (80%)
Frame = -1
Query: 538 SAFQIQENINLFCQTRDNIFKIMNDLCDTPEVMKQMPPLPVKVNDELANSIL 383
S+ QI++NI+LFCQ RDN+ ++N L D PE MK+MPPLP K+N+E+ NSIL
Sbjct: 217 SSLQIEDNISLFCQARDNLQVLLNSLKDEPEAMKKMPPLPDKLNEEMINSIL 268
[21][TOP]
>UniRef100_A9TT52 Predicted protein n=1 Tax=Physcomitrella patens subsp. patens
RepID=A9TT52_PHYPA
Length = 231
Score = 69.3 bits (168), Expect = 2e-10
Identities = 34/63 (53%), Positives = 43/63 (68%)
Frame = -1
Query: 541 LSAFQIQENINLFCQTRDNIFKIMNDLCDTPEVMKQMPPLPVKVNDELANSILPRTHHHH 362
L+A Q QEN +L Q RDNI+ I+N + P +M QMPPLPVK+N ELA+SILP+
Sbjct: 169 LAASQAQENTDLLVQVRDNIYAILNGMMSMPGIMSQMPPLPVKLNSELADSILPQPTPQP 228
Query: 361 DAA 353
AA
Sbjct: 229 SAA 231
[22][TOP]
>UniRef100_A9U030 Predicted protein n=1 Tax=Physcomitrella patens subsp. patens
RepID=A9U030_PHYPA
Length = 287
Score = 67.4 bits (163), Expect = 6e-10
Identities = 30/56 (53%), Positives = 44/56 (78%)
Frame = -1
Query: 541 LSAFQIQENINLFCQTRDNIFKIMNDLCDTPEVMKQMPPLPVKVNDELANSILPRT 374
L++ ++QEN +L + RDNIF I+N + + P +M QMPPLPVK+N ELA+SILP++
Sbjct: 222 LASMKMQENTDLLVRFRDNIFAILNGMTNMPGIMSQMPPLPVKLNTELADSILPKS 277
[23][TOP]
>UniRef100_A9SMR9 Predicted protein n=1 Tax=Physcomitrella patens subsp. patens
RepID=A9SMR9_PHYPA
Length = 290
Score = 67.4 bits (163), Expect = 6e-10
Identities = 30/56 (53%), Positives = 44/56 (78%)
Frame = -1
Query: 541 LSAFQIQENINLFCQTRDNIFKIMNDLCDTPEVMKQMPPLPVKVNDELANSILPRT 374
L++ ++QEN +L + RDNIF I+N + + P +M QMPPLPVK+N ELA+SILP++
Sbjct: 225 LASMKMQENTDLLVRFRDNIFAILNGMTNMPGIMSQMPPLPVKLNTELADSILPKS 280
[24][TOP]
>UniRef100_B9HVL6 Predicted protein n=1 Tax=Populus trichocarpa RepID=B9HVL6_POPTR
Length = 552
Score = 67.0 bits (162), Expect = 8e-10
Identities = 31/52 (59%), Positives = 41/52 (78%)
Frame = -1
Query: 529 QIQENINLFCQTRDNIFKIMNDLCDTPEVMKQMPPLPVKVNDELANSILPRT 374
Q+Q+NI+L C TR+NI I+ND+ + P M QMPPLPV +N++LANSILP T
Sbjct: 292 QLQDNIDLLCHTRNNITAILNDMREMPG-MSQMPPLPVSINEDLANSILPNT 342
[25][TOP]
>UniRef100_C6TKF2 Putative uncharacterized protein (Fragment) n=1 Tax=Glycine max
RepID=C6TKF2_SOYBN
Length = 297
Score = 65.9 bits (159), Expect = 2e-09
Identities = 28/53 (52%), Positives = 42/53 (79%)
Frame = -1
Query: 541 LSAFQIQENINLFCQTRDNIFKIMNDLCDTPEVMKQMPPLPVKVNDELANSIL 383
LS +++Q+NI+LFC TR NI I+N++ + P +M QMPPL V +N++LA+SIL
Sbjct: 225 LSTYKLQDNIDLFCHTRHNINTILNNMSEMPGIMSQMPPLQVNINEDLASSIL 277
[26][TOP]
>UniRef100_A7QAM2 Chromosome chr5 scaffold_72, whole genome shotgun sequence n=1
Tax=Vitis vinifera RepID=A7QAM2_VITVI
Length = 317
Score = 63.5 bits (153), Expect = 9e-09
Identities = 30/52 (57%), Positives = 41/52 (78%)
Frame = -1
Query: 538 SAFQIQENINLFCQTRDNIFKIMNDLCDTPEVMKQMPPLPVKVNDELANSIL 383
S+ QI++NI+LFCQ RDN+ ++N D PE MK+MPPLP K+N+E+ NSIL
Sbjct: 256 SSLQIEDNISLFCQARDNLQVLLNRH-DEPEAMKKMPPLPDKLNEEMINSIL 306
[27][TOP]
>UniRef100_Q8GX21 Putative uncharacterized protein At1g10820/T16B5_4 n=1
Tax=Arabidopsis thaliana RepID=Q8GX21_ARATH
Length = 232
Score = 60.1 bits (144), Expect = 1e-07
Identities = 27/56 (48%), Positives = 42/56 (75%)
Frame = -1
Query: 541 LSAFQIQENINLFCQTRDNIFKIMNDLCDTPEVMKQMPPLPVKVNDELANSILPRT 374
LSA ++Q+NI+LF Q R+NI I+ D+ + P +M +MP LPV +ND+LA+++L T
Sbjct: 157 LSACKLQDNISLFHQARNNISAILTDMKEMPGIMSRMPALPVSINDDLASNLLSST 212
[28][TOP]
>UniRef100_Q3EDE9 Uncharacterized protein At1g10820.2 n=1 Tax=Arabidopsis thaliana
RepID=Q3EDE9_ARATH
Length = 258
Score = 60.1 bits (144), Expect = 1e-07
Identities = 27/56 (48%), Positives = 42/56 (75%)
Frame = -1
Query: 541 LSAFQIQENINLFCQTRDNIFKIMNDLCDTPEVMKQMPPLPVKVNDELANSILPRT 374
LSA ++Q+NI+LF Q R+NI I+ D+ + P +M +MP LPV +ND+LA+++L T
Sbjct: 183 LSACKLQDNISLFHQARNNISAILTDMKEMPGIMSRMPALPVSINDDLASNLLSST 238
[29][TOP]
>UniRef100_B4FNN3 Putative uncharacterized protein n=1 Tax=Zea mays
RepID=B4FNN3_MAIZE
Length = 295
Score = 57.8 bits (138), Expect = 5e-07
Identities = 26/53 (49%), Positives = 34/53 (64%)
Frame = -1
Query: 529 QIQENINLFCQTRDNIFKIMNDLCDTPEVMKQMPPLPVKVNDELANSILPRTH 371
Q NINLF R NI ++ +C P +M +MPPLPV V++ LA+ ILPR H
Sbjct: 226 QAHNNINLFHHIRRNINGLLQSMCQIPGIMSKMPPLPVSVDERLASYILPRAH 278
[30][TOP]
>UniRef100_A5CAA1 Putative uncharacterized protein n=1 Tax=Vitis vinifera
RepID=A5CAA1_VITVI
Length = 255
Score = 55.8 bits (133), Expect = 2e-06
Identities = 32/77 (41%), Positives = 43/77 (55%), Gaps = 25/77 (32%)
Frame = -1
Query: 538 SAFQIQENINLFCQTRDNI------------FKIMN-------------DLCDTPEVMKQ 434
S+ QI++NI+LFCQ RDN+ F +N +L D PE MK+
Sbjct: 168 SSLQIEDNISLFCQARDNLQVFNNHTTLWKQFSTLNISAKLICCNLSEINLKDEPEAMKK 227
Query: 433 MPPLPVKVNDELANSIL 383
MPPLP K+N+E+ NSIL
Sbjct: 228 MPPLPDKLNEEMINSIL 244
[31][TOP]
>UniRef100_Q94AP2 Putative uncharacterized protein At1g60670 n=1 Tax=Arabidopsis
thaliana RepID=Q94AP2_ARATH
Length = 254
Score = 55.5 bits (132), Expect = 2e-06
Identities = 23/53 (43%), Positives = 40/53 (75%)
Frame = -1
Query: 541 LSAFQIQENINLFCQTRDNIFKIMNDLCDTPEVMKQMPPLPVKVNDELANSIL 383
LS + Q+N++LF R+NI I+ND+ + P ++ +MPPLPV +N++LA+S++
Sbjct: 179 LSVCKPQDNVSLFYMARNNISAILNDMKEMPGIISRMPPLPVSINNDLASSLV 231
[32][TOP]
>UniRef100_O22705 F8A5.19 protein n=1 Tax=Arabidopsis thaliana RepID=O22705_ARATH
Length = 249
Score = 55.5 bits (132), Expect = 2e-06
Identities = 23/53 (43%), Positives = 40/53 (75%)
Frame = -1
Query: 541 LSAFQIQENINLFCQTRDNIFKIMNDLCDTPEVMKQMPPLPVKVNDELANSIL 383
LS + Q+N++LF R+NI I+ND+ + P ++ +MPPLPV +N++LA+S++
Sbjct: 174 LSVCKPQDNVSLFYMARNNISAILNDMKEMPGIISRMPPLPVSINNDLASSLV 226
[33][TOP]
>UniRef100_B6SVU7 Putative uncharacterized protein n=1 Tax=Zea mays
RepID=B6SVU7_MAIZE
Length = 298
Score = 54.7 bits (130), Expect = 4e-06
Identities = 25/53 (47%), Positives = 33/53 (62%)
Frame = -1
Query: 529 QIQENINLFCQTRDNIFKIMNDLCDTPEVMKQMPPLPVKVNDELANSILPRTH 371
Q NINLF R NI ++ +C P +M +MPPLPV V++ LA+ ILP H
Sbjct: 226 QAHNNINLFHHIRRNINGLLQSMCQIPGIMSKMPPLPVSVDERLASYILPCAH 278
[34][TOP]
>UniRef100_Q9SAC1 T16B5.4 n=1 Tax=Arabidopsis thaliana RepID=Q9SAC1_ARATH
Length = 273
Score = 53.5 bits (127), Expect = 9e-06
Identities = 23/50 (46%), Positives = 37/50 (74%)
Frame = -1
Query: 523 QENINLFCQTRDNIFKIMNDLCDTPEVMKQMPPLPVKVNDELANSILPRT 374
++NI+LF Q R+NI I+ D+ + P +M +MP LPV +ND+LA+++L T
Sbjct: 204 KDNISLFHQARNNISAILTDMKEMPGIMSRMPALPVSINDDLASNLLSST 253