The cDNA fragment was inserted at the XhoI-SacI site of the attBpBC vector.
The attBpBC vector is the pBC SK+ vector (Cm resistance;
http://www.stratagene. com/vectors/selection/plasmid1.htm) with sequences
including attB1 and attB2 sites
(CTCGAGCAGCTGAAGCTTTCACAAGTTTGTACAAAAAAGCAGGCTCTTC-5' end of cDNA,
GAGCTCTCACCACTTTGTACAAGAAAGCTGGGCGGCCGCTTTTTTTTTTTTTTTTTT-3' end of cDNA).