##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AlwNI Aor51HI AscI
Asp700I Asp718I AspI AviII AvrII
BamHI BanI BbeI BbrPI BbvCI
BciVI BfrI BglI BlnI BsaAI
BsaHI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BsrBI BsrFI BssHII
BstBI Cfr10I ClaI CpoI DraIII
DrdI DsaI EagI EclXI Eco47III
Eco52I EcoRV FseI FspI Hin1I
HindIII KasI KpnI KspI MfeI
MluI MroI MunI NaeI NarI
NcoI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
StuI SwaI Tth111I XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AccII 2017 CGCG CG^CG
AflIII 280 ACATGT A^C[GA][CT]GT
ApaI 3661 GGGCCC GGGCC^C
AvaI 3884 CTCGGG C^[CT]CG[GA]G
BalI 911 TGGCCA TGG^CCA
BcgI 1140 CGAGTGAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 359 TGATCA T^GATCA
BcnI 123 CCGGG CC^[GC]GG
BlpI 498 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 498 GCTCAGC GC^T[ACGT]AGC
BsaBI 208 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 535 ACCGGA [AT]^CCGG[AT]
BseRI 446 CTCCTC GAGGAG(10/8)
BsoBI 3884 CTCGGG C^[CT]CG[GA]G
BspLU11I 280 ACATGT A^CATGT
BsrDI 2851 CATTGC GCAATG(2/0)
BssSI 3573 CACGAG CACGAG(-5/-1)
BstAPI 1302 GCAAGTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 4268 GGTGACC G^GT[ACGT]ACC
BstPI 4268 GGTGACC G^GT[ACGT]ACC
BstUI 2017 CGCG CG^CG
BstXI 1805 CCAGTTAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 498 GCTCAGC GC^T[ACGT]AGC
EaeI 911 TGGCCA [CT]^GGCC[GA]
EcoNI 1986 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 4268 GGTGACC G^GT[ACGT]ACC
FauI 2653 GCGGG CCCGC(4/6)
FbaI 359 TGATCA T^GATCA
HaeII 4064 AGCGCC [GA]GCGC^[CT]
HpaI 1808 GTTAAC GTT^AAC
MamI 208 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 911 TGGCCA TGG^CCA
MscI 911 TGGCCA TGG^CCA
MvnI 2017 CGCG CG^CG
NciI 123 CCGGG CC^[GC]GG
SpeI 682 ACTAGT A^CTAGT
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AhdI 3483 GACCTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3764 GACACATTGTC
AseI 3012 ATTAAT AT^TAAT
3298 ATTAAT
AsnI 3012 ATTAAT AT^TAAT
3298 ATTAAT
AspEI 3483 GACCTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3764 GACACATTGTC
BpmI 1987 CTGGAG CTGGAG(16/14)
2068 CTGGAG
Bst1107I 240 GTATAC GTA^TAC
3395 GTATAC
BstZ17I 240 GTATAC GTA^TAC
3395 GTATAC
CfoI 2016 GCGC GCG^C
4065 GCGC
DraII 2048 GGGACCT [GA]G^G[ACGT]CC[CT]
2630 AGGTCCT
Eam1105I 3483 GACCTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3764 GACACATTGTC
EcoO109I 2048 GGGACCT [GA]G^G[ACGT]CC[CT]
2630 AGGTCCT
EcoT22I 2283 ATGCAT ATGCA^T
3322 ATGCAT
HapII 123 CCGG C^CGG
536 CCGG
HhaI 2016 GCGC GCG^C
4065 GCGC
HinP1I 2016 GCGC G^CGC
4065 GCGC
HincII 1808 GTTAAC GT[CT]^[GA]AC
4020 GTTGAC
HindII 1808 GTTAAC GT[CT]^[GA]AC
4020 GTTGAC
HpaII 123 CCGG C^CGG
536 CCGG
MspI 123 CCGG C^CGG
536 CCGG
NdeI 3401 CATATG CA^TATG
3838 CATATG
NsiI 2283 ATGCAT ATGCA^T
3322 ATGCAT
PpuMI 2048 GGGACCT [GA]G^G[AT]CC[CT]
2630 AGGTCCT
PshBI 3012 ATTAAT AT^TAAT
3298 ATTAAT
PvuII 380 CAGCTG CAG^CTG
2930 CAGCTG
SacI 802 GAGCTC GAGCT^C
1102 GAGCTC
SmlI 3377 CTCAAG C^T[CT][GA]AG
3953 CTTGAG
SspI 3224 AATATT AAT^ATT
4342 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
Alw44I AlwI AocI ApaLI ApoI
AspHI AvaII BanII BbsI BbvI
BfaI BglII BmyI BpuAI BsaI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I Bsp1407I
BspHI BspMI BsrGI BsrI BssKI
BstF5I BstNI BstYI Bsu36I Cac8I
Cfr13I DdeI DpnII DraI EarI
Eco81I EcoRI EcoRII EcoT14I Fnu4HI
FokI HaeIII HgaI HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MvaI MwoI NdeII
NlaIII NlaIV NspI PflMI PleI
PstI RcaI RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XbaI XcmI XhoII