################################## # NO CUT ENZYMES ( 55 enzymes) # ################################## AatII AclI AflII AscI AviII BamHI BbeI BbrPI BfrI BglII BsaAI BsiWI Bsp1407I BspDI BsrDI BsrGI BssHII Bst1107I BstBI BstZ17I ClaI CpoI EcoRI FseI FspI KasI KspI MfeI MluI MunI NarI NotI NruI NspV PacI PaeR7I PmaCI PmeI PmlI Psp1406I PvuI RsrII SacII SalI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SspBI SwaI XhoI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccIII 717 TCCGGA T^CCGGA AcyI 3042 GGCGTC G[GA]^CG[CT]C AgeI 6498 ACCGGT A^CCGGT ApaI 4232 GGGCCC GGGCC^C AseI 196 ATTAAT AT^TAAT AsnI 196 ATTAAT AT^TAAT Asp700I 4429 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BcgI 739 GCAGTATCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsaBI 4657 GATATGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 3042 GGCGTC G[GA]^CG[CT]C BseAI 717 TCCGGA T^CCGGA BsmBI 1986 CGTCTC CGTCTC(1/5) BspEI 717 TCCGGA T^CCGGA BspLU11I 2977 ACATGT A^CATGT BsrBI 5167 GAGCGG CCGCTC(-3/-3) DrdI 1301 GACACTGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 5517 CGGCCG C^GGCCG EclXI 5517 CGGCCG C^GGCCG Eco52I 5517 CGGCCG C^GGCCG EcoRV 2439 GATATC GAT^ATC Hin1I 3042 GGCGTC G[GA]^CG[CT]C HpaI 3961 GTTAAC GTT^AAC MamI 4657 GATATGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 717 TCCGGA T^CCGGA NaeI 5515 GCCGGC GCC^GGC NdeI 4324 CATATG CA^TATG NgoMIV 5515 GCCGGC G^CCGGC PinAI 6498 ACCGGT A^CCGGT PshBI 196 ATTAAT AT^TAAT SbfI 1788 CCTGCAGG CCTGCA^GG SmaI 2879 CCCGGG CCC^GGG Sse8387I 1788 CCTGCAGG CCTGCA^GG SspI 6355 AATATT AAT^ATT XbaI 2435 TCTAGA T^CTAGA XmaCI 2879 CCCGGG C^CCGGG XmaI 2879 CCCGGG C^CCGGG XmnI 4429 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### Acc65I 5639 GGTACC G^GTACC 6289 GGTACC AhdI 286 GACTGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 626 GACCTGGAGTC Alw44I 798 GTGCAC G^TGCAC 916 GTGCAC ApaLI 798 GTGCAC G^TGCAC 916 GTGCAC Asp718I 5639 GGTACC G^GTACC 6289 GGTACC AspEI 286 GACTGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 626 GACCTGGAGTC AspI 209 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4283 GACTCAGTC BbvCI 5188 GCTGAGG CCTCAGC(-5/-2) 6135 GCTGAGG BciVI 3631 GTATCC GTATCC(6/5) 4975 GTATCC BclI 682 TGATCA T^GATCA 4129 TGATCA BsiEI 5517 CGGCCG CG[GA][CT]^CG 6711 CGACCG BsmI 4963 GAATGC GAATGC(1/-1) 4433 GCATTC BsrFI 5515 GCCGGC [GA]^CCGG[CT] 6498 ACCGGT BstYI 4447 AGATCC [GA]^GATC[CT] 5784 AGATCC Cfr10I 5515 GCCGGC [GA]^CCGG[CT] 6498 ACCGGT Eam1105I 286 GACTGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 626 GACCTGGAGTC EcoNI 6070 CCTCCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 6131 CCTTGCTGAGG FbaI 682 TGATCA T^GATCA 4129 TGATCA HgaI 1008 GACGC GACGC(5/10) 3043 GCGTC KpnI 5639 GGTACC GGTAC^C 6289 GGTACC MflI 4447 AGATCC [GA]^GATC[CT] 5784 AGATCC PflFI 209 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4283 GACTCAGTC SapI 519 GAAGAGC GCTCTTC(1/4) 763 GAAGAGC SphI 5362 GCATGC GCATG^C 5740 GCATGC Tth111I 209 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4283 GACTCAGTC XhoII 4447 AGATCC [GA]^GATC[CT] 5784 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 140 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI AocI Aor51HI ApoI AspHI AvaI AvaII AvrII BalI BanI BanII BbsI BbvI BcnI BfaI BglI BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspHI BspMI BsrI BssKI BssSI BstAPI BstEII BstF5I BstNI BstPI BstUI BstXI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DraIII DsaI EaeI EarI Eco47III Eco81I EcoO109I EcoO65I EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NheI NlaIII NlaIV NsiI NspI PflMI PleI PpuMI PshAI PstI PvuII RcaI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI