################################## # NO CUT ENZYMES ( 75 enzymes) # ################################## AccI AclI AgeI AhdI Aor51HI AscI Asp700I AspEI AvaI BcgI BsaBI BsiEI BsiWI BsoBI Bsp1407I BspDI BspHI BspLU11I BsrFI BsrGI BssHII Bst1107I BstBI BstZ17I Cfr10I ClaI CpoI DraI EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoRV FseI HpaI KspI MamI MfeI MluI MunI NaeI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmeI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AatII 4234 GACGTC GACGT^C Acc65I 336 GGTACC G^GTACC AccII 362 CGCG CG^CG AcsI 3009 GAATTT [GA]^AATT[CT] ApaI 4248 GGGCCC GGGCC^C ApoI 3009 GAATTT [GA]^AATT[CT] AseI 1756 ATTAAT AT^TAAT AsnI 1756 ATTAAT AT^TAAT Asp718I 336 GGTACC G^GTACC AspI 2262 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 244 TGCGCA TGC^GCA AvrII 3240 CCTAGG C^CTAGG BamHI 1874 GGATCC G^GATCC BbeI 3621 GGCGCC GGCGC^C BglII 1298 AGATCT A^GATCT BlnI 3240 CCTAGG C^CTAGG BsmBI 3779 CGTCTC CGTCTC(1/5) BssSI 987 CTCGTG CACGAG(-5/-1) BstEII 1652 GGTGACC G^GT[ACGT]ACC BstPI 1652 GGTGACC G^GT[ACGT]ACC BstUI 362 CGCG CG^CG DrdI 396 GACTCCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 1652 GGTGACC G^GT[ACGT]ACC EcoT22I 3497 ATGCAT ATGCA^T FauI 363 GCGGG CCCGC(4/6) FspI 244 TGCGCA TGC^GCA HaeII 3621 GGCGCC [GA]GCGC^[CT] KasI 3621 GGCGCC G^GCGCC KpnI 336 GGTACC GGTAC^C MvnI 362 CGCG CG^CG NarI 3621 GGCGCC GG^CGCC NsiI 3497 ATGCAT ATGCA^T PflFI 2262 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PshBI 1756 ATTAAT AT^TAAT ScaI 1483 AGTACT AGT^ACT SfoI 3621 GGCGCC GGC^GCC TaqI 664 TCGA T^CGA Tth111I 2262 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 664 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### AccIII 61 TCCGGA T^CCGGA 968 TCCGGA AocI 812 CCTTAGG CC^T[ACGT]AGG 3474 CCTCAGG BbrPI 93 CACGTG CAC^GTG 210 CACGTG BbvCI 744 GCTGAGG CCTCAGC(-5/-2) 1184 GCTGAGG BciVI 2317 GGATAC GTATCC(6/5) 2356 GGATAC BcnI 773 CCGGG CC^[GC]GG 2845 CCGGG BglI 1492 GCCACTAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 4124 GCCATGTGGGC BlpI 1684 GCTTAGC GC^T[ACGT]AGC 2453 GCTTAGC Bpu1102I 1684 GCTTAGC GC^T[ACGT]AGC 2453 GCTTAGC BsaAI 93 CACGTG [CT]AC^GT[GA] 210 CACGTG BsaI 44 GGTCTC GGTCTC(1/5) 693 GGTCTC BseAI 61 TCCGGA T^CCGGA 968 TCCGGA BsgI 1469 CTGCAC GTGCAG(16/14) 3460 CTGCAC BsmI 1055 GCATTC GAATGC(1/-1) 3835 GCATTC BspEI 61 TCCGGA T^CCGGA 968 TCCGGA BsrBI 332 GAGCGG CCGCTC(-3/-3) 3445 GAGCGG Bsu36I 812 CCTTAGG CC^T[ACGT]AGG 3474 CCTCAGG CelII 1684 GCTTAGC GC^T[ACGT]AGC 2453 GCTTAGC CfoI 245 GCGC GCG^C 3622 GCGC DraIII 2895 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG 3435 CACATTGTG Eco81I 812 CCTTAGG CC^T[ACGT]AGG 3474 CCTCAGG HhaI 245 GCGC GCG^C 3622 GCGC HinP1I 245 GCGC G^CGC 3622 GCGC HincII 11 GTTGAC GT[CT]^[GA]AC 528 GTCAAC HindII 11 GTTGAC GT[CT]^[GA]AC 528 GTCAAC HindIII 1682 AAGCTT A^AGCTT 4106 AAGCTT MroI 61 TCCGGA T^CCGGA 968 TCCGGA NciI 773 CCGGG CC^[GC]GG 2845 CCGGG NcoI 701 CCATGG C^CATGG 4243 CCATGG PflMI 3693 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4051 CCATCCTCTGG PmaCI 93 CACGTG CAC^GTG 210 CACGTG PmlI 93 CACGTG CAC^GTG 210 CACGTG SapI 1172 GAAGAGC GCTCTTC(1/4) 2728 GAAGAGC SphI 3495 GCATGC GCATG^C 3879 GCATGC Van91I 3693 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4051 CCATCCTCTGG XbaI 549 TCTAGA T^CTAGA 1505 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 108 enzymes) # ######################################### AciI AcyI AfaI AflII AflIII AluI Alw44I AlwI AlwNI ApaLI AspHI AvaII BalI BanI BanII BbsI BbvI BclI BfaI BfrI BmyI BpmI BpuAI BsaHI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Cac8I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FbaI Fnu4HI FokI HaeIII HapII HgaI Hin1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PleI PpuMI PshAI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XcmI XhoII