##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AccIII AcyI AgeI
AhdI Alw44I ApaI ApaLI AscI
Asp718I AspEI AvrII BamHI BbeI
BbrPI BlnI BsaHI BseAI BsiWI
BspEI BspLU11I BsrBI BsrDI BssHII
BstAPI BstBI CpoI DrdI Eam1105I
EcoNI EcoRV EcoT22I FseI Hin1I
KasI KpnI KspI MluI MroI
NaeI NarI NgoMIV NotI NruI
NsiI NspI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PvuII
RsrII SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SwaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccI 3677 GTATAC GT^[AC][GT]AC
AflII 535 CTTAAG C^TTAAG
AflIII 962 ACGTGT A^C[GA][CT]GT
AocI 2924 CCTAAGG CC^T[ACGT]AGG
Aor51HI 2760 AGCGCT AGC^GCT
AvaI 46 CTCGGG C^[CT]CG[GA]G
AviII 407 TGCGCA TGC^GCA
BbvCI 874 CCTCAGC CCTCAGC(-5/-2)
BciVI 2781 GGATAC GTATCC(6/5)
BcnI 1514 CCCGG CC^[GC]GG
BfrI 535 CTTAAG C^TTAAG
BglII 1372 AGATCT A^GATCT
BlpI 1553 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1553 GCTCAGC GC^T[ACGT]AGC
BsaAI 853 TACGTG [CT]AC^GT[GA]
BsaI 2287 GGTCTC GGTCTC(1/5)
BsaWI 2183 TCCGGT [AT]^CCGG[AT]
BseRI 1197 CTCCTC GAGGAG(10/8)
BsoBI 46 CTCGGG C^[CT]CG[GA]G
BspDI 460 ATCGAT AT^CGAT
BspHI 3929 TCATGA T^CATGA
BspMI 2690 GCAGGT ACCTGC(4/8)
Bst1107I 3677 GTATAC GTA^TAC
BstXI 633 CCACAGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 3677 GTATAC GTA^TAC
Bsu36I 2924 CCTAAGG CC^T[ACGT]AGG
CelII 1553 GCTCAGC GC^T[ACGT]AGC
ClaI 460 ATCGAT AT^CGAT
EagI 15 CGGCCG C^GGCCG
EclXI 15 CGGCCG C^GGCCG
Eco47III 2760 AGCGCT AGC^GCT
Eco52I 15 CGGCCG C^GGCCG
Eco81I 2924 CCTAAGG CC^T[ACGT]AGG
FspI 407 TGCGCA TGC^GCA
HaeII 2760 AGCGCT [GA]GCGC^[CT]
HindIII 532 AAGCTT A^AGCTT
HpaI 1152 GTTAAC GTT^AAC
MfeI 1379 CAATTG C^AATTG
MspA1I 1180 CAGCGG C[AC]G^C[GT]G
MunI 1379 CAATTG C^AATTG
NciI 1514 CCCGG CC^[GC]GG
NcoI 1692 CCATGG C^CATGG
NdeI 1578 CATATG CA^TATG
PshAI 889 GACACTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 2062 CTGCAG CTGCA^G
PvuI 458 CGATCG CGAT^CG
RcaI 3929 TCATGA T^CATGA
SapI 1715 GCTCTTC GCTCTTC(1/4)
StuI 3943 AGGCCT AGG^CCT
XcmI 1683 CCAAGTTTGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 41 enzymes) #
###################################
AccII 60 CGCG CG^CG
109 CGCG
AclI 434 AACGTT AA^CGTT
2213 AACGTT
AspI 1312 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
1526 GACAGGGTC
BcgI 74 CGAATTGAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1540 CGAATGGTGTGC
BglI 761 GCCATGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2921 GCCCCTAAGGC
BsaBI 2271 GATTTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3422 GATGATGATC
BsiEI 15 CGGCCG CG[GA][CT]^CG
458 CGATCG
BsmBI 1219 CGTCTC CGTCTC(1/5)
959 GAGACG
BsmI 925 GAATGC GAATGC(1/-1)
2544 GAATGC
Bsp1407I 1813 TGTACA T^GTACA
4191 TGTACA
BsrFI 17 GCCGGT [GA]^CCGG[CT]
1463 ACCGGC
BsrGI 1813 TGTACA T^GTACA
4191 TGTACA
BstEII 1191 GGTGACC G^GT[ACGT]ACC
3003 GGTCACC
BstPI 1191 GGTGACC G^GT[ACGT]ACC
3003 GGTCACC
BstUI 60 CGCG CG^CG
109 CGCG
BstYI 67 GGATCT [GA]^GATC[CT]
1372 AGATCT
Cfr10I 17 GCCGGT [GA]^CCGG[CT]
1463 ACCGGC
DraIII 1249 CACTCCGTG CAC[ACGT][ACGT][ACGT]^GTG
1788 CACATTGTG
EcoO65I 1191 GGTGACC G^GT[ACGT]ACC
3003 GGTCACC
EcoRI 2349 GAATTC G^AATTC
2643 GAATTC
FauI 7 GCGGG CCCGC(4/6)
61 GCGGG
HgaI 107 GACGC GACGC(5/10)
3791 GACGC
HincII 896 GTCAAC GT[CT]^[GA]AC
1152 GTTAAC
HindII 896 GTCAAC GT[CT]^[GA]AC
1152 GTTAAC
MamI 2271 GATTTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3422 GATGATGATC
MflI 67 GGATCT [GA]^GATC[CT]
1372 AGATCT
MvnI 60 CGCG CG^CG
109 CGCG
NheI 1500 GCTAGC G^CTAGC
2083 GCTAGC
PflFI 1312 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
1526 GACAGGGTC
PflMI 1017 CCAGGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3025 CCAACCCCTGG
PpuMI 1235 GGGACCC [GA]G^G[AT]CC[CT]
3618 AGGACCT
Psp1406I 434 AACGTT AA^CGTT
2213 AACGTT
SacI 2067 GAGCTC GAGCT^C
3925 GAGCTC
ScaI 3412 AGTACT AGT^ACT
3813 AGTACT
SfcI 2062 CTGCAG C^T[GA][CT]AG
3348 CTGTAG
SpeI 892 ACTAGT A^CTAGT
3685 ACTAGT
SspBI 1813 TGTACA T^GTACA
4191 TGTACA
Tth111I 1312 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
1526 GACAGGGTC
Van91I 1017 CCAGGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3025 CCAACCCCTGG
XbaI 162 TCTAGA T^CTAGA
2837 TCTAGA
XhoII 67 GGATCT [GA]^GATC[CT]
1372 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AseI AsnI Asp700I
AspHI AvaII BalI BanI BanII
BbsI BbvI BclI BfaI BmyI
BpmI BpuAI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BssSI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FbaI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MseI MslI
MspI MvaI MwoI NdeII NlaIII
NlaIV PleI PshBI RsaI Sau3AI
Sau96I ScrFI SfaNI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XmnI