################################## # NO CUT ENZYMES ( 77 enzymes) # ################################## AccII AccIII AcsI AflIII AgeI AhdI ApoI AscI AseI AsnI AspEI AspI AvrII BbrPI BcgI BclI BlnI BsaAI BseAI BsiWI BspDI BspEI BspLU11I BssHII BssSI BstBI BstUI ClaI CpoI DraIII Eam1105I EcoRI EcoRV EcoT22I FbaI FseI HpaI KspI MfeI MluI MroI MunI MvnI NdeI NotI NruI NsiI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshBI PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I Van91I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AatII 3147 GACGTC GACGT^C AflII 4293 CTTAAG C^TTAAG Aor51HI 1879 AGCGCT AGC^GCT Asp700I 2120 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 2437 TGCGCA TGC^GCA BamHI 1260 GGATCC G^GATCC BbeI 85 GGCGCC GGCGC^C BbsI 2124 GTCTTC GAAGAC(2/6) BciVI 2449 GTATCC GTATCC(6/5) BfrI 4293 CTTAAG C^TTAAG BpuAI 2124 GTCTTC GAAGAC(2/6) BsaBI 1214 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 2863 TCCGGT [AT]^CCGG[AT] BsiEI 27 CGGCCG CG[GA][CT]^CG BsmBI 71 GAGACG CGTCTC(1/5) BsmI 778 GAATGC GAATGC(1/-1) Bsp1407I 3837 TGTACA T^GTACA BsrFI 39 GCCGGC [GA]^CCGG[CT] BsrGI 3837 TGTACA T^GTACA Bst1107I 399 GTATAC GTA^TAC BstZ17I 399 GTATAC GTA^TAC Cfr10I 39 GCCGGC [GA]^CCGG[CT] DrdI 1504 GACTCTCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 27 CGGCCG C^GGCCG EclXI 27 CGGCCG C^GGCCG Eco47III 1879 AGCGCT AGC^GCT Eco52I 27 CGGCCG C^GGCCG FspI 2437 TGCGCA TGC^GCA KasI 85 GGCGCC G^GCGCC MamI 1214 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NaeI 39 GCCGGC GCC^GGC NarI 85 GGCGCC GG^CGCC NgoMIV 39 GCCGGC G^CCGGC NheI 728 GCTAGC G^CTAGC NspI 3287 ACATGC [GA]CATG^[CT] PaeR7I 2649 CTCGAG C^TCGAG PshAI 572 GACTCACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SfiI 1806 GGCCCCCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 85 GGCGCC GGC^GCC SspBI 3837 TGTACA T^GTACA XhoI 2649 CTCGAG C^TCGAG XmnI 2120 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AclI 297 AACGTT AA^CGTT 3156 AACGTT AcyI 85 GGCGCC G[GA]^CG[CT]C 3147 GACGTC BbvCI 375 GCTGAGG CCTCAGC(-5/-2) 2097 GCTGAGG BglII 245 AGATCT A^GATCT 1749 AGATCT BsaHI 85 GGCGCC G[GA]^CG[CT]C 3147 GACGTC BspHI 2617 TCATGA T^CATGA 4124 TCATGA BsrBI 2045 CCGCTC CCGCTC(-3/-3) 2937 GAGCGG BstEII 3053 GGTGACC G^GT[ACGT]ACC 4360 GGTTACC BstPI 3053 GGTGACC G^GT[ACGT]ACC 4360 GGTTACC BstXI 2243 CCAGGATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3473 CCAGGGGCATGG DraI 1247 TTTAAA TTT^AAA 2883 TTTAAA EcoNI 156 CCTCCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1565 CCTGAGTGAGG EcoO65I 3053 GGTGACC G^GT[ACGT]ACC 4360 GGTTACC HaeII 85 GGCGCC [GA]GCGC^[CT] 1879 AGCGCT HgaI 2380 GACGC GACGC(5/10) 2394 GACGC Hin1I 85 GGCGCC G[GA]^CG[CT]C 3147 GACGTC HincII 1326 GTCAAC GT[CT]^[GA]AC 1968 GTCAAC HindII 1326 GTCAAC GT[CT]^[GA]AC 1968 GTCAAC HindIII 336 AAGCTT A^AGCTT 4397 AAGCTT NcoI 3431 CCATGG C^CATGG 3704 CCATGG Psp1406I 297 AACGTT AA^CGTT 3156 AACGTT RcaI 2617 TCATGA T^CATGA 4124 TCATGA StuI 1959 AGGCCT AGG^CCT 3068 AGGCCT TaqI 565 TCGA T^CGA 2650 TCGA Tsp509I 1842 AATT ^AATT 4375 AATT TthHB8I 565 TCGA T^CGA 2650 TCGA XbaI 3161 TCTAGA T^CTAGA 3723 TCTAGA XcmI 3812 CCACTTAGGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4345 CCAGGGAACAGTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 111 enzymes) # ######################################### Acc65I AccI AciI AfaI AluI Alw44I AlwI AlwNI AocI ApaI ApaLI Asp718I AspHI AvaI AvaII BalI BanI BanII BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TfiI Tru9I TseI Tsp45I TspRI XhoII