##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AatII AclI AgeI Aor51HI AscI
AseI AsnI Asp700I AvrII BbeI
BcgI BglII BlnI BsaBI BsiWI
BspDI BspLU11I BssHII BssSI Bst1107I
BstBI BstZ17I ClaI CpoI DrdI
Eco47III EcoRV FseI HaeII KasI
KspI MamI MfeI MunI NarI
NotI NruI NspV PacI PinAI
PmeI PshBI Psp1406I PvuI RsrII
SacII SalI SapI SfoI SfuI
SgrAI SnaBI SpeI SphI SwaI
XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 4660 GGTACC G^GTACC
AccIII 2535 TCCGGA T^CCGGA
Alw44I 4784 GTGCAC G^TGCAC
AocI 4664 CCTCAGG CC^T[ACGT]AGG
ApaLI 4784 GTGCAC G^TGCAC
Asp718I 4660 GGTACC G^GTACC
AviII 2331 TGCGCA TGC^GCA
BamHI 4671 GGATCC G^GATCC
BbvCI 1286 CCTCAGC CCTCAGC(-5/-2)
BciVI 3938 GGATAC GTATCC(6/5)
BclI 2158 TGATCA T^GATCA
BlpI 1256 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1256 GCTGAGC GC^T[ACGT]AGC
BseAI 2535 TCCGGA T^CCGGA
BsiEI 2134 CGGCCG CG[GA][CT]^CG
BsmI 5081 GCATTC GAATGC(1/-1)
BspEI 2535 TCCGGA T^CCGGA
BspHI 1906 TCATGA T^CATGA
Bsu36I 4664 CCTCAGG CC^T[ACGT]AGG
CelII 1256 GCTGAGC GC^T[ACGT]AGC
EagI 2134 CGGCCG C^GGCCG
EclXI 2134 CGGCCG C^GGCCG
Eco52I 2134 CGGCCG C^GGCCG
Eco81I 4664 CCTCAGG CC^T[ACGT]AGG
EcoT22I 3798 ATGCAT ATGCA^T
FbaI 2158 TGATCA T^GATCA
FspI 2331 TGCGCA TGC^GCA
HincII 1919 GTTAAC GT[CT]^[GA]AC
HindII 1919 GTTAAC GT[CT]^[GA]AC
HindIII 4127 AAGCTT A^AGCTT
HpaI 1919 GTTAAC GTT^AAC
KpnI 4660 GGTACC GGTAC^C
MluI 2832 ACGCGT A^CGCGT
MroI 2535 TCCGGA T^CCGGA
NdeI 2034 CATATG CA^TATG
NheI 3014 GCTAGC G^CTAGC
NsiI 3798 ATGCAT ATGCA^T
PaeR7I 2608 CTCGAG C^TCGAG
PshAI 3845 GACTTAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1906 TCATGA T^CATGA
SbfI 3475 CCTGCAGG CCTGCA^GG
ScaI 4621 AGTACT AGT^ACT
SfiI 3403 GGCCCAGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 2217 CCCGGG CCC^GGG
Sse8387I 3475 CCTGCAGG CCTGCA^GG
XhoI 2608 CTCGAG C^TCGAG
XmaCI 2217 CCCGGG C^CCGGG
XmaI 2217 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 1490 GGCGTC G[GA]^CG[CT]C
2152 GGCGTC
AflII 3847 CTTAAG C^TTAAG
4124 CTTAAG
AspI 3986 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4503 GACCTCGTC
BbrPI 281 CACGTG CAC^GTG
3517 CACGTG
BfrI 3847 CTTAAG C^TTAAG
4124 CTTAAG
BsaHI 1490 GGCGTC G[GA]^CG[CT]C
2152 GGCGTC
BsaWI 2535 TCCGGA [AT]^CCGG[AT]
2997 TCCGGT
BsmBI 3417 CGTCTC CGTCTC(1/5)
1539 GAGACG
Bsp1407I 4078 TGTACA T^GTACA
4840 TGTACA
BsrBI 1447 CCGCTC CCGCTC(-3/-3)
3563 CCGCTC
BsrDI 796 CATTGC GCAATG(2/0)
3978 CATTGC
BsrGI 4078 TGTACA T^GTACA
4840 TGTACA
BstEII 163 GGTGACC G^GT[ACGT]ACC
3919 GGTGACC
BstPI 163 GGTGACC G^GT[ACGT]ACC
3919 GGTGACC
EcoO65I 163 GGTGACC G^GT[ACGT]ACC
3919 GGTGACC
Hin1I 1490 GGCGTC G[GA]^CG[CT]C
2152 GGCGTC
NaeI 3114 GCCGGC GCC^GGC
3784 GCCGGC
NgoMIV 3114 GCCGGC G^CCGGC
3784 GCCGGC
NspI 97 GCATGT [GA]CATG^[CT]
4520 GCATGT
PflFI 3986 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4503 GACCTCGTC
PflMI 2499 CCAAAGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3827 CCAGACCATGG
PmaCI 281 CACGTG CAC^GTG
3517 CACGTG
PmlI 281 CACGTG CAC^GTG
3517 CACGTG
SspBI 4078 TGTACA T^GTACA
4840 TGTACA
SspI 1382 AATATT AAT^ATT
2862 AATATT
Tth111I 3986 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4503 GACCTCGTC
Van91I 2499 CCAAAGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3827 CCAGACCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AhdI AluI AlwI AlwNI
ApaI ApoI AspEI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DraIII DsaI EaeI
Eam1105I EarI EcoNI EcoO109I EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XbaI
XcmI XhoII