################################## # NO CUT ENZYMES ( 72 enzymes) # ################################## Acc65I AclI AflII AscI AseI AsnI Asp718I AspI AvrII BamHI BbeI BciVI BclI BfrI BglII BlnI BsaBI BsiEI BsiWI BsmBI Bsp1407I BspDI BspHI BsrGI BssHII Bst1107I BstZ17I ClaI CpoI DraI DrdI EagI EclXI Eco52I EcoRV FbaI FseI HindIII HpaI KasI KpnI MamI MfeI MluI MunI NarI NotI NruI PacI PaeR7I PflFI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SfoI SgrAI SmaI SnaBI SpeI SphI SspBI SspI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AatII 70 GACGTC GACGT^C AccI 2165 GTCGAC GT^[AC][GT]AC AccII 11 CGCG CG^CG AflIII 3339 ACATGT A^C[GA][CT]GT AgeI 32 ACCGGT A^CCGGT AhdI 110 GACCTGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 3137 GTGCAC G^TGCAC AocI 3596 CCTGAGG CC^T[ACGT]AGG ApaLI 3137 GTGCAC G^TGCAC Asp700I 3049 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 110 GACCTGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 2864 CCCGAG C^[CT]CG[GA]G BbrPI 3359 CACGTG CAC^GTG BsmI 2549 GCATTC GAATGC(1/-1) BsoBI 2864 CCCGAG C^[CT]CG[GA]G BspLU11I 3339 ACATGT A^CATGT BsrBI 1181 GAGCGG CCGCTC(-3/-3) BssSI 3877 CACGAG CACGAG(-5/-1) BstBI 726 TTCGAA TT^CGAA BstEII 1820 GGTCACC G^GT[ACGT]ACC BstPI 1820 GGTCACC G^GT[ACGT]ACC BstUI 11 CGCG CG^CG Bsu36I 3596 CCTGAGG CC^T[ACGT]AGG Eam1105I 110 GACCTGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 3596 CCTGAGG CC^T[ACGT]AGG EcoO65I 1820 GGTCACC G^GT[ACGT]ACC EcoRI 1013 GAATTC G^AATTC EcoT22I 2144 ATGCAT ATGCA^T KspI 10 CCGCGG CCGC^GG MvnI 11 CGCG CG^CG NaeI 1552 GCCGGC GCC^GGC NgoMIV 1552 GCCGGC G^CCGGC NheI 3729 GCTAGC G^CTAGC NsiI 2144 ATGCAT ATGCA^T NspV 726 TTCGAA TT^CGAA PinAI 32 ACCGGT A^CCGGT PmaCI 3359 CACGTG CAC^GTG PmlI 3359 CACGTG CAC^GTG SacI 2348 GAGCTC GAGCT^C SacII 10 CCGCGG CCGC^GG SalI 2165 GTCGAC G^TCGAC SfuI 726 TTCGAA TT^CGAA XmnI 3049 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccIII 2946 TCCGGA T^CCGGA 3393 TCCGGA AcsI 1013 GAATTC [GA]^AATT[CT] 4235 AAATTT AcyI 70 GACGTC G[GA]^CG[CT]C 1415 GGCGTC Aor51HI 2850 AGCGCT AGC^GCT 3555 AGCGCT ApoI 1013 GAATTC [GA]^AATT[CT] 4235 AAATTT BbsI 355 GAAGAC GAAGAC(2/6) 2608 GAAGAC BcgI 2113 CGAGCTGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 606 GCACTGTTGTCG BpuAI 355 GAAGAC GAAGAC(2/6) 2608 GAAGAC BsaAI 911 CACGTA [CT]AC^GT[GA] 3359 CACGTG BsaHI 70 GACGTC G[GA]^CG[CT]C 1415 GGCGTC BseAI 2946 TCCGGA T^CCGGA 3393 TCCGGA BspEI 2946 TCCGGA T^CCGGA 3393 TCCGGA BstAPI 2245 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2480 GCAGGTACTGC BstYI 3207 AGATCC [GA]^GATC[CT] 3639 AGATCC Eco47III 2850 AGCGCT AGC^GCT 3555 AGCGCT Hin1I 70 GACGTC G[GA]^CG[CT]C 1415 GGCGTC HincII 2165 GTCGAC GT[CT]^[GA]AC 3972 GTTGAC HindII 2165 GTCGAC GT[CT]^[GA]AC 3972 GTTGAC MflI 3207 AGATCC [GA]^GATC[CT] 3639 AGATCC MroI 2946 TCCGGA T^CCGGA 3393 TCCGGA MseI 1356 TTAA T^TAA 4239 TTAA NcoI 807 CCATGG C^CATGG 4031 CCATGG NdeI 1716 CATATG CA^TATG 2667 CATATG ScaI 2340 AGTACT AGT^ACT 4227 AGTACT SexAI 675 ACCTGGT A^CC[AT]GGT 2382 ACCAGGT SfiI 865 GGCCTTTGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 3418 GGCCCGACTGGCC SmlI 1489 CTCAAG C^T[CT][GA]AG 2524 CTCAAG TfiI 2011 GAATC G^A[AT]TC 2943 GATTC Tru9I 1356 TTAA T^TAA 4239 TTAA XhoII 3207 AGATCC [GA]^GATC[CT] 3639 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 113 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI ApaI AspHI AvaII AviII BalI BanI BanII BbvCI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstXI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I SbfI ScrFI SfaNI SfcI Sse8387I StuI StyI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI