##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
AccIII AclI AgeI Alw44I ApaLI
AscI AseI AsnI AspI AvrII
BamHI BlnI BlpI Bpu1102I BseAI
BsiWI BspDI BspEI BspHI BssHII
BstBI CelII ClaI CpoI DrdI
FseI MfeI MluI MroI MunI
NcoI NheI NotI NruI NspV
PacI PflFI PinAI PmeI PshBI
Psp1406I RcaI RsrII SalI SbfI
SexAI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AatII 171 GACGTC GACGT^C
Acc65I 1736 GGTACC G^GTACC
AocI 2282 CCTCAGG CC^T[ACGT]AGG
ApaI 263 GGGCCC GGGCC^C
Asp700I 1920 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1736 GGTACC G^GTACC
AviII 2635 TGCGCA TGC^GCA
BbrPI 2427 CACGTG CAC^GTG
BbvCI 3297 CCTCAGC CCTCAGC(-5/-2)
BciVI 3949 GTATCC GTATCC(6/5)
BsaBI 2438 GATGGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1894 GAGACC GGTCTC(1/5)
BsaWI 1874 TCCGGT [AT]^CCGG[AT]
Bsp1407I 3765 TGTACA T^GTACA
BspMI 2016 ACCTGC ACCTGC(4/8)
BsrBI 1461 GAGCGG CCGCTC(-3/-3)
BsrFI 300 GCCGGC [GA]^CCGG[CT]
BsrGI 3765 TGTACA T^GTACA
BssSI 3992 CACGAG CACGAG(-5/-1)
Bst1107I 3575 GTATAC GTA^TAC
BstEII 2218 GGTCACC G^GT[ACGT]ACC
BstPI 2218 GGTCACC G^GT[ACGT]ACC
BstZ17I 3575 GTATAC GTA^TAC
Bsu36I 2282 CCTCAGG CC^T[ACGT]AGG
Cfr10I 300 GCCGGC [GA]^CCGG[CT]
DraIII 3751 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 2282 CCTCAGG CC^T[ACGT]AGG
EcoNI 2462 CCTGGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2218 GGTCACC G^GT[ACGT]ACC
EcoRI 3897 GAATTC G^AATTC
FspI 2635 TGCGCA TGC^GCA
KpnI 1736 GGTACC GGTAC^C
KspI 258 CCGCGG CCGC^GG
MamI 2438 GATGGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 300 GCCGGC GCC^GGC
NgoMIV 300 GCCGGC G^CCGGC
PaeR7I 2728 CTCGAG C^TCGAG
PflMI 2585 CCAAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2427 CACGTG CAC^GTG
PmlI 2427 CACGTG CAC^GTG
PshAI 3588 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 565 CGATCG CGAT^CG
SacII 258 CCGCGG CCGC^GG
SapI 3878 GAAGAGC GCTCTTC(1/4)
ScaI 3719 AGTACT AGT^ACT
SphI 2638 GCATGC GCATG^C
SspBI 3765 TGTACA T^GTACA
SspI 2888 AATATT AAT^ATT
Van91I 2585 CCAAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 2728 CTCGAG C^TCGAG
XmnI 1920 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AflII 2858 CTTAAG C^TTAAG
3081 CTTAAG
BbeI 57 GGCGCC GGCGC^C
226 GGCGCC
BbsI 1652 GAAGAC GAAGAC(2/6)
3354 GAAGAC
BcgI 1659 CGACAGTGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
69 GCAGCGCCGTCG
BclI 2051 TGATCA T^GATCA
3290 TGATCA
BfrI 2858 CTTAAG C^TTAAG
3081 CTTAAG
BglI 3014 GCCCAAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3834 GCCATGAGGGC
BglII 2006 AGATCT A^GATCT
2234 AGATCT
BpmI 342 CTGGAG CTGGAG(16/14)
477 CTGGAG
BpuAI 1652 GAAGAC GAAGAC(2/6)
3354 GAAGAC
BsaAI 2427 CACGTG [CT]AC^GT[GA]
3761 TACGTG
BsmI 1268 GAATGC GAATGC(1/-1)
1132 GCATTC
BspLU11I 787 ACATGT A^CATGT
1675 ACATGT
BsrDI 2702 CATTGC GCAATG(2/0)
1557 GCAATG
BstAPI 681 GCATTAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1400 GCATGAGATGC
BstXI 334 CCAACTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3970 CCACCAACTTGG
DraI 1455 TTTAAA TTT^AAA
3519 TTTAAA
EagI 255 CGGCCG C^GGCCG
2365 CGGCCG
EclXI 255 CGGCCG C^GGCCG
2365 CGGCCG
Eco52I 255 CGGCCG C^GGCCG
2365 CGGCCG
EcoT22I 1495 ATGCAT ATGCA^T
2941 ATGCAT
FbaI 2051 TGATCA T^GATCA
3290 TGATCA
HindIII 1138 AAGCTT A^AGCTT
4256 AAGCTT
HpaI 1679 GTTAAC GTT^AAC
3857 GTTAAC
KasI 57 GGCGCC G^GCGCC
226 GGCGCC
MslI 584 CACCAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1848 CATCCTCATG
NarI 57 GGCGCC GG^CGCC
226 GGCGCC
NdeI 3088 CATATG CA^TATG
3244 CATATG
NsiI 1495 ATGCAT ATGCA^T
2941 ATGCAT
SfoI 57 GGCGCC GGC^GCC
226 GGCGCC
SmaI 125 CCCGGG CCC^GGG
4120 CCCGGG
StuI 2241 AGGCCT AGG^CCT
3077 AGGCCT
XmaCI 125 CCCGGG C^CCGGG
4120 CCCGGG
XmaI 125 CCCGGG C^CCGGG
4120 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AhdI AluI AlwI
AlwNI Aor51HI ApoI AspEI AspHI
AvaI AvaII BalI BanI BanII
BbvI BcnI BfaI BmyI BsaHI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI Eam1105I EarI Eco47III EcoO109I
EcoRII EcoRV EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XbaI XcmI XhoII