################################## # NO CUT ENZYMES ( 68 enzymes) # ################################## AccIII AclI AgeI AhdI AocI ApaI AscI AseI AsnI AspEI BamHI BbrPI BbvCI BcgI BglII BlpI Bpu1102I BsaAI BseAI BsiWI BspDI BspEI BspHI BsrBI BssHII BssSI Bst1107I BstBI BstXI BstYI BstZ17I Bsu36I CelII ClaI DrdI Eam1105I Eco81I EcoRV FseI HindIII MfeI MflI MluI MroI MunI NotI NruI NspV PacI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI SalI SbfI SfuI SmaI SnaBI SpeI SphI Sse8387I XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### Acc65I 2767 GGTACC G^GTACC AflII 4520 CTTAAG C^TTAAG AflIII 3257 ACATGT A^C[GA][CT]GT Alw44I 1981 GTGCAC G^TGCAC ApaLI 1981 GTGCAC G^TGCAC Asp718I 2767 GGTACC G^GTACC AviII 410 TGCGCA TGC^GCA AvrII 3400 CCTAGG C^CTAGG BfrI 4520 CTTAAG C^TTAAG BlnI 3400 CCTAGG C^CTAGG BsaI 3556 GGTCTC GGTCTC(1/5) BsaWI 799 TCCGGT [AT]^CCGG[AT] BsgI 3663 CTGCAC GTGCAG(16/14) BsmBI 1365 GAGACG CGTCTC(1/5) BsmI 4428 GCATTC GAATGC(1/-1) Bsp1407I 2170 TGTACA T^GTACA BspLU11I 3257 ACATGT A^CATGT BspMI 1719 GCAGGT ACCTGC(4/8) BsrGI 2170 TGTACA T^GTACA BstEII 802 GGTCACC G^GT[ACGT]ACC BstPI 802 GGTCACC G^GT[ACGT]ACC EcoNI 1138 CCTGCTAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 802 GGTCACC G^GT[ACGT]ACC EcoRI 2705 GAATTC G^AATTC FspI 410 TGCGCA TGC^GCA KpnI 2767 GGTACC GGTAC^C KspI 104 CCGCGG CCGC^GG NcoI 836 CCATGG C^CATGG PaeR7I 1307 CTCGAG C^TCGAG PflMI 4079 CCAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 1028 CAGCTG CAG^CTG SacII 104 CCGCGG CCGC^GG SexAI 861 ACCTGGT A^CC[AT]GGT SfiI 132 GGCCCCTGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SgrAI 305 CGCCGGCG C[GA]^CCGG[CT]G SspBI 2170 TGTACA T^GTACA SspI 1753 AATATT AAT^ATT SwaI 3429 ATTTAAAT ATTT^AAAT Van91I 4079 CCAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 600 TCTAGA T^CTAGA XhoI 1307 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AatII 542 GACGTC GACGT^C 646 GACGTC AccI 2682 GTAGAC GT^[AC][GT]AC 2987 GTCTAC AlwI 3404 GGATC GGATC(4/5) 3469 GGATC AspI 860 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 4245 GACAGAGTC AvaI 60 CTCGGG C^[CT]CG[GA]G 1307 CTCGAG BalI 578 TGGCCA TGG^CCA 1673 TGGCCA BclI 3489 TGATCA T^GATCA 4276 TGATCA BsiEI 35 CGGCCG CG[GA][CT]^CG 140 CGGCCG BsoBI 60 CTCGGG C^[CT]CG[GA]G 1307 CTCGAG CpoI 339 CGGACCG CG^G[AT]CCG 1161 CGGTCCG DsaI 104 CCGCGG C^C[GA][CT]GG 836 CCATGG EagI 35 CGGCCG C^GGCCG 140 CGGCCG EclXI 35 CGGCCG C^GGCCG 140 CGGCCG Eco52I 35 CGGCCG C^GGCCG 140 CGGCCG EcoT22I 3064 ATGCAT ATGCA^T 3570 ATGCAT FbaI 3489 TGATCA T^GATCA 4276 TGATCA HpaI 3836 GTTAAC GTT^AAC 4237 GTTAAC MluNI 578 TGGCCA TGG^CCA 1673 TGGCCA MscI 578 TGGCCA TGG^CCA 1673 TGGCCA NdeI 2930 CATATG CA^TATG 3408 CATATG NheI 1387 GCTAGC G^CTAGC 2975 GCTAGC NsiI 3064 ATGCAT ATGCA^T 3570 ATGCAT PflFI 860 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 4245 GACAGAGTC PpuMI 858 GGGACCT [GA]G^G[AT]CC[CT] 1692 GGGTCCT PshAI 642 GACGGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2413 GACTCTTGTC RsrII 339 CGGACCG CG^G[AT]CCG 1161 CGGTCCG SapI 3744 GAAGAGC GCTCTTC(1/4) 2341 GCTCTTC ScaI 661 AGTACT AGT^ACT 1455 AGTACT SmlI 1307 CTCGAG C^T[CT][GA]AG 4520 CTTAAG Tth111I 860 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 4245 GACAGAGTC XcmI 693 CCAGTCAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3132 CCATTAGTTTACTGG ######################################### # 3 OR MORE CUT ENZYMES ( 118 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwNI Aor51HI ApoI Asp700I AspHI AvaII BanI BanII BbeI BbsI BbvI BciVI BcnI BfaI BglI BmyI BpmI BpuAI BsaBI BsaHI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrDI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DraIII EaeI EarI Eco47III EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIII NlaIV NspI PleI PstI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XmnI