##################################
# NO CUT ENZYMES (   68 enzymes) #
##################################

AccIII      AclI        AgeI        AhdI        AocI        
ApaI        AscI        AseI        AsnI        AspEI       
BamHI       BbrPI       BbvCI       BcgI        BglII       
BlpI        Bpu1102I    BsaAI       BseAI       BsiWI       
BspDI       BspEI       BspHI       BsrBI       BssHII      
BssSI       Bst1107I    BstBI       BstXI       BstYI       
BstZ17I     Bsu36I      CelII       ClaI        DrdI        
Eam1105I    Eco81I      EcoRV       FseI        HindIII     
MfeI        MflI        MluI        MroI        MunI        
NotI        NruI        NspV        PacI        PinAI       
PmaCI       PmeI        PmlI        PshBI       Psp1406I    
PvuI        RcaI        SalI        SbfI        SfuI        
SmaI        SnaBI       SpeI        SphI        Sse8387I    
XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

Acc65I       2767  GGTACC               G^GTACC
AflII        4520  CTTAAG               C^TTAAG
AflIII       3257  ACATGT               A^C[GA][CT]GT
Alw44I       1981  GTGCAC               G^TGCAC
ApaLI        1981  GTGCAC               G^TGCAC
Asp718I      2767  GGTACC               G^GTACC
AviII         410  TGCGCA               TGC^GCA
AvrII        3400  CCTAGG               C^CTAGG
BfrI         4520  CTTAAG               C^TTAAG
BlnI         3400  CCTAGG               C^CTAGG
BsaI         3556  GGTCTC               GGTCTC(1/5)
BsaWI         799  TCCGGT               [AT]^CCGG[AT]
BsgI         3663  CTGCAC               GTGCAG(16/14)
BsmBI        1365  GAGACG               CGTCTC(1/5)
BsmI         4428  GCATTC               GAATGC(1/-1)
Bsp1407I     2170  TGTACA               T^GTACA
BspLU11I     3257  ACATGT               A^CATGT
BspMI        1719  GCAGGT               ACCTGC(4/8)
BsrGI        2170  TGTACA               T^GTACA
BstEII        802  GGTCACC              G^GT[ACGT]ACC
BstPI         802  GGTCACC              G^GT[ACGT]ACC
EcoNI        1138  CCTGCTAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       802  GGTCACC              G^GT[ACGT]ACC
EcoRI        2705  GAATTC               G^AATTC
FspI          410  TGCGCA               TGC^GCA
KpnI         2767  GGTACC               GGTAC^C
KspI          104  CCGCGG               CCGC^GG
NcoI          836  CCATGG               C^CATGG
PaeR7I       1307  CTCGAG               C^TCGAG
PflMI        4079  CCAAGTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII        1028  CAGCTG               CAG^CTG
SacII         104  CCGCGG               CCGC^GG
SexAI         861  ACCTGGT              A^CC[AT]GGT
SfiI          132  GGCCCCTGCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI         305  CGCCGGCG             C[GA]^CCGG[CT]G
SspBI        2170  TGTACA               T^GTACA
SspI         1753  AATATT               AAT^ATT
SwaI         3429  ATTTAAAT             ATTT^AAAT
Van91I       4079  CCAAGTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI          600  TCTAGA               T^CTAGA
XhoI         1307  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AatII         542  GACGTC               GACGT^C
              646  GACGTC              
AccI         2682  GTAGAC               GT^[AC][GT]AC
             2987  GTCTAC              
AlwI         3404  GGATC                GGATC(4/5)
             3469  GGATC               
AspI          860  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4245  GACAGAGTC           
AvaI           60  CTCGGG               C^[CT]CG[GA]G
             1307  CTCGAG              
BalI          578  TGGCCA               TGG^CCA
             1673  TGGCCA              
BclI         3489  TGATCA               T^GATCA
             4276  TGATCA              
BsiEI          35  CGGCCG               CG[GA][CT]^CG
              140  CGGCCG              
BsoBI          60  CTCGGG               C^[CT]CG[GA]G
             1307  CTCGAG              
CpoI          339  CGGACCG              CG^G[AT]CCG
             1161  CGGTCCG             
DsaI          104  CCGCGG               C^C[GA][CT]GG
              836  CCATGG              
EagI           35  CGGCCG               C^GGCCG
              140  CGGCCG              
EclXI          35  CGGCCG               C^GGCCG
              140  CGGCCG              
Eco52I         35  CGGCCG               C^GGCCG
              140  CGGCCG              
EcoT22I      3064  ATGCAT               ATGCA^T
             3570  ATGCAT              
FbaI         3489  TGATCA               T^GATCA
             4276  TGATCA              
HpaI         3836  GTTAAC               GTT^AAC
             4237  GTTAAC              
MluNI         578  TGGCCA               TGG^CCA
             1673  TGGCCA              
MscI          578  TGGCCA               TGG^CCA
             1673  TGGCCA              
NdeI         2930  CATATG               CA^TATG
             3408  CATATG              
NheI         1387  GCTAGC               G^CTAGC
             2975  GCTAGC              
NsiI         3064  ATGCAT               ATGCA^T
             3570  ATGCAT              
PflFI         860  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4245  GACAGAGTC           
PpuMI         858  GGGACCT              [GA]G^G[AT]CC[CT]
             1692  GGGTCCT             
PshAI         642  GACGGACGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             2413  GACTCTTGTC          
RsrII         339  CGGACCG              CG^G[AT]CCG
             1161  CGGTCCG             
SapI         3744  GAAGAGC              GCTCTTC(1/4)
             2341  GCTCTTC             
ScaI          661  AGTACT               AGT^ACT
             1455  AGTACT              
SmlI         1307  CTCGAG               C^T[CT][GA]AG
             4520  CTTAAG              
Tth111I       860  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4245  GACAGAGTC           
XcmI          693  CCAGTCAGAAAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             3132  CCATTAGTTTACTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  118 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AluI        AlwNI       Aor51HI     ApoI        Asp700I     
AspHI       AvaII       BanI        BanII       BbeI        
BbsI        BbvI        BciVI       BcnI        BfaI        
BglI        BmyI        BpmI        BpuAI       BsaBI       
BsaHI       BsaJI       BseRI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BsrDI       
BsrFI       BsrI        BssKI       BstAPI      BstF5I      
BstNI       BstUI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraI        DraII       
DraIII      EaeI        EarI        Eco47III    EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HinfI       
HpaII       HphI        ItaI        KasI        Ksp632I     
MaeI        MaeII       MaeIII      MamI        MboI        
MboII       MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NaeI        
NarI        NciI        NdeII       NgoMIV      NlaIII      
NlaIV       NspI        PleI        PstI        RsaI        
SacI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SfoI        StuI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XmnI