##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AccIII AclI AgeI AhdI AocI
ApaI AscI AseI AsnI AspEI
BamHI BbrPI BbvCI BcgI BglII
BlpI Bpu1102I BsaAI BseAI BsiWI
BspDI BspEI BspHI BsrBI BssHII
BssSI Bst1107I BstBI BstXI BstYI
BstZ17I Bsu36I CelII ClaI DrdI
Eam1105I Eco81I EcoRV FseI HindIII
MfeI MflI MluI MroI MunI
NotI NruI NspV PacI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI SalI SbfI SfuI
SmaI SnaBI SpeI SphI Sse8387I
XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 2767 GGTACC G^GTACC
AflII 4520 CTTAAG C^TTAAG
AflIII 3257 ACATGT A^C[GA][CT]GT
Alw44I 1981 GTGCAC G^TGCAC
ApaLI 1981 GTGCAC G^TGCAC
Asp718I 2767 GGTACC G^GTACC
AviII 410 TGCGCA TGC^GCA
AvrII 3400 CCTAGG C^CTAGG
BfrI 4520 CTTAAG C^TTAAG
BlnI 3400 CCTAGG C^CTAGG
BsaI 3556 GGTCTC GGTCTC(1/5)
BsaWI 799 TCCGGT [AT]^CCGG[AT]
BsgI 3663 CTGCAC GTGCAG(16/14)
BsmBI 1365 GAGACG CGTCTC(1/5)
BsmI 4428 GCATTC GAATGC(1/-1)
Bsp1407I 2170 TGTACA T^GTACA
BspLU11I 3257 ACATGT A^CATGT
BspMI 1719 GCAGGT ACCTGC(4/8)
BsrGI 2170 TGTACA T^GTACA
BstEII 802 GGTCACC G^GT[ACGT]ACC
BstPI 802 GGTCACC G^GT[ACGT]ACC
EcoNI 1138 CCTGCTAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 802 GGTCACC G^GT[ACGT]ACC
EcoRI 2705 GAATTC G^AATTC
FspI 410 TGCGCA TGC^GCA
KpnI 2767 GGTACC GGTAC^C
KspI 104 CCGCGG CCGC^GG
NcoI 836 CCATGG C^CATGG
PaeR7I 1307 CTCGAG C^TCGAG
PflMI 4079 CCAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 1028 CAGCTG CAG^CTG
SacII 104 CCGCGG CCGC^GG
SexAI 861 ACCTGGT A^CC[AT]GGT
SfiI 132 GGCCCCTGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 305 CGCCGGCG C[GA]^CCGG[CT]G
SspBI 2170 TGTACA T^GTACA
SspI 1753 AATATT AAT^ATT
SwaI 3429 ATTTAAAT ATTT^AAAT
Van91I 4079 CCAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 600 TCTAGA T^CTAGA
XhoI 1307 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AatII 542 GACGTC GACGT^C
646 GACGTC
AccI 2682 GTAGAC GT^[AC][GT]AC
2987 GTCTAC
AlwI 3404 GGATC GGATC(4/5)
3469 GGATC
AspI 860 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4245 GACAGAGTC
AvaI 60 CTCGGG C^[CT]CG[GA]G
1307 CTCGAG
BalI 578 TGGCCA TGG^CCA
1673 TGGCCA
BclI 3489 TGATCA T^GATCA
4276 TGATCA
BsiEI 35 CGGCCG CG[GA][CT]^CG
140 CGGCCG
BsoBI 60 CTCGGG C^[CT]CG[GA]G
1307 CTCGAG
CpoI 339 CGGACCG CG^G[AT]CCG
1161 CGGTCCG
DsaI 104 CCGCGG C^C[GA][CT]GG
836 CCATGG
EagI 35 CGGCCG C^GGCCG
140 CGGCCG
EclXI 35 CGGCCG C^GGCCG
140 CGGCCG
Eco52I 35 CGGCCG C^GGCCG
140 CGGCCG
EcoT22I 3064 ATGCAT ATGCA^T
3570 ATGCAT
FbaI 3489 TGATCA T^GATCA
4276 TGATCA
HpaI 3836 GTTAAC GTT^AAC
4237 GTTAAC
MluNI 578 TGGCCA TGG^CCA
1673 TGGCCA
MscI 578 TGGCCA TGG^CCA
1673 TGGCCA
NdeI 2930 CATATG CA^TATG
3408 CATATG
NheI 1387 GCTAGC G^CTAGC
2975 GCTAGC
NsiI 3064 ATGCAT ATGCA^T
3570 ATGCAT
PflFI 860 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4245 GACAGAGTC
PpuMI 858 GGGACCT [GA]G^G[AT]CC[CT]
1692 GGGTCCT
PshAI 642 GACGGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2413 GACTCTTGTC
RsrII 339 CGGACCG CG^G[AT]CCG
1161 CGGTCCG
SapI 3744 GAAGAGC GCTCTTC(1/4)
2341 GCTCTTC
ScaI 661 AGTACT AGT^ACT
1455 AGTACT
SmlI 1307 CTCGAG C^T[CT][GA]AG
4520 CTTAAG
Tth111I 860 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4245 GACAGAGTC
XcmI 693 CCAGTCAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3132 CCATTAGTTTACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwNI Aor51HI ApoI Asp700I
AspHI AvaII BanI BanII BbeI
BbsI BbvI BciVI BcnI BfaI
BglI BmyI BpmI BpuAI BsaBI
BsaHI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrDI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DraIII EaeI EarI Eco47III EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NdeII NgoMIV NlaIII
NlaIV NspI PleI PstI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XmnI