################################## # NO CUT ENZYMES ( 86 enzymes) # ################################## Acc65I AclI AgeI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp718I AspI AvrII BalI BamHI BanI BbeI BbrPI BbvCI BciVI BglI BlnI BlpI Bpu1102I BsaBI BsiWI Bsp1407I BspDI BspLU11I BsrDI BsrGI Bst1107I BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraIII EagI EclXI Eco47III Eco52I EcoO65I EcoRV FseI HaeII KasI KpnI MamI MluI MluNI MscI NarI NdeI NotI NruI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RsrII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I SspBI SwaI Tth111I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AatII 2924 GACGTC GACGT^C AccI 1319 GTAGAC GT^[AC][GT]AC AccIII 859 TCCGGA T^CCGGA AflII 4135 CTTAAG C^TTAAG AflIII 606 ACGTGT A^C[GA][CT]GT AhdI 701 GACTCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 2049 GGGCCC GGGCC^C AspEI 701 GACTCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 2086 TGCGCA TGC^GCA BcgI 1550 CGAGTTAATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1510 TGATCA T^GATCA BfrI 4135 CTTAAG C^TTAAG BsaAI 2322 CACGTA [CT]AC^GT[GA] BsaWI 859 TCCGGA [AT]^CCGG[AT] BseAI 859 TCCGGA T^CCGGA BsiEI 278 CGACCG CG[GA][CT]^CG BspEI 859 TCCGGA T^CCGGA BsrBI 662 GAGCGG CCGCTC(-3/-3) BssHII 1604 GCGCGC G^CGCGC DraI 3614 TTTAAA TTT^AAA DrdI 425 GACCATGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 701 GACTCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 2774 GAATTC G^AATTC EcoT22I 3967 ATGCAT ATGCA^T FbaI 1510 TGATCA T^GATCA FspI 2086 TGCGCA TGC^GCA KspI 7 CCGCGG CCGC^GG MfeI 3703 CAATTG C^AATTG MroI 859 TCCGGA T^CCGGA MunI 3703 CAATTG C^AATTG NheI 2812 GCTAGC G^CTAGC NsiI 3967 ATGCAT ATGCA^T NspI 2259 GCATGC [GA]CATG^[CT] PaeR7I 1164 CTCGAG C^TCGAG PflMI 2858 CCAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SacII 7 CCGCGG CCGC^GG SapI 1375 GCTCTTC GCTCTTC(1/4) SpeI 3988 ACTAGT A^CTAGT SphI 2259 GCATGC GCATG^C SspI 3434 AATATT AAT^ATT StuI 2983 AGGCCT AGG^CCT TfiI 3666 GAATC G^A[AT]TC Van91I 2858 CCAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 1164 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AocI 482 CCTCAGG CC^T[ACGT]AGG 974 CCTCAGG AvaI 21 CCCGAG C^[CT]CG[GA]G 1164 CTCGAG BsmBI 3235 CGTCTC CGTCTC(1/5) 965 GAGACG BsmI 1056 GAATGC GAATGC(1/-1) 4464 GCATTC BsoBI 21 CCCGAG C^[CT]CG[GA]G 1164 CTCGAG BspHI 1474 TCATGA T^CATGA 2452 TCATGA Bsu36I 482 CCTCAGG CC^T[ACGT]AGG 974 CCTCAGG EaeI 282 CGGCCA [CT]^GGCC[GA] 778 TGGCCG Eco81I 482 CCTCAGG CC^T[ACGT]AGG 974 CCTCAGG HindIII 1941 AAGCTT A^AGCTT 2509 AAGCTT MspA1I 7 CCGCGG C[AC]G^C[GT]G 133 CCGCTG NaeI 182 GCCGGC GCC^GGC 252 GCCGGC NgoMIV 182 GCCGGC G^CCGGC 252 GCCGGC PpuMI 56 GGGTCCT [GA]G^G[AT]CC[CT] 2218 AGGTCCT RcaI 1474 TCATGA T^CATGA 2452 TCATGA XcmI 1599 CCATAGCGCGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2385 CCAAGCAAGCCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 112 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI Asp700I AspHI AvaII BanII BbsI BbvI BcnI BfaI BglII BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrFI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV PleI PstI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XbaI XhoII XmnI