################################## # NO CUT ENZYMES ( 79 enzymes) # ################################## Acc65I AccIII AclI AgeI Alw44I ApaLI AscI AseI AsnI Asp718I AspI AviII BbeI BbrPI BcgI BciVI BsaWI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssSI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI EagI EclXI Eco52I EcoNI EcoO65I FseI FspI HindIII HpaI KasI KpnI KspI MfeI MroI MunI NarI NdeI NotI NruI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI SspBI SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AflII 2202 CTTAAG C^TTAAG AflIII 931 ACGCGT A^C[GA][CT]GT BamHI 2103 GGATCC G^GATCC BbsI 344 GTCTTC GAAGAC(2/6) BbvCI 790 CCTCAGC CCTCAGC(-5/-2) BclI 1110 TGATCA T^GATCA BfrI 2202 CTTAAG C^TTAAG BglII 1484 AGATCT A^GATCT BlpI 1851 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1851 GCTCAGC GC^T[ACGT]AGC BpuAI 344 GTCTTC GAAGAC(2/6) BsaAI 512 TACGTG [CT]AC^GT[GA] BsaBI 1105 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 1872 CGACCG CG[GA][CT]^CG BsmI 1255 GAATGC GAATGC(1/-1) BsrBI 977 GAGCGG CCGCTC(-3/-3) BssHII 305 GCGCGC G^CGCGC BstXI 1391 CCAAAACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 1851 GCTCAGC GC^T[ACGT]AGC DrdI 173 GACTGGATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 1691 GAATTC G^AATTC EcoRV 659 GATATC GAT^ATC EcoT22I 684 ATGCAT ATGCA^T FauI 666 CCCGC CCCGC(4/6) FbaI 1110 TGATCA T^GATCA MamI 1105 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluI 931 ACGCGT A^CGCGT NheI 2831 GCTAGC G^CTAGC NsiI 684 ATGCAT ATGCA^T PflMI 2215 CCAGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 3110 AGGTCCC [GA]G^G[AT]CC[CT] SbfI 778 CCTGCAGG CCTGCA^GG SexAI 1089 ACCTGGT A^CC[AT]GGT SphI 249 GCATGC GCATG^C Sse8387I 778 CCTGCAGG CCTGCA^GG SspI 1046 AATATT AAT^ATT StuI 962 AGGCCT AGG^CCT Van91I 2215 CCAGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 3037 CCAGTGCCAGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AatII 1663 GACGTC GACGT^C 2128 GACGTC AhdI 2110 GACAAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2757 GACTTCCTGTC AocI 2167 CCTTAGG CC^T[ACGT]AGG 2317 CCTTAGG Aor51HI 2393 AGCGCT AGC^GCT 2660 AGCGCT Asp700I 1959 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3091 GAATTGATTC AspEI 2110 GACAAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2757 GACTTCCTGTC AspHI 611 GAGCTC G[AT]GC[AT]^C 2303 GAGCTC AvrII 1232 CCTAGG C^CTAGG 3023 CCTAGG BalI 1188 TGGCCA TGG^CCA 2540 TGGCCA BanI 1840 GGCACC G^G[CT][GA]CC 3075 GGCACC BglI 492 GCCCGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1731 GCCCAGCCGGC BlnI 1232 CCTAGG C^CTAGG 3023 CCTAGG BsaI 8 GAGACC GGTCTC(1/5) 1119 GAGACC BseRI 2285 CTCCTC GAGGAG(10/8) 290 GAGGAG BsgI 486 GTGCAG GTGCAG(16/14) 1208 GTGCAG BsiHKAI 611 GAGCTC G[AT]GC[AT]^C 2303 GAGCTC BsrDI 2016 CATTGC GCAATG(2/0) 365 GCAATG Bsu36I 2167 CCTTAGG CC^T[ACGT]AGG 2317 CCTTAGG DraIII 731 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG 1879 CACTTTGTG Eam1105I 2110 GACAAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2757 GACTTCCTGTC Eco47III 2393 AGCGCT AGC^GCT 2660 AGCGCT Eco81I 2167 CCTTAGG CC^T[ACGT]AGG 2317 CCTTAGG HaeII 2393 AGCGCT [GA]GCGC^[CT] 2660 AGCGCT MluNI 1188 TGGCCA TGG^CCA 2540 TGGCCA MscI 1188 TGGCCA TGG^CCA 2540 TGGCCA NaeI 1736 GCCGGC GCC^GGC 2045 GCCGGC NgoMIV 1736 GCCGGC G^CCGGC 2045 GCCGGC PaeR7I 1289 CTCGAG C^TCGAG 2465 CTCGAG SacI 611 GAGCTC GAGCT^C 2303 GAGCTC SalI 27 GTCGAC G^TCGAC 2432 GTCGAC SmaI 493 CCCGGG CCC^GGG 1550 CCCGGG XhoI 1289 CTCGAG C^TCGAG 2465 CTCGAG XmaCI 493 CCCGGG C^CCGGG 1550 CCCGGG XmaI 493 CCCGGG C^CCGGG 1550 CCCGGG XmnI 1959 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3091 GAATTGATTC ######################################### # 3 OR MORE CUT ENZYMES ( 105 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI ApaI ApoI AvaI AvaII BanII BbvI BcnI BfaI BmyI BpmI BsaHI BsaJI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII