################################## # NO CUT ENZYMES ( 93 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AvaI AvrII BamHI BanI BbeI BbrPI BcgI BclI BlnI BsaAI BsaBI BsaHI BseAI BsiEI BsiWI BsmI BsoBI BspDI BspEI BspMI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I ClaI CpoI EagI EclXI Eco47III Eco52I Eco81I EcoO65I EcoT22I FbaI FseI HgaI Hin1I HpaI KasI KpnI MamI MfeI MroI MunI NarI NcoI NdeI NotI NruI NsiI NspV PacI PaeR7I PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacI SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AflII 1254 CTTAAG C^TTAAG AgeI 1079 ACCGGT A^CCGGT AspHI 1850 GAGCAC G[AT]GC[AT]^C AspI 915 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 458 TGGCCA TGG^CCA BciVI 53 GGATAC GTATCC(6/5) BfrI 1254 CTTAAG C^TTAAG BsaI 1016 GGTCTC GGTCTC(1/5) BsaWI 1079 ACCGGT [AT]^CCGG[AT] BsgI 1959 CTGCAC GTGCAG(16/14) BsiHKAI 1850 GAGCAC G[AT]GC[AT]^C BsmBI 920 CGTCTC CGTCTC(1/5) BspLU11I 2778 ACATGT A^CATGT BsrBI 829 CCGCTC CCGCTC(-3/-3) BssHII 314 GCGCGC G^CGCGC BssSI 1962 CACGAG CACGAG(-5/-1) DraIII 2470 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 2 CCGCGG C^C[GA][CT]GG EcoT14I 1657 CCTTGG C^C[AT][AT]GG HindIII 2371 AAGCTT A^AGCTT KspI 2 CCGCGG CCGC^GG MluI 2041 ACGCGT A^CGCGT MluNI 458 TGGCCA TGG^CCA MscI 458 TGGCCA TGG^CCA NaeI 465 GCCGGC GCC^GGC NgoMIV 465 GCCGGC G^CCGGC PflFI 915 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 197 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 1079 ACCGGT A^CCGGT PshAI 1038 GACCGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 1307 CTGCAG CTGCA^G SacII 2 CCGCGG CCGC^GG SapI 1980 GCTCTTC GCTCTTC(1/4) ScaI 1663 AGTACT AGT^ACT SpeI 2100 ACTAGT A^CTAGT SspI 2790 AATATT AAT^ATT StyI 1657 CCTTGG C^C[AT][AT]GG Tth111I 915 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 197 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AflIII 2041 ACGCGT A^C[GA][CT]GT 2778 ACATGT AhdI 779 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1110 GACTTCGTGTC AspEI 779 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1110 GACTTCGTGTC AviII 1330 TGCGCA TGC^GCA 2315 TGCGCA BbsI 1404 GAAGAC GAAGAC(2/6) 2607 GAAGAC BcnI 35 CCGGG CC^[GC]GG 2397 CCGGG BglI 460 GCCACGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1708 GCCAGCGAGGC BglII 2127 AGATCT A^GATCT 2673 AGATCT BlpI 401 GCTGAGC GC^T[ACGT]AGC 1364 GCTGAGC Bpu1102I 401 GCTGAGC GC^T[ACGT]AGC 1364 GCTGAGC BpuAI 1404 GAAGAC GAAGAC(2/6) 2607 GAAGAC BseRI 1275 CTCCTC GAGGAG(10/8) 2187 GAGGAG Bsp1407I 1201 TGTACA T^GTACA 1834 TGTACA BspHI 1678 TCATGA T^CATGA 2221 TCATGA BsrDI 766 CATTGC GCAATG(2/0) 1717 GCAATG BsrFI 465 GCCGGC [GA]^CCGG[CT] 1079 ACCGGT BsrGI 1201 TGTACA T^GTACA 1834 TGTACA BstAPI 89 GCAGCTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 324 GCAGAGTGTGC CelII 401 GCTGAGC GC^T[ACGT]AGC 1364 GCTGAGC Cfr10I 465 GCCGGC [GA]^CCGG[CT] 1079 ACCGGT DrdI 764 GACATTGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1967 GACACCCCTGTC EaeI 458 TGGCCA [CT]^GGCC[GA] 1105 TGGCCG Eam1105I 779 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1110 GACTTCGTGTC EcoRV 516 GATATC GAT^ATC 1293 GATATC FspI 1330 TGCGCA TGC^GCA 2315 TGCGCA HincII 482 GTCAAC GT[CT]^[GA]AC 1897 GTCAAC HindII 482 GTCAAC GT[CT]^[GA]AC 1897 GTCAAC NciI 35 CCGGG CC^[GC]GG 2397 CCGGG NheI 1469 GCTAGC G^CTAGC 2595 GCTAGC PpuMI 734 GGGACCC [GA]G^G[AT]CC[CT] 800 AGGACCT RcaI 1678 TCATGA T^CATGA 2221 TCATGA SspBI 1201 TGTACA T^GTACA 1834 TGTACA StuI 1181 AGGCCT AGG^CCT 2341 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 93 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AluI AlwI AlwNI ApoI Asp700I AvaII BanII BbvCI BbvI BfaI BmyI BpmI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII EarI EcoNI EcoO109I EcoRI EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV NspI PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmnI