##################################
# NO CUT ENZYMES ( 50 enzymes) #
##################################
AatII Acc65I AccI AclI AgeI
AhdI Aor51HI AscI Asp718I AspEI
AviII BciVI BsiWI BspDI BspHI
BssHII Bst1107I BstEII BstPI BstZ17I
ClaI DrdI Eam1105I Eco47III EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HpaI KpnI MluI NotI NruI
NsiI PacI PinAI PmeI Psp1406I
PvuI RcaI SalI SbfI SexAI
SgrAI SnaBI SpeI SphI Sse8387I
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AflII 3041 CTTAAG C^TTAAG
Alw44I 2310 GTGCAC G^TGCAC
ApaLI 2310 GTGCAC G^TGCAC
AseI 5776 ATTAAT AT^TAAT
AsnI 5776 ATTAAT AT^TAAT
Asp700I 1785 GAAACTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 5897 CCTAGG C^CTAGG
BcgI 3266 CGAGCATCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 3041 CTTAAG C^TTAAG
BglII 1441 AGATCT A^GATCT
BlnI 5897 CCTAGG C^CTAGG
BsaBI 657 GATCACGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 669 GAGACG CGTCTC(1/5)
BsmI 5313 GCATTC GAATGC(1/-1)
BspLU11I 884 ACATGT A^CATGT
BsrDI 367 GCAATG GCAATG(2/0)
BssSI 1425 CACGAG CACGAG(-5/-1)
CpoI 3355 CGGTCCG CG^G[AT]CCG
DraI 3464 TTTAAA TTT^AAA
HincII 2139 GTTGAC GT[CT]^[GA]AC
HindII 2139 GTTGAC GT[CT]^[GA]AC
HindIII 5407 AAGCTT A^AGCTT
MamI 657 GATCACGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NheI 3344 GCTAGC G^CTAGC
NspI 884 ACATGT [GA]CATG^[CT]
PaeR7I 4740 CTCGAG C^TCGAG
PshAI 1008 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 5776 ATTAAT AT^TAAT
PvuII 5091 CAGCTG CAG^CTG
RsrII 3355 CGGTCCG CG^G[AT]CCG
SapI 3764 GAAGAGC GCTCTTC(1/4)
ScaI 4478 AGTACT AGT^ACT
SfiI 1907 GGCCTTGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI 249 AGGCCT AGG^CCT
SwaI 3463 ATTTAAAT ATTT^AAAT
XhoI 4740 CTCGAG C^TCGAG
XmnI 1785 GAAACTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccIII 3923 TCCGGA T^CCGGA
4752 TCCGGA
AspI 2516 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3860 GACACTGTC
BamHI 1041 GGATCC G^GATCC
5901 GGATCC
BbeI 2061 GGCGCC GGCGC^C
4084 GGCGCC
BbvCI 2402 CCTCAGC CCTCAGC(-5/-2)
1272 GCTGAGG
BclI 656 TGATCA T^GATCA
743 TGATCA
BseAI 3923 TCCGGA T^CCGGA
4752 TCCGGA
BsiEI 2461 CGGCCG CG[GA][CT]^CG
2553 CGGCCG
BspEI 3923 TCCGGA T^CCGGA
4752 TCCGGA
BstBI 2538 TTCGAA TT^CGAA
2715 TTCGAA
EagI 2461 CGGCCG C^GGCCG
2553 CGGCCG
EclXI 2461 CGGCCG C^GGCCG
2553 CGGCCG
Eco52I 2461 CGGCCG C^GGCCG
2553 CGGCCG
FbaI 656 TGATCA T^GATCA
743 TGATCA
KasI 2061 GGCGCC G^GCGCC
4084 GGCGCC
KspI 23 CCGCGG CCGC^GG
141 CCGCGG
MfeI 1172 CAATTG C^AATTG
6020 CAATTG
MroI 3923 TCCGGA T^CCGGA
4752 TCCGGA
MunI 1172 CAATTG C^AATTG
6020 CAATTG
NaeI 1032 GCCGGC GCC^GGC
1913 GCCGGC
NarI 2061 GGCGCC GG^CGCC
4084 GGCGCC
NdeI 3019 CATATG CA^TATG
5049 CATATG
NgoMIV 1032 GCCGGC G^CCGGC
1913 GCCGGC
NspV 2538 TTCGAA TT^CGAA
2715 TTCGAA
PflFI 2516 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3860 GACACTGTC
SacI 3144 GAGCTC GAGCT^C
3767 GAGCTC
SacII 23 CCGCGG CCGC^GG
141 CCGCGG
SfoI 2061 GGCGCC GGC^GCC
4084 GGCGCC
SfuI 2538 TTCGAA TT^CGAA
2715 TTCGAA
SmaI 38 CCCGGG CCC^GGG
3250 CCCGGG
SspI 1731 AATATT AAT^ATT
6049 AATATT
Tth111I 2516 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3860 GACACTGTC
XbaI 1793 TCTAGA T^CTAGA
2973 TCTAGA
XmaCI 38 CCCGGG C^CCGGG
3250 CCCGGG
XmaI 38 CCCGGG C^CCGGG
3250 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApaI ApoI AspHI AvaI AvaII
BalI BanI BanII BbrPI BbsI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BspMI
BsrBI BsrFI BsrGI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
PflMI PleI PmaCI PmlI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII