################################## # NO CUT ENZYMES ( 58 enzymes) # ################################## AatII AclI AflII AgeI AscI AseI AsnI AviII AvrII BbrPI BfrI BlnI BsaBI BsiEI BsiWI BssHII BssSI BstBI CpoI DraIII EagI EclXI Eco52I EcoRV FseI FspI KspI MamI MfeI MluI MunI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI Sse8387I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 61 enzymes) # ################################### Acc65I 1378 GGTACC G^GTACC AccI 2384 GTATAC GT^[AC][GT]AC AccII 3 CGCG CG^CG AccIII 1128 TCCGGA T^CCGGA AcyI 109 GGCGCC G[GA]^CG[CT]C AflIII 3372 ACATGT A^C[GA][CT]GT AhdI 3294 GACTCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 4280 AGCGCT AGC^GCT Asp718I 1378 GGTACC G^GTACC AspEI 3294 GACTCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 5267 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 4907 CTCGGG C^[CT]CG[GA]G BbeI 109 GGCGCC GGCGC^C BbvCI 5348 CCTCAGC CCTCAGC(-5/-2) BcgI 3109 CGAATCCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 4465 TGATCA T^GATCA BglI 4145 GCCTGGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 155 GCTCAGC GC^T[ACGT]AGC Bpu1102I 155 GCTCAGC GC^T[ACGT]AGC BsaAI 471 CACGTA [CT]AC^GT[GA] BsaHI 109 GGCGCC G[GA]^CG[CT]C BsaWI 1128 TCCGGA [AT]^CCGG[AT] BseAI 1128 TCCGGA T^CCGGA BsmBI 3078 GAGACG CGTCTC(1/5) BsoBI 4907 CTCGGG C^[CT]CG[GA]G Bsp1407I 422 TGTACA T^GTACA BspDI 4300 ATCGAT AT^CGAT BspEI 1128 TCCGGA T^CCGGA BspHI 800 TCATGA T^CATGA BspLU11I 3372 ACATGT A^CATGT BsrBI 1441 GAGCGG CCGCTC(-3/-3) BsrGI 422 TGTACA T^GTACA Bst1107I 2384 GTATAC GTA^TAC BstAPI 3681 GCAGTAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 3 CGCG CG^CG BstZ17I 2384 GTATAC GTA^TAC CelII 155 GCTCAGC GC^T[ACGT]AGC ClaI 4300 ATCGAT AT^CGAT DrdI 2255 GACTTGAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 3294 GACTCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 4280 AGCGCT AGC^GCT FbaI 4465 TGATCA T^GATCA Hin1I 109 GGCGCC G[GA]^CG[CT]C KasI 109 GGCGCC G^GCGCC KpnI 1378 GGTACC GGTAC^C MroI 1128 TCCGGA T^CCGGA MvnI 3 CGCG CG^CG NarI 109 GGCGCC GG^CGCC NheI 3404 GCTAGC G^CTAGC PflFI 5267 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 4905 GACTCGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 800 TCATGA T^CATGA SapI 3926 GCTCTTC GCTCTTC(1/4) ScaI 1495 AGTACT AGT^ACT SfoI 109 GGCGCC GGC^GCC SspBI 422 TGTACA T^GTACA SspI 1829 AATATT AAT^ATT StuI 4735 AGGCCT AGG^CCT SwaI 1824 ATTTAAAT ATTT^AAAT Tth111I 5267 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 2466 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### Alw44I 954 GTGCAC G^TGCAC 2172 GTGCAC ApaI 76 GGGCCC GGGCC^C 829 GGGCCC ApaLI 954 GTGCAC G^TGCAC 2172 GTGCAC Asp700I 1326 GAACAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2704 GAAACTGTTC BamHI 1800 GGATCC G^GATCC 4797 GGATCC BglII 2881 AGATCT A^GATCT 3127 AGATCT BsrDI 945 CATTGC GCAATG(2/0) 4352 CATTGC BsrFI 136 GCCGGC [GA]^CCGG[CT] 4420 GCCGGC BstEII 1481 GGTTACC G^GT[ACGT]ACC 4504 GGTTACC BstPI 1481 GGTTACC G^GT[ACGT]ACC 4504 GGTTACC BstXI 3214 CCACTGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5728 CCATGTCTGTGG CfoI 110 GCGC GCG^C 4281 GCGC Cfr10I 136 GCCGGC [GA]^CCGG[CT] 4420 GCCGGC EcoO65I 1481 GGTTACC G^GT[ACGT]ACC 4504 GGTTACC EcoT22I 1718 ATGCAT ATGCA^T 4349 ATGCAT HaeII 109 GGCGCC [GA]GCGC^[CT] 4280 AGCGCT HgaI 870 GACGC GACGC(5/10) 3080 GACGC HhaI 110 GCGC GCG^C 4281 GCGC HinP1I 110 GCGC G^CGC 4281 GCGC HindIII 2075 AAGCTT A^AGCTT 2391 AAGCTT HpaI 409 GTTAAC GTT^AAC 5617 GTTAAC NaeI 136 GCCGGC GCC^GGC 4420 GCCGGC NdeI 4612 CATATG CA^TATG 5330 CATATG NgoMIV 136 GCCGGC G^CCGGC 4420 GCCGGC NsiI 1718 ATGCAT ATGCA^T 4349 ATGCAT PflMI 2557 CCACTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4306 CCAGAAGCTGG SacI 4227 GAGCTC GAGCT^C 4965 GAGCTC Van91I 2557 CCACTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4306 CCAGAAGCTGG XcmI 4802 CCAGAGTTAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4830 CCACAGCTCTTGTGG XmnI 1326 GAACAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2704 GAAACTGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 109 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI AocI ApoI AspHI AvaII BalI BanI BanII BbsI BbvI BciVI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Bsu36I Cac8I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII