##################################
# NO CUT ENZYMES ( 51 enzymes) #
##################################
AflIII AgeI AhdI Alw44I ApaI
ApaLI AscI AspEI AviII BbeI
BbrPI BglI BsiWI BspDI BspLU11I
BssHII Bst1107I BstZ17I ClaI CpoI
Eam1105I FseI FspI KasI MfeI
MluI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI PacI
PinAI PmaCI PmeI PmlI PvuI
RsrII SalI SbfI SfiI SfoI
SgrAI SnaBI SpeI Sse8387I SspI
SwaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccIII 4540 TCCGGA T^CCGGA
AclI 4240 AACGTT AA^CGTT
AflII 3815 CTTAAG C^TTAAG
Aor51HI 2542 AGCGCT AGC^GCT
BfrI 3815 CTTAAG C^TTAAG
BglII 3283 AGATCT A^GATCT
BsaAI 842 CACGTA [CT]AC^GT[GA]
BsaBI 1353 GATCTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 4540 TCCGGA T^CCGGA
Bsp1407I 3100 TGTACA T^GTACA
BspEI 4540 TCCGGA T^CCGGA
BsrFI 390 ACCGGC [GA]^CCGG[CT]
BsrGI 3100 TGTACA T^GTACA
BstAPI 1748 GCAGGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 4842 TTCGAA TT^CGAA
BstEII 3636 GGTAACC G^GT[ACGT]ACC
BstPI 3636 GGTAACC G^GT[ACGT]ACC
Cfr10I 390 ACCGGC [GA]^CCGG[CT]
DrdI 4148 GACGGAAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2542 AGCGCT AGC^GCT
EcoO65I 3636 GGTAACC G^GT[ACGT]ACC
HindIII 5904 AAGCTT A^AGCTT
HpaI 5072 GTTAAC GTT^AAC
KspI 82 CCGCGG CCGC^GG
MamI 1353 GATCTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 4540 TCCGGA T^CCGGA
NspV 4842 TTCGAA TT^CGAA
PshAI 5195 GACGGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 4240 AACGTT AA^CGTT
SacII 82 CCGCGG CCGC^GG
ScaI 3922 AGTACT AGT^ACT
SfuI 4842 TTCGAA TT^CGAA
SmaI 3259 CCCGGG CCC^GGG
SspBI 3100 TGTACA T^GTACA
XbaI 4295 TCTAGA T^CTAGA
XmaCI 3259 CCCGGG C^CCGGG
XmaI 3259 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AatII 3885 GACGTC GACGT^C
5199 GACGTC
Acc65I 2152 GGTACC G^GTACC
2347 GGTACC
AocI 1945 CCTTAGG CC^T[ACGT]AGG
6017 CCTTAGG
Asp718I 2152 GGTACC G^GTACC
2347 GGTACC
AspI 3930 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5139 GACCTGGTC
AvrII 245 CCTAGG C^CTAGG
4716 CCTAGG
BamHI 652 GGATCC G^GATCC
5111 GGATCC
BlnI 245 CCTAGG C^CTAGG
4716 CCTAGG
BlpI 1508 GCTGAGC GC^T[ACGT]AGC
2196 GCTGAGC
Bpu1102I 1508 GCTGAGC GC^T[ACGT]AGC
2196 GCTGAGC
BspHI 2740 TCATGA T^CATGA
3443 TCATGA
BsrBI 171 GAGCGG CCGCTC(-3/-3)
1683 GAGCGG
BssSI 3588 CACGAG CACGAG(-5/-1)
2626 CTCGTG
BstXI 3070 CCAAATTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4888 CCAAGTTCATGG
Bsu36I 1945 CCTTAGG CC^T[ACGT]AGG
6017 CCTTAGG
CelII 1508 GCTGAGC GC^T[ACGT]AGC
2196 GCTGAGC
DraI 4827 TTTAAA TTT^AAA
5821 TTTAAA
DraIII 1493 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
2136 CACTGGGTG
EagI 67 CGGCCG C^GGCCG
114 CGGCCG
EclXI 67 CGGCCG C^GGCCG
114 CGGCCG
Eco52I 67 CGGCCG C^GGCCG
114 CGGCCG
Eco81I 1945 CCTTAGG CC^T[ACGT]AGG
6017 CCTTAGG
KpnI 2152 GGTACC GGTAC^C
2347 GGTACC
NcoI 439 CCATGG C^CATGG
5656 CCATGG
PaeR7I 1776 CTCGAG C^TCGAG
2236 CTCGAG
PflFI 3930 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5139 GACCTGGTC
RcaI 2740 TCATGA T^CATGA
3443 TCATGA
SapI 5629 GAAGAGC GCTCTTC(1/4)
3146 GCTCTTC
SexAI 1660 ACCTGGT A^CC[AT]GGT
5140 ACCTGGT
SphI 240 GCATGC GCATG^C
5438 GCATGC
StuI 830 AGGCCT AGG^CCT
2983 AGGCCT
Tth111I 3930 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5139 GACCTGGTC
XhoI 1776 CTCGAG C^TCGAG
2236 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI ApoI
AseI AsnI Asp700I AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcgI BciVI BclI
BcnI BfaI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrDI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRI EcoRII EcoRV EcoT14I EcoT22I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NsiI NspI
PflMI PleI PpuMI PshBI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmnI