################################## # NO CUT ENZYMES ( 78 enzymes) # ################################## AatII AclI AflII AflIII AgeI AhdI AscI AseI AsnI Asp700I AspEI AspI AviII BalI BciVI BclI BfrI BglII BsaBI BsaI BsiWI Bsp1407I BspDI BspHI BspLU11I BsrGI BssSI Bst1107I BstBI BstXI BstZ17I ClaI Eam1105I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI MamI MfeI MluI MluNI MscI MunI NdeI NheI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI SacI SalI SapI ScaI SexAI SfiI SfuI SnaBI SspBI StuI SwaI Tth111I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### Acc65I 183 GGTACC G^GTACC AccI 2062 GTCTAC GT^[AC][GT]AC AccIII 1368 TCCGGA T^CCGGA Alw44I 2381 GTGCAC G^TGCAC Aor51HI 263 AGCGCT AGC^GCT ApaLI 2381 GTGCAC G^TGCAC Asp718I 183 GGTACC G^GTACC AvrII 1755 CCTAGG C^CTAGG BamHI 2538 GGATCC G^GATCC BbrPI 1192 CACGTG CAC^GTG BcgI 1050 CGAACGGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlnI 1755 CCTAGG C^CTAGG BsaAI 1192 CACGTG [CT]AC^GT[GA] BseAI 1368 TCCGGA T^CCGGA BsgI 1582 GTGCAG GTGCAG(16/14) BsmI 630 GCATTC GAATGC(1/-1) BspEI 1368 TCCGGA T^CCGGA BsrDI 480 CATTGC GCAATG(2/0) BssHII 1143 GCGCGC G^CGCGC BstAPI 1767 GCAGGGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DraIII 1570 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 1424 CGGCCG C^GGCCG EarI 2695 CTCTTC CTCTTC(1/4) EclXI 1424 CGGCCG C^GGCCG Eco47III 263 AGCGCT AGC^GCT Eco52I 1424 CGGCCG C^GGCCG HincII 871 GTCAAC GT[CT]^[GA]AC HindII 871 GTCAAC GT[CT]^[GA]AC KpnI 183 GGTACC GGTAC^C Ksp632I 2695 CTCTTC CTCTTC(1/4) KspI 1362 CCGCGG CCGC^GG MroI 1368 TCCGGA T^CCGGA NaeI 1244 GCCGGC GCC^GGC NgoMIV 1244 GCCGGC G^CCGGC NotI 1423 GCGGCCGC GC^GGCCGC PflMI 1569 CCACACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 1192 CACGTG CAC^GTG PmlI 1192 CACGTG CAC^GTG SacII 1362 CCGCGG CCGC^GG SgrAI 1131 CACCGGCG C[GA]^CCGG[CT]G SpeI 2727 ACTAGT A^CTAGT SphI 2599 GCATGC GCATG^C Van91I 1569 CCACACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AspHI 1122 GTGCTC G[AT]GC[AT]^C 2381 GTGCAC BpmI 1702 CTGGAG CTGGAG(16/14) 1939 CTGGAG BsiEI 1424 CGGCCG CG[GA][CT]^CG 1663 CGACCG BsiHKAI 1122 GTGCTC G[AT]GC[AT]^C 2381 GTGCAC BspMI 251 ACCTGC ACCTGC(4/8) 2776 ACCTGC BsrBI 1785 CCGCTC CCGCTC(-3/-3) 2214 GAGCGG BsrFI 1132 ACCGGC [GA]^CCGG[CT] 1244 GCCGGC Cfr10I 1132 ACCGGC [GA]^CCGG[CT] 1244 GCCGGC CpoI 596 CGGACCG CG^G[AT]CCG 1365 CGGTCCG DraI 2908 TTTAAA TTT^AAA 2928 TTTAAA DrdI 274 GACCTCAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2492 GACCTCTAAGTC EcoNI 294 CCTGCTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3082 CCTTCCTCAGG NcoI 1106 CCATGG C^CATGG 2238 CCATGG RsrII 596 CGGACCG CG^G[AT]CCG 1365 CGGTCCG SmlI 298 CTCAAG C^T[CT][GA]AG 472 CTCAAG SspI 25 AATATT AAT^ATT 2861 AATATT TaqI 362 TCGA T^CGA 716 TCGA TthHB8I 362 TCGA T^CGA 716 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 119 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI AocI ApaI ApoI AvaI AvaII BanI BanII BbeI BbsI BbvCI BbvI BcnI BfaI BglI BlpI BmyI Bpu1102I BpuAI BsaHI BsaJI BsaWI BseRI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BsrI BssKI BstEII BstF5I BstNI BstPI BstUI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI Eco81I EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I SbfI ScrFI SfaNI SfcI SfoI SmaI Sse8387I StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII XmaCI XmaI