##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AatII Acc65I AcyI AhdI Alw44I
Aor51HI ApaLI AscI Asp718I AspEI
AviII BbeI BcgI BlpI Bpu1102I
BsaBI BsaHI BsiEI BsiWI BspDI
BssHII CelII ClaI DrdI EagI
Eam1105I EclXI Eco47III Eco52I FseI
FspI Hin1I KasI KpnI MamI
NaeI NarI NgoMIV NheI NotI
NruI PacI PvuI SalI SbfI
SexAI SfiI SfoI SgrAI SpeI
SphI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccI 1028 GTATAC GT^[AC][GT]AC
AccIII 2357 TCCGGA T^CCGGA
AgeI 1903 ACCGGT A^CCGGT
ApaI 1698 GGGCCC GGGCC^C
BalI 2444 TGGCCA TGG^CCA
BanI 3324 GGCACC G^G[CT][GA]CC
BciVI 5919 GGATAC GTATCC(6/5)
BclI 1957 TGATCA T^GATCA
BglI 3054 GCCAACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 5986 AGATCT A^GATCT
BseAI 2357 TCCGGA T^CCGGA
BsmBI 6713 CGTCTC CGTCTC(1/5)
BspEI 2357 TCCGGA T^CCGGA
BsrDI 7067 CATTGC GCAATG(2/0)
BssSI 486 CACGAG CACGAG(-5/-1)
Bst1107I 1028 GTATAC GTA^TAC
BstBI 5363 TTCGAA TT^CGAA
BstEII 756 GGTGACC G^GT[ACGT]ACC
BstPI 756 GGTGACC G^GT[ACGT]ACC
BstZ17I 1028 GTATAC GTA^TAC
CpoI 1900 CGGACCG CG^G[AT]CCG
EaeI 2444 TGGCCA [CT]^GGCC[GA]
EcoO65I 756 GGTGACC G^GT[ACGT]ACC
EcoRV 2904 GATATC GAT^ATC
EcoT22I 2752 ATGCAT ATGCA^T
FbaI 1957 TGATCA T^GATCA
KspI 145 CCGCGG CCGC^GG
MfeI 286 CAATTG C^AATTG
MluI 1820 ACGCGT A^CGCGT
MluNI 2444 TGGCCA TGG^CCA
MroI 2357 TCCGGA T^CCGGA
MscI 2444 TGGCCA TGG^CCA
MunI 286 CAATTG C^AATTG
NsiI 2752 ATGCAT ATGCA^T
NspV 5363 TTCGAA TT^CGAA
PinAI 1903 ACCGGT A^CCGGT
PmeI 5798 GTTTAAAC GTTT^AAAC
RsrII 1900 CGGACCG CG^G[AT]CCG
SacII 145 CCGCGG CCGC^GG
SfuI 5363 TTCGAA TT^CGAA
SnaBI 4260 TACGTA TAC^GTA
SspI 5781 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AclI 4902 AACGTT AA^CGTT
7521 AACGTT
AocI 5570 CCTGAGG CC^T[ACGT]AGG
6660 CCTTAGG
AseI 5219 ATTAAT AT^TAAT
7723 ATTAAT
AsnI 5219 ATTAAT AT^TAAT
7723 ATTAAT
BbrPI 6147 CACGTG CAC^GTG
7447 CACGTG
BsaI 996 GGTCTC GGTCTC(1/5)
3916 GGTCTC
Bsp1407I 2259 TGTACA T^GTACA
3421 TGTACA
BsrGI 2259 TGTACA T^GTACA
3421 TGTACA
Bsu36I 5570 CCTGAGG CC^T[ACGT]AGG
6660 CCTTAGG
Eco81I 5570 CCTGAGG CC^T[ACGT]AGG
6660 CCTTAGG
HpaI 263 GTTAAC GTT^AAC
2202 GTTAAC
NdeI 4132 CATATG CA^TATG
6414 CATATG
PaeR7I 499 CTCGAG C^TCGAG
1544 CTCGAG
PflMI 1704 CCAAACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4736 CCATACAGTGG
PmaCI 6147 CACGTG CAC^GTG
7447 CACGTG
PmlI 6147 CACGTG CAC^GTG
7447 CACGTG
PshBI 5219 ATTAAT AT^TAAT
7723 ATTAAT
Psp1406I 4902 AACGTT AA^CGTT
7521 AACGTT
SmaI 716 CCCGGG CCC^GGG
3300 CCCGGG
SspBI 2259 TGTACA T^GTACA
3421 TGTACA
Van91I 1704 CCAAACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4736 CCATACAGTGG
XbaI 3143 TCTAGA T^CTAGA
5989 TCTAGA
XcmI 4736 CCATACAGTGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5295 CCAAGTCAGTGATGG
XhoI 499 CTCGAG C^TCGAG
1544 CTCGAG
XmaCI 716 CCCGGG C^CCGGG
3300 CCCGGG
XmaI 716 CCCGGG C^CCGGG
3300 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AccII AciI AcsI AfaI AflII
AflIII AluI AlwI AlwNI ApoI
Asp700I AspHI AspI AvaI AvaII
AvrII BamHI BanII BbsI BbvCI
BbvI BcnI BfaI BfrI BlnI
BmyI BpmI BpuAI BsaAI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspHI BspLU11I BspMI BsrBI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DraIII DsaI EarI
EcoNI EcoO109I EcoRI EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflFI PleI PpuMI
PshAI PstI PvuII RcaI RsaI
SacI SapI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
XhoII XmnI