################################## # NO CUT ENZYMES ( 128 enzymes) # ################################## AatII AccI AccII AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BamHI BbrPI BbsI BclI BcnI BfrI BlnI BpuAI BsaAI BsaBI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KspI MaeII MamI MfeI MluI MroI MseI MunI MvnI NaeI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I Tth111I XbaI XcmI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### Acc65I 1156 GGTACC G^GTACC AcsI 365 GAATTC [GA]^AATT[CT] AcyI 324 GGCGCC G[GA]^CG[CT]C ApaI 411 GGGCCC GGGCC^C ApoI 365 GAATTC [GA]^AATT[CT] Asp718I 1156 GGTACC G^GTACC BalI 1075 TGGCCA TGG^CCA BbeI 324 GGCGCC GGCGC^C BbvCI 1247 CCTCAGC CCTCAGC(-5/-2) BcgI 526 GCATGGCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 209 GGATAC GTATCC(6/5) BglI 630 GCCCTGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1369 AGATCT A^GATCT BsaHI 324 GGCGCC G[GA]^CG[CT]C BsgI 481 GTGCAG GTGCAG(16/14) BsmI 37 GCATTC GAATGC(1/-1) BsrBI 1179 CCGCTC CCGCTC(-3/-3) BssSI 32 CTCGTG CACGAG(-5/-1) BstXI 817 CCAGTGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraIII 29 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 1075 TGGCCA [CT]^GGCC[GA] EcoNI 1235 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 365 GAATTC G^AATTC HgaI 96 GCGTC GACGC(5/10) Hin1I 324 GGCGCC G[GA]^CG[CT]C KasI 324 GGCGCC G^GCGCC KpnI 1156 GGTACC GGTAC^C MluNI 1075 TGGCCA TGG^CCA MscI 1075 TGGCCA TGG^CCA MslI 564 CATAAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 324 GGCGCC GG^CGCC NcoI 283 CCATGG C^CATGG PaeR7I 534 CTCGAG C^TCGAG PflMI 986 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SapI 506 GCTCTTC GCTCTTC(1/4) SfaNI 1252 GCATC GCATC(5/9) SfoI 324 GGCGCC GGC^GCC SpeI 1331 ACTAGT A^CTAGT StuI 1258 AGGCCT AGG^CCT Tsp509I 366 AATT ^AATT Van91I 986 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 534 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AfaI 225 GTAC GT^AC 1157 GTAC AspHI 195 GAGCTC G[AT]GC[AT]^C 705 GAGCTC AvaI 294 CCCGAG C^[CT]CG[GA]G 534 CTCGAG BanI 324 GGCGCC G^G[CT][GA]CC 1156 GGTACC BfaI 885 CTAG C^TAG 1332 CTAG BlpI 192 GCTGAGC GC^T[ACGT]AGC 1172 GCTGAGC Bpu1102I 192 GCTGAGC GC^T[ACGT]AGC 1172 GCTGAGC BsaWI 940 TCCGGT [AT]^CCGG[AT] 1060 ACCGGA BsiHKAI 195 GAGCTC G[AT]GC[AT]^C 705 GAGCTC BsoBI 294 CCCGAG C^[CT]CG[GA]G 534 CTCGAG CelII 192 GCTGAGC GC^T[ACGT]AGC 1172 GCTGAGC DsaI 271 CCACGG C^C[GA][CT]GG 283 CCATGG HaeII 324 GGCGCC [GA]GCGC^[CT] 454 AGCGCC MaeI 885 CTAG C^TAG 1332 CTAG RsaI 225 GTAC GT^AC 1157 GTAC SacI 195 GAGCTC GAGCT^C 705 GAGCTC SmlI 534 CTCGAG C^T[CT][GA]AG 708 CTCAAG TaqI 535 TCGA T^CGA 1083 TCGA TthHB8I 535 TCGA T^CGA 1083 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AciI AluI AlwI AlwNI AvaII BanII BbvI BmyI BpmI BsaI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PleI PstI PvuII Sau3AI Sau96I ScrFI SfcI StyI TseI Tsp45I TspRI XhoII