##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII AccIII AclI AcsI AflII
AgeI Aor51HI ApoI AscI AseI
AsnI Asp700I AspI AviII BbeI
BbrPI BfrI BseAI BsiEI BsiWI
BsmBI BsmI BspDI BspEI BspHI
BsrBI BsrFI BssHII Bst1107I BstBI
BstZ17I Cfr10I ClaI CpoI DraI
DrdI EagI EclXI Eco47III Eco52I
EcoRI EcoRV EcoT22I FseI FspI
HindIII HpaI KasI KspI MfeI
MluI MroI MunI NaeI NarI
NgoMIV NheI NotI NruI NsiI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI SwaI Tth111I XbaI
XmnI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
Acc65I 2008 GGTACC G^GTACC
AccI 3079 GTCTAC GT^[AC][GT]AC
AcyI 2248 GGCGTC G[GA]^CG[CT]C
Alw44I 3104 GTGCAC G^TGCAC
ApaLI 3104 GTGCAC G^TGCAC
Asp718I 2008 GGTACC G^GTACC
AvrII 412 CCTAGG C^CTAGG
BcgI 2208 CGAGGTTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1464 TGATCA T^GATCA
BglII 1638 AGATCT A^GATCT
BlnI 412 CCTAGG C^CTAGG
BlpI 43 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 43 GCTGAGC GC^T[ACGT]AGC
BsaAI 4033 TACGTG [CT]AC^GT[GA]
BsaBI 3954 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2248 GGCGTC G[GA]^CG[CT]C
BsaWI 2343 ACCGGA [AT]^CCGG[AT]
BspLU11I 2750 ACATGT A^CATGT
BssSI 4223 CTCGTG CACGAG(-5/-1)
CelII 43 GCTGAGC GC^T[ACGT]AGC
FbaI 1464 TGATCA T^GATCA
HaeII 40 GGCGCT [GA]GCGC^[CT]
Hin1I 2248 GGCGTC G[GA]^CG[CT]C
KpnI 2008 GGTACC GGTAC^C
MamI 3954 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 3182 TTAA T^TAA
NdeI 1982 CATATG CA^TATG
PflMI 3117 CCAGCATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ScaI 1896 AGTACT AGT^ACT
Tru9I 3182 TTAA T^TAA
Van91I 3117 CCAGCATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AflIII 9 ACACGT A^C[GA][CT]GT
2750 ACATGT
AhdI 523 GACCTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1111 GACAGCACGTC
AspEI 523 GACCTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1111 GACAGCACGTC
BalI 424 TGGCCA TGG^CCA
917 TGGCCA
BamHI 76 GGATCC G^GATCC
3427 GGATCC
BciVI 1555 GTATCC GTATCC(6/5)
3176 GTATCC
BsaI 1085 GAGACC GGTCTC(1/5)
3859 GAGACC
BsgI 463 CTGCAC GTGCAG(16/14)
2756 CTGCAC
Bsp1407I 1259 TGTACA T^GTACA
3015 TGTACA
BspMI 3212 ACCTGC ACCTGC(4/8)
3098 GCAGGT
BsrGI 1259 TGTACA T^GTACA
3015 TGTACA
BstAPI 103 GCAGCTATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2926 GCACATTCTGC
DraIII 1934 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
2718 CACATCGTG
Eam1105I 523 GACCTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1111 GACAGCACGTC
HincII 1534 GTTGAC GT[CT]^[GA]AC
2307 GTCAAC
HindII 1534 GTTGAC GT[CT]^[GA]AC
2307 GTCAAC
MluNI 424 TGGCCA TGG^CCA
917 TGGCCA
MscI 424 TGGCCA TGG^CCA
917 TGGCCA
NcoI 144 CCATGG C^CATGG
588 CCATGG
SapI 872 GCTCTTC GCTCTTC(1/4)
1564 GCTCTTC
SspBI 1259 TGTACA T^GTACA
3015 TGTACA
Tsp509I 1632 AATT ^AATT
2534 AATT
XcmI 2717 CCACATCGTGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2993 CCAGGCTACCTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI ApaI AspHI AvaI
AvaII BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrDI BsrI BssKI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PaeR7I PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StuI StyI TaqI TfiI
TseI Tsp45I TspRI TthHB8I XhoI
XhoII XmaCI XmaI