################################## # NO CUT ENZYMES ( 77 enzymes) # ################################## AatII Acc65I AclI AflIII AgeI AhdI AscI AseI AsnI Asp718I AspEI AviII AvrII BbrPI BcgI BclI BglII BlnI BsaAI BsaBI BsiWI BsmBI BspHI BspLU11I BsrDI BssHII Bst1107I BstBI BstEII BstPI BstZ17I CpoI EagI Eam1105I EclXI Eco52I EcoO65I EcoRV FbaI FseI FspI HpaI KpnI MamI MluI NaeI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI Sse8387I SspI SwaI TfiI XbaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccI 1857 GTCTAC GT^[AC][GT]AC AccIII 2081 TCCGGA T^CCGGA AcyI 316 GGCGCC G[GA]^CG[CT]C AflII 2188 CTTAAG C^TTAAG Alw44I 2739 GTGCAC G^TGCAC ApaLI 2739 GTGCAC G^TGCAC BbeI 316 GGCGCC GGCGC^C BbsI 3061 GTCTTC GAAGAC(2/6) BciVI 1952 GTATCC GTATCC(6/5) BfrI 2188 CTTAAG C^TTAAG BglI 213 GCCGCCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 3061 GTCTTC GAAGAC(2/6) BsaHI 316 GGCGCC G[GA]^CG[CT]C BsaI 1285 GAGACC GGTCTC(1/5) BseAI 2081 TCCGGA T^CCGGA Bsp1407I 2954 TGTACA T^GTACA BspDI 2222 ATCGAT AT^CGAT BspEI 2081 TCCGGA T^CCGGA BsrGI 2954 TGTACA T^GTACA BssSI 55 CTCGTG CACGAG(-5/-1) BstXI 1380 CCACCGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ClaI 2222 ATCGAT AT^CGAT DraI 1867 TTTAAA TTT^AAA DraIII 2595 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1848 GACAGAGCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 3376 GAATTC G^AATTC EcoT22I 400 ATGCAT ATGCA^T HgaI 2861 GCGTC GACGC(5/10) Hin1I 316 GGCGCC G[GA]^CG[CT]C HindIII 1178 AAGCTT A^AGCTT KasI 316 GGCGCC G^GCGCC KspI 193 CCGCGG CCGC^GG MfeI 3335 CAATTG C^AATTG MroI 2081 TCCGGA T^CCGGA MunI 3335 CAATTG C^AATTG NarI 316 GGCGCC GG^CGCC NsiI 400 ATGCAT ATGCA^T PaeR7I 249 CTCGAG C^TCGAG PshAI 1481 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 3321 CAGCTG CAG^CTG SacII 193 CCGCGG CCGC^GG SfoI 316 GGCGCC GGC^GCC SpeI 1015 ACTAGT A^CTAGT SphI 2603 GCATGC GCATG^C SspBI 2954 TGTACA T^GTACA XhoI 249 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcsI 1457 AAATTC [GA]^AATT[CT] 3376 GAATTC Aor51HI 105 AGCGCT AGC^GCT 2387 AGCGCT ApoI 1457 AAATTC [GA]^AATT[CT] 3376 GAATTC AspI 2143 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2431 GACTGGGTC BbvCI 646 CCTCAGC CCTCAGC(-5/-2) 1003 GCTGAGG BlpI 268 GCTGAGC GC^T[ACGT]AGC 449 GCTCAGC Bpu1102I 268 GCTGAGC GC^T[ACGT]AGC 449 GCTCAGC BsaWI 722 ACCGGA [AT]^CCGG[AT] 2081 TCCGGA BsmI 2096 GAATGC GAATGC(1/-1) 3405 GAATGC BspMI 899 ACCTGC ACCTGC(4/8) 1288 ACCTGC BsrFI 525 GCCGGT [GA]^CCGG[CT] 1382 ACCGGC CelII 268 GCTGAGC GC^T[ACGT]AGC 449 GCTCAGC Cfr10I 525 GCCGGT [GA]^CCGG[CT] 1382 ACCGGC Eco47III 105 AGCGCT AGC^GCT 2387 AGCGCT HincII 1402 GTTGAC GT[CT]^[GA]AC 1837 GTCAAC HindII 1402 GTTGAC GT[CT]^[GA]AC 1837 GTCAAC MaeII 837 ACGT A^CGT 2546 ACGT MslI 2065 CACTCTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2598 CATGTGCATG PflFI 2143 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2431 GACTGGGTC PpuMI 783 AGGTCCC [GA]G^G[AT]CC[CT] 1156 GGGTCCC Tth111I 2143 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2431 GACTGGGTC XcmI 139 CCACTCCCCGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2078 CCATCCGGAAAATGG ######################################### # 3 OR MORE CUT ENZYMES ( 113 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI AocI ApaI Asp700I AspHI AvaI AvaII BalI BamHI BanI BanII BbvI BcnI BfaI BmyI BpmI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrBI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PstI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StuI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmaCI XmaI XmnI