##################################
# NO CUT ENZYMES (   77 enzymes) #
##################################

AatII       Acc65I      AclI        AflIII      AgeI        
AhdI        AscI        AseI        AsnI        Asp718I     
AspEI       AviII       AvrII       BbrPI       BcgI        
BclI        BglII       BlnI        BsaAI       BsaBI       
BsiWI       BsmBI       BspHI       BspLU11I    BsrDI       
BssHII      Bst1107I    BstBI       BstEII      BstPI       
BstZ17I     CpoI        EagI        Eam1105I    EclXI       
Eco52I      EcoO65I     EcoRV       FbaI        FseI        
FspI        HpaI        KpnI        MamI        MluI        
NaeI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NspV        PacI        PinAI       
PmaCI       PmeI        PmlI        PshBI       Psp1406I    
PvuI        RcaI        RsrII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfuI        
SgrAI       SnaBI       Sse8387I    SspI        SwaI        
TfiI        XbaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccI         1857  GTCTAC               GT^[AC][GT]AC
AccIII       2081  TCCGGA               T^CCGGA
AcyI          316  GGCGCC               G[GA]^CG[CT]C
AflII        2188  CTTAAG               C^TTAAG
Alw44I       2739  GTGCAC               G^TGCAC
ApaLI        2739  GTGCAC               G^TGCAC
BbeI          316  GGCGCC               GGCGC^C
BbsI         3061  GTCTTC               GAAGAC(2/6)
BciVI        1952  GTATCC               GTATCC(6/5)
BfrI         2188  CTTAAG               C^TTAAG
BglI          213  GCCGCCACGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI        3061  GTCTTC               GAAGAC(2/6)
BsaHI         316  GGCGCC               G[GA]^CG[CT]C
BsaI         1285  GAGACC               GGTCTC(1/5)
BseAI        2081  TCCGGA               T^CCGGA
Bsp1407I     2954  TGTACA               T^GTACA
BspDI        2222  ATCGAT               AT^CGAT
BspEI        2081  TCCGGA               T^CCGGA
BsrGI        2954  TGTACA               T^GTACA
BssSI          55  CTCGTG               CACGAG(-5/-1)
BstXI        1380  CCACCGGCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI         2222  ATCGAT               AT^CGAT
DraI         1867  TTTAAA               TTT^AAA
DraIII       2595  CACCATGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1848  GACAGAGCGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI        3376  GAATTC               G^AATTC
EcoT22I       400  ATGCAT               ATGCA^T
HgaI         2861  GCGTC                GACGC(5/10)
Hin1I         316  GGCGCC               G[GA]^CG[CT]C
HindIII      1178  AAGCTT               A^AGCTT
KasI          316  GGCGCC               G^GCGCC
KspI          193  CCGCGG               CCGC^GG
MfeI         3335  CAATTG               C^AATTG
MroI         2081  TCCGGA               T^CCGGA
MunI         3335  CAATTG               C^AATTG
NarI          316  GGCGCC               GG^CGCC
NsiI          400  ATGCAT               ATGCA^T
PaeR7I        249  CTCGAG               C^TCGAG
PshAI        1481  GACAGGAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII        3321  CAGCTG               CAG^CTG
SacII         193  CCGCGG               CCGC^GG
SfoI          316  GGCGCC               GGC^GCC
SpeI         1015  ACTAGT               A^CTAGT
SphI         2603  GCATGC               GCATG^C
SspBI        2954  TGTACA               T^GTACA
XhoI          249  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AcsI         1457  AAATTC               [GA]^AATT[CT]
             3376  GAATTC              
Aor51HI       105  AGCGCT               AGC^GCT
             2387  AGCGCT              
ApoI         1457  AAATTC               [GA]^AATT[CT]
             3376  GAATTC              
AspI         2143  GACATTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2431  GACTGGGTC           
BbvCI         646  CCTCAGC              CCTCAGC(-5/-2)
             1003  GCTGAGG             
BlpI          268  GCTGAGC              GC^T[ACGT]AGC
              449  GCTCAGC             
Bpu1102I      268  GCTGAGC              GC^T[ACGT]AGC
              449  GCTCAGC             
BsaWI         722  ACCGGA               [AT]^CCGG[AT]
             2081  TCCGGA              
BsmI         2096  GAATGC               GAATGC(1/-1)
             3405  GAATGC              
BspMI         899  ACCTGC               ACCTGC(4/8)
             1288  ACCTGC              
BsrFI         525  GCCGGT               [GA]^CCGG[CT]
             1382  ACCGGC              
CelII         268  GCTGAGC              GC^T[ACGT]AGC
              449  GCTCAGC             
Cfr10I        525  GCCGGT               [GA]^CCGG[CT]
             1382  ACCGGC              
Eco47III      105  AGCGCT               AGC^GCT
             2387  AGCGCT              
HincII       1402  GTTGAC               GT[CT]^[GA]AC
             1837  GTCAAC              
HindII       1402  GTTGAC               GT[CT]^[GA]AC
             1837  GTCAAC              
MaeII         837  ACGT                 A^CGT
             2546  ACGT                
MslI         2065  CACTCTAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2598  CATGTGCATG          
PflFI        2143  GACATTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2431  GACTGGGTC           
PpuMI         783  AGGTCCC              [GA]G^G[AT]CC[CT]
             1156  GGGTCCC             
Tth111I      2143  GACATTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2431  GACTGGGTC           
XcmI          139  CCACTCCCCGCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2078  CCATCCGGAAAATGG     


#########################################
# 3 OR MORE CUT ENZYMES (  113 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AlwNI       AocI        ApaI        Asp700I     AspHI       
AvaI        AvaII       BalI        BamHI       BanI        
BanII       BbvI        BcnI        BfaI        BmyI        
BpmI        BsaJI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BsrBI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstUI       BstYI       
Bsu36I      Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
Eco81I      EcoNI       EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PleI        PstI        
RsaI        SacI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmaI        SmlI        StuI        
StyI        TaqI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XhoII       
XmaCI       XmaI        XmnI