################################## # NO CUT ENZYMES ( 82 enzymes) # ################################## Acc65I AccII AccIII AclI AgeI AhdI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BcgI BlnI BsaWI BseAI BsiWI BspDI BspEI BspHI BsrBI BssHII Bst1107I BstEII BstPI BstUI BstZ17I ClaI CpoI DraIII Eam1105I Eco47III EcoNI EcoO65I EcoT22I FseI FspI HincII HindII HpaI KpnI KspI MfeI MluI MroI MunI MvnI NheI NotI NruI NsiI PacI PflFI PinAI PmeI PshAI PshBI Psp1406I PstI RcaI RsrII SacII SalI SapI SbfI SfcI SfiI SgrAI SmaI SnaBI SpeI SphI Sse8387I SwaI Tth111I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AatII 2385 GACGTC GACGT^C AccI 270 GTCTAC GT^[AC][GT]AC AlwNI 2459 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1549 CCTGAGG CC^T[ACGT]AGG BbeI 38 GGCGCC GGCGC^C BbrPI 3536 CACGTG CAC^GTG BbvCI 1280 CCTCAGC CCTCAGC(-5/-2) BciVI 3303 GGATAC GTATCC(6/5) BclI 1745 TGATCA T^GATCA BglI 30 GCCATGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 3280 AGATCT A^GATCT BsaAI 3536 CACGTG [CT]AC^GT[GA] BsaBI 1912 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmBI 1203 GAGACG CGTCTC(1/5) BsmFI 2823 GGGAC GGGAC(10/14) BspLU11I 2433 ACATGT A^CATGT BsrFI 569 GCCGGC [GA]^CCGG[CT] BssSI 1100 CTCGTG CACGAG(-5/-1) BstAPI 3172 GCAACACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 327 CCAGGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 3280 AGATCT [GA]^GATC[CT] Bsu36I 1549 CCTGAGG CC^T[ACGT]AGG Cfr10I 569 GCCGGC [GA]^CCGG[CT] DrdI 83 GACTGCGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 31 CCATGG C^C[GA][CT]GG EagI 571 CGGCCG C^GGCCG EclXI 571 CGGCCG C^GGCCG Eco52I 571 CGGCCG C^GGCCG Eco81I 1549 CCTGAGG CC^T[ACGT]AGG EcoRI 468 GAATTC G^AATTC FbaI 1745 TGATCA T^GATCA HaeII 38 GGCGCC [GA]GCGC^[CT] HindIII 2677 AAGCTT A^AGCTT KasI 38 GGCGCC G^GCGCC MamI 1912 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 3280 AGATCT [GA]^GATC[CT] NaeI 569 GCCGGC GCC^GGC NarI 38 GGCGCC GG^CGCC NcoI 31 CCATGG C^CATGG NgoMIV 569 GCCGGC G^CCGGC NspI 2433 ACATGT [GA]CATG^[CT] PflMI 1810 CCAGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 3536 CACGTG CAC^GTG PmlI 3536 CACGTG CAC^GTG PvuI 3331 CGATCG CGAT^CG ScaI 1180 AGTACT AGT^ACT SexAI 599 ACCTGGT A^CC[AT]GGT SfoI 38 GGCGCC GGC^GCC StuI 359 AGGCCT AGG^CCT Van91I 1810 CCAGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 3280 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### Alw44I 536 GTGCAC G^TGCAC 987 GTGCAC AlwI 2488 GATCC GGATC(4/5) 1951 GGATC ApaLI 536 GTGCAC G^TGCAC 987 GTGCAC Asp700I 346 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3555 GAATTGCTTC BalI 1478 TGGCCA TGG^CCA 3767 TGGCCA BanI 38 GGCGCC G^G[CT][GA]CC 1503 GGTGCC BbvI 16 GCAGC GCAGC(8/12) 835 GCAGC BcnI 1268 CCCGG CC^[GC]GG 2228 CCCGG BpmI 1236 CTCCAG CTGGAG(16/14) 1397 CTCCAG BsaI 1219 GAGACC GGTCTC(1/5) 3812 GGTCTC BsmI 1085 GCATTC GAATGC(1/-1) 2584 GCATTC Bsp1407I 727 TGTACA T^GTACA 3572 TGTACA BsrDI 815 GCAATG GCAATG(2/0) 3167 GCAATG BsrGI 727 TGTACA T^GTACA 3572 TGTACA BstBI 683 TTCGAA TT^CGAA 1033 TTCGAA DraII 1022 AGGACCT [GA]G^G[ACGT]CC[CT] 2823 GGGACCT EcoO109I 1022 AGGACCT [GA]G^G[ACGT]CC[CT] 2823 GGGACCT EcoRV 1318 GATATC GAT^ATC 1585 GATATC FauI 2236 CCCGC CCCGC(4/6) 133 GCGGG MluNI 1478 TGGCCA TGG^CCA 3767 TGGCCA MscI 1478 TGGCCA TGG^CCA 3767 TGGCCA NciI 1268 CCCGG CC^[GC]GG 2228 CCCGG NdeI 667 CATATG CA^TATG 1600 CATATG NspV 683 TTCGAA TT^CGAA 1033 TTCGAA PaeR7I 25 CTCGAG C^TCGAG 2167 CTCGAG PpuMI 1022 AGGACCT [GA]G^G[AT]CC[CT] 2823 GGGACCT PvuII 1283 CAGCTG CAG^CTG 1907 CAGCTG SacI 1147 GAGCTC GAGCT^C 1441 GAGCTC SfuI 683 TTCGAA TT^CGAA 1033 TTCGAA SspBI 727 TGTACA T^GTACA 3572 TGTACA SspI 3725 AATATT AAT^ATT 3775 AATATT TseI 16 GCAGC G^C[AT]GC 835 GCAGC XcmI 1421 CCAGGTGGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2401 CCAAAGGAGAGCTGG XhoI 25 CTCGAG C^TCGAG 2167 CTCGAG XmnI 346 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3555 GAATTGCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 90 enzymes) # ######################################### AciI AcsI AcyI AfaI AflII AflIII AluI ApoI AspHI AvaI AvaII BanII BbsI BfaI BfrI BlpI BmyI Bpu1102I BpuAI BsaHI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Cac8I CelII CfoI Cfr13I DdeI DpnII DraI EaeI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SmlI StyI TaqI TfiI Tru9I Tsp45I Tsp509I TspRI TthHB8I XbaI