##################################
# NO CUT ENZYMES (   76 enzymes) #
##################################

AatII       Acc65I      AccIII      AcyI        AgeI        
AhdI        AscI        Asp718I     AspEI       AspI        
AvrII       BbeI        BbvCI       BcgI        BciVI       
BcnI        BlnI        BlpI        Bpu1102I    BsaHI       
BsaI        BsaWI       BseAI       BsiEI       BsiWI       
BspDI       BspEI       BsrBI       BssHII      BssSI       
BstBI       CelII       ClaI        CpoI        DrdI        
EagI        Eam1105I    EclXI       Eco52I      EcoRI       
FseI        Hin1I       KasI        KpnI        KspI        
MluI        MroI        NarI        NciI        NcoI        
NheI        NotI        NruI        NspV        PaeR7I      
PflFI       PinAI       PmeI        PpuMI       PvuI        
RsrII       SacII       SalI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        Tth111I     XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AclI         7032  AACGTT               AA^CGTT
AflII         707  CTTAAG               C^TTAAG
Alw44I       6390  GTGCAC               G^TGCAC
Aor51HI      3025  AGCGCT               AGC^GCT
ApaI         3778  GGGCCC               GGGCC^C
ApaLI        6390  GTGCAC               G^TGCAC
AvaI         6032  CCCGAG               C^[CT]CG[GA]G
AviII        2869  TGCGCA               TGC^GCA
BanI         4850  GGTGCC               G^G[CT][GA]CC
BbrPI        7116  CACGTG               CAC^GTG
BfrI          707  CTTAAG               C^TTAAG
BglI         4875  GCCCTGCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        7587  AGATCT               A^GATCT
BsaBI         553  GATTAAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI        4929  CGTCTC               CGTCTC(1/5)
BsoBI        6032  CCCGAG               C^[CT]CG[GA]G
BsrFI        3795  GCCGGC               [GA]^CCGG[CT]
Bst1107I     2757  GTATAC               GTA^TAC
BstXI        3244  CCAAAGGGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      2757  GTATAC               GTA^TAC
Cfr10I       3795  GCCGGC               [GA]^CCGG[CT]
Eco47III     3025  AGCGCT               AGC^GCT
EcoNI        1772  CCTCTTAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI         1568  GCGGG                CCCGC(4/6)
FspI         2869  TGCGCA               TGC^GCA
HaeII        3025  AGCGCT               [GA]GCGC^[CT]
HapII        3796  CCGG                 C^CGG
HpaI         6378  GTTAAC               GTT^AAC
HpaII        3796  CCGG                 C^CGG
MamI          553  GATTAAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         7081  CAATTG               C^AATTG
MspI         3796  CCGG                 C^CGG
MunI         7081  CAATTG               C^AATTG
NaeI         3795  GCCGGC               GCC^GGC
NgoMIV       3795  GCCGGC               G^CCGGC
PacI         4424  TTAATTAA             TTAAT^TAA
PmaCI        7116  CACGTG               CAC^GTG
PmlI         7116  CACGTG               CAC^GTG
PshAI        2065  GACTAAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     7032  AACGTT               AA^CGTT
SbfI         4877  CCTGCAGG             CCTGCA^GG
ScaI         6075  AGTACT               AGT^ACT
SphI         5934  GCATGC               GCATG^C
Sse8387I     4877  CCTGCAGG             CCTGCA^GG
StuI         6898  AGGCCT               AGG^CCT
TaqI         1998  TCGA                 T^CGA
TthHB8I      1998  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AocI         6894  CCTCAGG              CC^T[ACGT]AGG
             7534  CCTTAGG             
BamHI         280  GGATCC               G^GATCC
             5637  GGATCC              
BclI          271  TGATCA               T^GATCA
              307  TGATCA              
Bsp1407I     2501  TGTACA               T^GTACA
             6511  TGTACA              
BspLU11I     4296  ACATGT               A^CATGT
             6948  ACATGT              
BspMI        4871  ACCTGC               ACCTGC(4/8)
             4107  GCAGGT              
BsrGI        2501  TGTACA               T^GTACA
             6511  TGTACA              
Bsu36I       6894  CCTCAGG              CC^T[ACGT]AGG
             7534  CCTTAGG             
DraII        3777  GGGGCCC              [GA]G^G[ACGT]CC[CT]
             3778  GGGCCCT             
DraIII       5346  CACACCGTG            CAC[ACGT][ACGT][ACGT]^GTG
             5543  CACCGAGTG           
Eco81I       6894  CCTCAGG              CC^T[ACGT]AGG
             7534  CCTTAGG             
EcoO109I     3777  GGGGCCC              [GA]G^G[ACGT]CC[CT]
             3778  GGGCCCT             
EcoRV        7373  GATATC               GAT^ATC
             7678  GATATC              
EcoT14I       761  CCAAGG               C^C[AT][AT]GG
             5013  CCTTGG              
FbaI          271  TGATCA               T^GATCA
              307  TGATCA              
HgaI         7121  GACGC                GACGC(5/10)
             2989  GCGTC               
MspA1I         49  CAGCTG               C[AC]G^C[GT]G
             2189  CAGCTG              
NdeI           10  CATATG               CA^TATG
             1935  CATATG              
PvuII          49  CAGCTG               CAG^CTG
             2189  CAGCTG              
SacI         2277  GAGCTC               GAGCT^C
             6184  GAGCTC              
SspBI        2501  TGTACA               T^GTACA
             6511  TGTACA              
StyI          761  CCAAGG               C^C[AT][AT]GG
             5013  CCTTGG              
SwaI          512  ATTTAAAT             ATTT^AAAT
             3508  ATTTAAAT            
XbaI         5830  TCTAGA               T^CTAGA
             7369  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (  111 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AfaI        
AflIII      AluI        AlwI        AlwNI       ApoI        
AseI        AsnI        Asp700I     AspHI       AvaII       
BalI        BanII       BbsI        BbvI        BfaI        
BmyI        BpmI        BpuAI       BsaAI       BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsmI        Bsp1286I    BspHI       
BsrDI       BsrI        BssKI       BstAPI      BstEII      
BstF5I      BstNI       BstPI       BstUI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraI        DsaI        EaeI        EarI        EcoO65I     
EcoRII      EcoT22I     Fnu4HI      FokI        HaeIII      
HhaI        HinP1I      HincII      HindII      HindIII     
HinfI       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MslI        
MvaI        MvnI        MwoI        NdeII       NlaIII      
NlaIV       NsiI        NspI        PflMI       PleI        
PshBI       PstI        RcaI        RsaI        SapI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        SspI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       Van91I      XhoII       
XmnI