################################## # NO CUT ENZYMES ( 76 enzymes) # ################################## AatII Acc65I AccIII AcyI AgeI AhdI AscI Asp718I AspEI AspI AvrII BbeI BbvCI BcgI BciVI BcnI BlnI BlpI Bpu1102I BsaHI BsaI BsaWI BseAI BsiEI BsiWI BspDI BspEI BsrBI BssHII BssSI BstBI CelII ClaI CpoI DrdI EagI Eam1105I EclXI Eco52I EcoRI FseI Hin1I KasI KpnI KspI MluI MroI NarI NciI NcoI NheI NotI NruI NspV PaeR7I PflFI PinAI PmeI PpuMI PvuI RsrII SacII SalI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Tth111I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AclI 7032 AACGTT AA^CGTT AflII 707 CTTAAG C^TTAAG Alw44I 6390 GTGCAC G^TGCAC Aor51HI 3025 AGCGCT AGC^GCT ApaI 3778 GGGCCC GGGCC^C ApaLI 6390 GTGCAC G^TGCAC AvaI 6032 CCCGAG C^[CT]CG[GA]G AviII 2869 TGCGCA TGC^GCA BanI 4850 GGTGCC G^G[CT][GA]CC BbrPI 7116 CACGTG CAC^GTG BfrI 707 CTTAAG C^TTAAG BglI 4875 GCCCTGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 7587 AGATCT A^GATCT BsaBI 553 GATTAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmBI 4929 CGTCTC CGTCTC(1/5) BsoBI 6032 CCCGAG C^[CT]CG[GA]G BsrFI 3795 GCCGGC [GA]^CCGG[CT] Bst1107I 2757 GTATAC GTA^TAC BstXI 3244 CCAAAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 2757 GTATAC GTA^TAC Cfr10I 3795 GCCGGC [GA]^CCGG[CT] Eco47III 3025 AGCGCT AGC^GCT EcoNI 1772 CCTCTTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FauI 1568 GCGGG CCCGC(4/6) FspI 2869 TGCGCA TGC^GCA HaeII 3025 AGCGCT [GA]GCGC^[CT] HapII 3796 CCGG C^CGG HpaI 6378 GTTAAC GTT^AAC HpaII 3796 CCGG C^CGG MamI 553 GATTAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 7081 CAATTG C^AATTG MspI 3796 CCGG C^CGG MunI 7081 CAATTG C^AATTG NaeI 3795 GCCGGC GCC^GGC NgoMIV 3795 GCCGGC G^CCGGC PacI 4424 TTAATTAA TTAAT^TAA PmaCI 7116 CACGTG CAC^GTG PmlI 7116 CACGTG CAC^GTG PshAI 2065 GACTAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 7032 AACGTT AA^CGTT SbfI 4877 CCTGCAGG CCTGCA^GG ScaI 6075 AGTACT AGT^ACT SphI 5934 GCATGC GCATG^C Sse8387I 4877 CCTGCAGG CCTGCA^GG StuI 6898 AGGCCT AGG^CCT TaqI 1998 TCGA T^CGA TthHB8I 1998 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AocI 6894 CCTCAGG CC^T[ACGT]AGG 7534 CCTTAGG BamHI 280 GGATCC G^GATCC 5637 GGATCC BclI 271 TGATCA T^GATCA 307 TGATCA Bsp1407I 2501 TGTACA T^GTACA 6511 TGTACA BspLU11I 4296 ACATGT A^CATGT 6948 ACATGT BspMI 4871 ACCTGC ACCTGC(4/8) 4107 GCAGGT BsrGI 2501 TGTACA T^GTACA 6511 TGTACA Bsu36I 6894 CCTCAGG CC^T[ACGT]AGG 7534 CCTTAGG DraII 3777 GGGGCCC [GA]G^G[ACGT]CC[CT] 3778 GGGCCCT DraIII 5346 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG 5543 CACCGAGTG Eco81I 6894 CCTCAGG CC^T[ACGT]AGG 7534 CCTTAGG EcoO109I 3777 GGGGCCC [GA]G^G[ACGT]CC[CT] 3778 GGGCCCT EcoRV 7373 GATATC GAT^ATC 7678 GATATC EcoT14I 761 CCAAGG C^C[AT][AT]GG 5013 CCTTGG FbaI 271 TGATCA T^GATCA 307 TGATCA HgaI 7121 GACGC GACGC(5/10) 2989 GCGTC MspA1I 49 CAGCTG C[AC]G^C[GT]G 2189 CAGCTG NdeI 10 CATATG CA^TATG 1935 CATATG PvuII 49 CAGCTG CAG^CTG 2189 CAGCTG SacI 2277 GAGCTC GAGCT^C 6184 GAGCTC SspBI 2501 TGTACA T^GTACA 6511 TGTACA StyI 761 CCAAGG C^C[AT][AT]GG 5013 CCTTGG SwaI 512 ATTTAAAT ATTT^AAAT 3508 ATTTAAAT XbaI 5830 TCTAGA T^CTAGA 7369 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 111 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AseI AsnI Asp700I AspHI AvaII BalI BanII BbsI BbvI BfaI BmyI BpmI BpuAI BsaAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspHI BsrDI BsrI BssKI BstAPI BstEII BstF5I BstNI BstPI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DsaI EaeI EarI EcoO65I EcoRII EcoT22I Fnu4HI FokI HaeIII HhaI HinP1I HincII HindII HindIII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MvaI MvnI MwoI NdeII NlaIII NlaIV NsiI NspI PflMI PleI PshBI PstI RcaI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XhoII XmnI