################################## # NO CUT ENZYMES ( 70 enzymes) # ################################## AccII AccIII AclI AflIII AgeI AhdI Aor51HI ApaI AscI AspEI BbeI BbrPI BseAI BsiEI BsiWI BsmBI BspEI BspLU11I BsrFI BssHII BstBI BstUI Cfr10I CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI FseI HaeII HpaI KasI KspI MfeI MluI MroI MunI MvnI NaeI NarI NgoMIV NheI NotI NruI NspV PaeR7I PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AatII 6966 GACGTC GACGT^C Acc65I 2885 GGTACC G^GTACC Alw44I 6483 GTGCAC G^TGCAC ApaLI 6483 GTGCAC G^TGCAC Asp718I 2885 GGTACC G^GTACC AvrII 939 CCTAGG C^CTAGG BanI 2885 GGTACC G^G[CT][GA]CC BcgI 8484 CGAGTCCGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 7045 GGATAC GTATCC(6/5) BcnI 7490 CCCGG CC^[GC]GG BglI 1786 GCCTCTAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 7972 AGATCT A^GATCT BlnI 939 CCTAGG C^CTAGG BsaAI 3886 CACGTA [CT]AC^GT[GA] BsaBI 6217 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BspDI 910 ATCGAT AT^CGAT Bst1107I 4871 GTATAC GTA^TAC BstEII 7163 GGTCACC G^GT[ACGT]ACC BstPI 7163 GGTCACC G^GT[ACGT]ACC BstXI 5651 CCAGAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 4871 GTATAC GTA^TAC ClaI 910 ATCGAT AT^CGAT DraIII 7634 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO65I 7163 GGTCACC G^GT[ACGT]ACC EcoRV 2092 GATATC GAT^ATC FauI 1613 GCGGG CCCGC(4/6) KpnI 2885 GGTACC GGTAC^C MamI 6217 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NciI 7490 CCCGG CC^[GC]GG PshAI 7644 GACTCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SexAI 7964 ACCTGGT A^CC[AT]GGT SpeI 3214 ACTAGT A^CTAGT StuI 4809 AGGCCT AGG^CCT ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AcyI 3771 GGCGTC G[GA]^CG[CT]C 6966 GACGTC AocI 4774 CCTGAGG CC^T[ACGT]AGG 7501 CCTCAGG AseI 1023 ATTAAT AT^TAAT 1445 ATTAAT AsnI 1023 ATTAAT AT^TAAT 1445 ATTAAT AvaI 6042 CCCGAG C^[CT]CG[GA]G 8482 CCCGAG AviII 3752 TGCGCA TGC^GCA 3852 TGCGCA BamHI 2420 GGATCC G^GATCC 4262 GGATCC BbvCI 1781 CCTCAGC CCTCAGC(-5/-2) 6751 CCTCAGC BclI 5072 TGATCA T^GATCA 8275 TGATCA BlpI 7009 GCTCAGC GC^T[ACGT]AGC 7095 GCTGAGC Bpu1102I 7009 GCTCAGC GC^T[ACGT]AGC 7095 GCTGAGC BsaHI 3771 GGCGTC G[GA]^CG[CT]C 6966 GACGTC BsaWI 2121 ACCGGA [AT]^CCGG[AT] 8488 TCCGGT BsoBI 6042 CCCGAG C^[CT]CG[GA]G 8482 CCCGAG BspMI 1134 ACCTGC ACCTGC(4/8) 8002 ACCTGC BsrBI 7596 CCGCTC CCGCTC(-3/-3) 1611 GAGCGG BsrDI 4306 CATTGC GCAATG(2/0) 7689 CATTGC BssSI 3840 CTCGTG CACGAG(-5/-1) 6020 CTCGTG BstAPI 203 GCAGACTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 6508 GCATCTTATGC Bsu36I 4774 CCTGAGG CC^T[ACGT]AGG 7501 CCTCAGG CelII 7009 GCTCAGC GC^T[ACGT]AGC 7095 GCTGAGC Eco81I 4774 CCTGAGG CC^T[ACGT]AGG 7501 CCTCAGG EcoRI 2279 GAATTC G^AATTC 7231 GAATTC FbaI 5072 TGATCA T^GATCA 8275 TGATCA FspI 3752 TGCGCA TGC^GCA 3852 TGCGCA HgaI 7153 GACGC GACGC(5/10) 3772 GCGTC Hin1I 3771 GGCGTC G[GA]^CG[CT]C 6966 GACGTC HincII 5379 GTCAAC GT[CT]^[GA]AC 8507 GTTGAC HindII 5379 GTCAAC GT[CT]^[GA]AC 8507 GTTGAC HindIII 3315 AAGCTT A^AGCTT 6289 AAGCTT PacI 3319 TTAATTAA TTAAT^TAA 8590 TTAATTAA PpuMI 1979 AGGACCC [GA]G^G[AT]CC[CT] 2896 GGGTCCT PshBI 1023 ATTAAT AT^TAAT 1445 ATTAAT SacI 2752 GAGCTC GAGCT^C 7456 GAGCTC SapI 1120 GCTCTTC GCTCTTC(1/4) 4046 GCTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 120 enzymes) # ######################################### AccI AciI AcsI AfaI AflII AluI AlwI AlwNI ApoI Asp700I AspHI AspI AvaII BalI BanII BbsI BbvI BfaI BfrI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BspHI BsrGI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I EcoT22I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NcoI NdeI NdeII NlaIII NlaIV NsiI NspI PflFI PflMI PleI PstI PvuII RcaI RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SphI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoII XmnI