##################################
# NO CUT ENZYMES (   47 enzymes) #
##################################

AatII       Acc65I      AclI        AgeI        AhdI        
AscI        AseI        AsnI        Asp718I     AspEI       
BbrPI       BsaBI       BsiEI       BsiWI       BsmBI       
BssHII      BstBI       CpoI        DraI        EagI        
Eam1105I    EclXI       Eco52I      EcoRV       FseI        
KpnI        MamI        NaeI        NdeI        NgoMIV      
NheI        NotI        NruI        NspV        PinAI       
PmaCI       PmeI        PmlI        PshBI       Psp1406I    
PvuI        RsrII       SalI        SfuI        SgrAI       
SspI        SwaI        


###################################
# ONE CUT ENZYMES (   23 enzymes) #
###################################

ApaI         4383  GGGCCC               GGGCC^C
BamHI        4684  GGATCC               G^GATCC
BbeI         5616  GGCGCC               GGCGC^C
BclI         3625  TGATCA               T^GATCA
BsaAI        1759  TACGTA               [CT]AC^GT[GA]
BspDI        6779  ATCGAT               AT^CGAT
Bst1107I      531  GTATAC               GTA^TAC
BstZ17I       531  GTATAC               GTA^TAC
ClaI         6779  ATCGAT               AT^CGAT
FbaI         3625  TGATCA               T^GATCA
HindIII      5355  AAGCTT               A^AGCTT
HpaI         9200  GTTAAC               GTT^AAC
KasI         5616  GGCGCC               G^GCGCC
MfeI         6392  CAATTG               C^AATTG
MluI         1056  ACGCGT               A^CGCGT
MunI         6392  CAATTG               C^AATTG
NarI         5616  GGCGCC               GG^CGCC
PacI         8877  TTAATTAA             TTAAT^TAA
SfiI         4045  GGCCATGGTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI         5616  GGCGCC               GGC^GCC
SnaBI        1759  TACGTA               TAC^GTA
SpeI         2111  ACTAGT               A^CTAGT
XbaI         3247  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AccI          531  GTATAC               GT^[AC][GT]AC
             7915  GTAGAC              
AspI         5371  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             6422  GACGTGGTC           
AviII        5865  TGCGCA               TGC^GCA
             7973  TGCGCA              
BcgI         1888  CGAGGACTATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             2137  CGATGAGTTTGC        
BglII        6999  AGATCT               A^GATCT
             8358  AGATCT              
BspHI        6033  TCATGA               T^CATGA
             6561  TCATGA              
BspLU11I     7532  ACATGT               A^CATGT
             9279  ACATGT              
BsrFI        2370  ACCGGC               [GA]^CCGG[CT]
             2547  GCCGGT              
BstEII       5951  GGTCACC              G^GT[ACGT]ACC
             7162  GGTCACC             
BstPI        5951  GGTCACC              G^GT[ACGT]ACC
             7162  GGTCACC             
Cfr10I       2370  ACCGGC               [GA]^CCGG[CT]
             2547  GCCGGT              
DrdI         3134  GACCAGGTTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4598  GACATGGCTGTC        
EcoO65I      5951  GGTCACC              G^GT[ACGT]ACC
             7162  GGTCACC             
EcoRI        1184  GAATTC               G^AATTC
             2773  GAATTC              
EcoT22I         7  ATGCAT               ATGCA^T
             4509  ATGCAT              
FspI         5865  TGCGCA               TGC^GCA
             7973  TGCGCA              
KspI         2847  CCGCGG               CCGC^GG
             5403  CCGCGG              
NsiI            7  ATGCAT               ATGCA^T
             4509  ATGCAT              
PflFI        5371  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             6422  GACGTGGTC           
RcaI         6033  TCATGA               T^CATGA
             6561  TCATGA              
SacII        2847  CCGCGG               CCGC^GG
             5403  CCGCGG              
SbfI          849  CCTGCAGG             CCTGCA^GG
              871  CCTGCAGG            
SexAI        3135  ACCAGGT              A^CC[AT]GGT
             9195  ACCAGGT             
SmaI          746  CCCGGG               CCC^GGG
             2427  CCCGGG              
Sse8387I      849  CCTGCAGG             CCTGCA^GG
              871  CCTGCAGG            
Tth111I      5371  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             6422  GACGTGGTC           
XmaCI         746  CCCGGG               C^CCGGG
             2427  CCCGGG              
XmaI          746  CCCGGG               C^CCGGG
             2427  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  160 enzymes) #
#########################################

AccII       AccIII      AciI        AcsI        AcyI        
AfaI        AflII       AflIII      AluI        Alw44I      
AlwI        AlwNI       AocI        Aor51HI     ApaLI       
ApoI        Asp700I     AspHI       AvaI        AvaII       
AvrII       BalI        BanI        BanII       BbsI        
BbvCI       BbvI        BciVI       BcnI        BfaI        
BfrI        BglI        BlnI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaHI       BsaI        
BsaJI       BsaWI       BseAI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsmI        BsoBI       Bsp1286I    Bsp1407I    BspEI       
BspMI       BsrBI       BsrDI       BsrGI       BsrI        
BssKI       BssSI       BstAPI      BstF5I      BstNI       
BstUI       BstXI       BstYI       Bsu36I      Cac8I       
CelII       CfoI        Cfr13I      DdeI        DpnII       
DraII       DraIII      DsaI        EaeI        EarI        
Eco47III    Eco81I      EcoNI       EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MroI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
NspI        PaeR7I      PflMI       PleI        PpuMI       
PshAI       PstI        PvuII       RsaI        SacI        
SapI        Sau3AI      Sau96I      ScaI        ScrFI       
SfaNI       SfcI        SmlI        SphI        SspBI       
StuI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XcmI        XhoI        XhoII       XmnI