################################## # NO CUT ENZYMES ( 47 enzymes) # ################################## AatII Acc65I AclI AgeI AhdI AscI AseI AsnI Asp718I AspEI BbrPI BsaBI BsiEI BsiWI BsmBI BssHII BstBI CpoI DraI EagI Eam1105I EclXI Eco52I EcoRV FseI KpnI MamI NaeI NdeI NgoMIV NheI NotI NruI NspV PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SalI SfuI SgrAI SspI SwaI ################################### # ONE CUT ENZYMES ( 23 enzymes) # ################################### ApaI 4383 GGGCCC GGGCC^C BamHI 4684 GGATCC G^GATCC BbeI 5616 GGCGCC GGCGC^C BclI 3625 TGATCA T^GATCA BsaAI 1759 TACGTA [CT]AC^GT[GA] BspDI 6779 ATCGAT AT^CGAT Bst1107I 531 GTATAC GTA^TAC BstZ17I 531 GTATAC GTA^TAC ClaI 6779 ATCGAT AT^CGAT FbaI 3625 TGATCA T^GATCA HindIII 5355 AAGCTT A^AGCTT HpaI 9200 GTTAAC GTT^AAC KasI 5616 GGCGCC G^GCGCC MfeI 6392 CAATTG C^AATTG MluI 1056 ACGCGT A^CGCGT MunI 6392 CAATTG C^AATTG NarI 5616 GGCGCC GG^CGCC PacI 8877 TTAATTAA TTAAT^TAA SfiI 4045 GGCCATGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 5616 GGCGCC GGC^GCC SnaBI 1759 TACGTA TAC^GTA SpeI 2111 ACTAGT A^CTAGT XbaI 3247 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccI 531 GTATAC GT^[AC][GT]AC 7915 GTAGAC AspI 5371 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 6422 GACGTGGTC AviII 5865 TGCGCA TGC^GCA 7973 TGCGCA BcgI 1888 CGAGGACTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2137 CGATGAGTTTGC BglII 6999 AGATCT A^GATCT 8358 AGATCT BspHI 6033 TCATGA T^CATGA 6561 TCATGA BspLU11I 7532 ACATGT A^CATGT 9279 ACATGT BsrFI 2370 ACCGGC [GA]^CCGG[CT] 2547 GCCGGT BstEII 5951 GGTCACC G^GT[ACGT]ACC 7162 GGTCACC BstPI 5951 GGTCACC G^GT[ACGT]ACC 7162 GGTCACC Cfr10I 2370 ACCGGC [GA]^CCGG[CT] 2547 GCCGGT DrdI 3134 GACCAGGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4598 GACATGGCTGTC EcoO65I 5951 GGTCACC G^GT[ACGT]ACC 7162 GGTCACC EcoRI 1184 GAATTC G^AATTC 2773 GAATTC EcoT22I 7 ATGCAT ATGCA^T 4509 ATGCAT FspI 5865 TGCGCA TGC^GCA 7973 TGCGCA KspI 2847 CCGCGG CCGC^GG 5403 CCGCGG NsiI 7 ATGCAT ATGCA^T 4509 ATGCAT PflFI 5371 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 6422 GACGTGGTC RcaI 6033 TCATGA T^CATGA 6561 TCATGA SacII 2847 CCGCGG CCGC^GG 5403 CCGCGG SbfI 849 CCTGCAGG CCTGCA^GG 871 CCTGCAGG SexAI 3135 ACCAGGT A^CC[AT]GGT 9195 ACCAGGT SmaI 746 CCCGGG CCC^GGG 2427 CCCGGG Sse8387I 849 CCTGCAGG CCTGCA^GG 871 CCTGCAGG Tth111I 5371 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 6422 GACGTGGTC XmaCI 746 CCCGGG C^CCGGG 2427 CCCGGG XmaI 746 CCCGGG C^CCGGG 2427 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 160 enzymes) # ######################################### AccII AccIII AciI AcsI AcyI AfaI AflII AflIII AluI Alw44I AlwI AlwNI AocI Aor51HI ApaLI ApoI Asp700I AspHI AvaI AvaII AvrII BalI BanI BanII BbsI BbvCI BbvI BciVI BcnI BfaI BfrI BglI BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspEI BspMI BsrBI BsrDI BsrGI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco47III Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MroI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PaeR7I PflMI PleI PpuMI PshAI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SphI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoI XhoII XmnI