################################## # NO CUT ENZYMES ( 80 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AgeI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I BamHI BciVI BclI BfrI BglI BsaBI BsaWI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI Bst1107I BstBI BstZ17I ClaI CpoI DraI EagI EclXI Eco47III Eco52I EcoRI FbaI FseI HpaI KpnI MamI MfeI MluI MroI MunI NaeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI ScaI SfiI SfuI SgrAI SnaBI SphI Sse8387I SspBI SspI SwaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 28 enzymes) # ################################### AccI 1408 GTAGAC GT^[AC][GT]AC AhdI 1012 GACAGTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 2353 GGGCCC GGGCC^C AspEI 1012 GACAGTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1156 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 552 TGCGCA TGC^GCA BbvCI 2961 GCTGAGG CCTCAGC(-5/-2) BcgI 1977 CGACGCCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 1087 AGATCT A^GATCT BsrBI 331 GAGCGG CCGCTC(-3/-3) DrdI 3397 GACTTGCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 1012 GACAGTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRV 1616 GATATC GAT^ATC EcoT22I 2475 ATGCAT ATGCA^T FspI 552 TGCGCA TGC^GCA KspI 50 CCGCGG CCGC^GG NdeI 2472 CATATG CA^TATG NsiI 2475 ATGCAT ATGCA^T PflFI 1156 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 1303 GACATTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacII 50 CCGCGG CCGC^GG SmaI 3034 CCCGGG CCC^GGG SpeI 862 ACTAGT A^CTAGT StuI 1692 AGGCCT AGG^CCT Tth111I 1156 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 2268 TCTAGA T^CTAGA XmaCI 3034 CCCGGG C^CCGGG XmaI 3034 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 37 enzymes) # ################################### AflIII 744 ACGTGT A^C[GA][CT]GT 1708 ACGTGT AocI 606 CCTTAGG CC^T[ACGT]AGG 1664 CCTCAGG AvaI 2104 CCCGAG C^[CT]CG[GA]G 3034 CCCGGG AvrII 1741 CCTAGG C^CTAGG 2133 CCTAGG BbrPI 90 CACGTG CAC^GTG 1707 CACGTG BbsI 3133 GAAGAC GAAGAC(2/6) 2869 GTCTTC BlnI 1741 CCTAGG C^CTAGG 2133 CCTAGG BlpI 328 GCTGAGC GC^T[ACGT]AGC 523 GCTGAGC Bpu1102I 328 GCTGAGC GC^T[ACGT]AGC 523 GCTGAGC BpuAI 3133 GAAGAC GAAGAC(2/6) 2869 GTCTTC BsaAI 90 CACGTG [CT]AC^GT[GA] 1707 CACGTG BsaI 673 GAGACC GGTCTC(1/5) 714 GAGACC BsoBI 2104 CCCGAG C^[CT]CG[GA]G 3034 CCCGGG BsrFI 125 ACCGGC [GA]^CCGG[CT] 2091 ACCGGC BssHII 40 GCGCGC G^CGCGC 95 GCGCGC BssSI 483 CACGAG CACGAG(-5/-1) 2228 CTCGTG BstEII 1962 GGTGACC G^GT[ACGT]ACC 2164 GGTAACC BstPI 1962 GGTGACC G^GT[ACGT]ACC 2164 GGTAACC BstXI 1022 CCACTCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1241 CCAACTTAGTGG Bsu36I 606 CCTTAGG CC^T[ACGT]AGG 1664 CCTCAGG CelII 328 GCTGAGC GC^T[ACGT]AGC 523 GCTGAGC Cfr10I 125 ACCGGC [GA]^CCGG[CT] 2091 ACCGGC DraIII 250 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG 3198 CACACTGTG Eco81I 606 CCTTAGG CC^T[ACGT]AGG 1664 CCTCAGG EcoO65I 1962 GGTGACC G^GT[ACGT]ACC 2164 GGTAACC HincII 174 GTTGAC GT[CT]^[GA]AC 2911 GTCAAC HindII 174 GTTGAC GT[CT]^[GA]AC 2911 GTCAAC MseI 2150 TTAA T^TAA 2810 TTAA MslI 2468 CACACATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2808 CATTAAGATG PmaCI 90 CACGTG CAC^GTG 1707 CACGTG PmlI 90 CACGTG CAC^GTG 1707 CACGTG SacI 2138 GAGCTC GAGCT^C 2322 GAGCTC SexAI 251 ACCAGGT A^CC[AT]GGT 3041 ACCTGGT TaqI 5 TCGA T^CGA 503 TCGA Tru9I 2150 TTAA T^TAA 2810 TTAA TthHB8I 5 TCGA T^CGA 503 TCGA XcmI 509 CCACTAGCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1505 CCAGACCCCTCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 113 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI AspHI AvaII BalI BanI BanII BbeI BbvI BcnI BfaI BmyI BpmI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspMI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HindIII HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MspA1I MspI MvaI MvnI MwoI NarI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI StyI TfiI TseI Tsp45I Tsp509I TspRI Van91I XhoII