##################################
# NO CUT ENZYMES (   80 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AgeI        Alw44I      Aor51HI     ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     BamHI       
BciVI       BclI        BfrI        BglI        BsaBI       
BsaWI       BseAI       BsiEI       BsiWI       BsmBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrGI       Bst1107I    BstBI       BstZ17I     ClaI        
CpoI        DraI        EagI        EclXI       Eco47III    
Eco52I      EcoRI       FbaI        FseI        HpaI        
KpnI        MamI        MfeI        MluI        MroI        
MunI        NaeI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PaeR7I      PinAI       
PmeI        PshBI       Psp1406I    PvuI        RcaI        
RsrII       SalI        SbfI        ScaI        SfiI        
SfuI        SgrAI       SnaBI       SphI        Sse8387I    
SspBI       SspI        SwaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   28 enzymes) #
###################################

AccI         1408  GTAGAC               GT^[AC][GT]AC
AhdI         1012  GACAGTATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI         2353  GGGCCC               GGGCC^C
AspEI        1012  GACAGTATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1156  GACTATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         552  TGCGCA               TGC^GCA
BbvCI        2961  GCTGAGG              CCTCAGC(-5/-2)
BcgI         1977  CGACGCCACTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII        1087  AGATCT               A^GATCT
BsrBI         331  GAGCGG               CCGCTC(-3/-3)
DrdI         3397  GACTTGCCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I     1012  GACAGTATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV        1616  GATATC               GAT^ATC
EcoT22I      2475  ATGCAT               ATGCA^T
FspI          552  TGCGCA               TGC^GCA
KspI           50  CCGCGG               CCGC^GG
NdeI         2472  CATATG               CA^TATG
NsiI         2475  ATGCAT               ATGCA^T
PflFI        1156  GACTATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI        1303  GACATTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII          50  CCGCGG               CCGC^GG
SmaI         3034  CCCGGG               CCC^GGG
SpeI          862  ACTAGT               A^CTAGT
StuI         1692  AGGCCT               AGG^CCT
Tth111I      1156  GACTATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         2268  TCTAGA               T^CTAGA
XmaCI        3034  CCCGGG               C^CCGGG
XmaI         3034  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   37 enzymes) #
###################################

AflIII        744  ACGTGT               A^C[GA][CT]GT
             1708  ACGTGT              
AocI          606  CCTTAGG              CC^T[ACGT]AGG
             1664  CCTCAGG             
AvaI         2104  CCCGAG               C^[CT]CG[GA]G
             3034  CCCGGG              
AvrII        1741  CCTAGG               C^CTAGG
             2133  CCTAGG              
BbrPI          90  CACGTG               CAC^GTG
             1707  CACGTG              
BbsI         3133  GAAGAC               GAAGAC(2/6)
             2869  GTCTTC              
BlnI         1741  CCTAGG               C^CTAGG
             2133  CCTAGG              
BlpI          328  GCTGAGC              GC^T[ACGT]AGC
              523  GCTGAGC             
Bpu1102I      328  GCTGAGC              GC^T[ACGT]AGC
              523  GCTGAGC             
BpuAI        3133  GAAGAC               GAAGAC(2/6)
             2869  GTCTTC              
BsaAI          90  CACGTG               [CT]AC^GT[GA]
             1707  CACGTG              
BsaI          673  GAGACC               GGTCTC(1/5)
              714  GAGACC              
BsoBI        2104  CCCGAG               C^[CT]CG[GA]G
             3034  CCCGGG              
BsrFI         125  ACCGGC               [GA]^CCGG[CT]
             2091  ACCGGC              
BssHII         40  GCGCGC               G^CGCGC
               95  GCGCGC              
BssSI         483  CACGAG               CACGAG(-5/-1)
             2228  CTCGTG              
BstEII       1962  GGTGACC              G^GT[ACGT]ACC
             2164  GGTAACC             
BstPI        1962  GGTGACC              G^GT[ACGT]ACC
             2164  GGTAACC             
BstXI        1022  CCACTCCCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1241  CCAACTTAGTGG        
Bsu36I        606  CCTTAGG              CC^T[ACGT]AGG
             1664  CCTCAGG             
CelII         328  GCTGAGC              GC^T[ACGT]AGC
              523  GCTGAGC             
Cfr10I        125  ACCGGC               [GA]^CCGG[CT]
             2091  ACCGGC              
DraIII        250  CACCAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             3198  CACACTGTG           
Eco81I        606  CCTTAGG              CC^T[ACGT]AGG
             1664  CCTCAGG             
EcoO65I      1962  GGTGACC              G^GT[ACGT]ACC
             2164  GGTAACC             
HincII        174  GTTGAC               GT[CT]^[GA]AC
             2911  GTCAAC              
HindII        174  GTTGAC               GT[CT]^[GA]AC
             2911  GTCAAC              
MseI         2150  TTAA                 T^TAA
             2810  TTAA                
MslI         2468  CACACATATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2808  CATTAAGATG          
PmaCI          90  CACGTG               CAC^GTG
             1707  CACGTG              
PmlI           90  CACGTG               CAC^GTG
             1707  CACGTG              
SacI         2138  GAGCTC               GAGCT^C
             2322  GAGCTC              
SexAI         251  ACCAGGT              A^CC[AT]GGT
             3041  ACCTGGT             
TaqI            5  TCGA                 T^CGA
              503  TCGA                
Tru9I        2150  TTAA                 T^TAA
             2810  TTAA                
TthHB8I         5  TCGA                 T^CGA
              503  TCGA                
XcmI          509  CCACTAGCCTGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1505  CCAGACCCCTCCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  113 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AluI        AlwI        AlwNI       ApoI        AspHI       
AvaII       BalI        BanI        BanII       BbeI        
BbvI        BcnI        BfaI        BmyI        BpmI        
BsaHI       BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsmI        
Bsp1286I    BspMI       BsrDI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstUI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HindIII     HinfI       
HpaII       HphI        ItaI        KasI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MspA1I      
MspI        MvaI        MvnI        MwoI        NarI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
NspI        PflMI       PleI        PpuMI       PstI        
PvuII       RsaI        SapI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SfoI        SmlI        
StyI        TfiI        TseI        Tsp45I      Tsp509I     
TspRI       Van91I      XhoII