################################## # NO CUT ENZYMES ( 107 enzymes) # ################################## AatII AccIII AclI AflII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I AspEI AspI AviII BamHI BbrPI BcgI BciVI BclI BfrI BglII BlpI Bpu1102I BsaAI BsaBI BseAI BsiEI BsmI BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BssHII BstAPI BstBI CelII Cfr10I ClaI CpoI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KspI MamI MfeI MroI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI TaqI Tth111I TthHB8I Van91I XhoI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccI 872 GTATAC GT^[AC][GT]AC AcsI 317 GAATTC [GA]^AATT[CT] AcyI 245 GGCGCC G[GA]^CG[CT]C AflIII 1194 ACGCGT A^C[GA][CT]GT ApaI 501 GGGCCC GGGCC^C ApoI 317 GAATTC [GA]^AATT[CT] AvrII 512 CCTAGG C^CTAGG BalI 200 TGGCCA TGG^CCA BbeI 245 GGCGCC GGCGC^C BbsI 115 GTCTTC GAAGAC(2/6) BlnI 512 CCTAGG C^CTAGG BpuAI 115 GTCTTC GAAGAC(2/6) BsaHI 245 GGCGCC G[GA]^CG[CT]C BsaWI 1175 TCCGGT [AT]^CCGG[AT] BsiWI 1197 CGTACG C^GTACG BsmBI 1284 CGTCTC CGTCTC(1/5) BssSI 1147 CTCGTG CACGAG(-5/-1) Bst1107I 872 GTATAC GTA^TAC BstEII 1811 GGTTACC G^GT[ACGT]ACC BstPI 1811 GGTTACC G^GT[ACGT]ACC BstYI 1142 AGATCC [GA]^GATC[CT] BstZ17I 872 GTATAC GTA^TAC CfoI 246 GCGC GCG^C DraI 2651 TTTAAA TTT^AAA DrdI 1313 GACTTCTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 267 CCGTGG C^C[GA][CT]GG EaeI 200 TGGCCA [CT]^GGCC[GA] EcoO65I 1811 GGTTACC G^GT[ACGT]ACC EcoRI 317 GAATTC G^AATTC HaeII 245 GGCGCC [GA]GCGC^[CT] HhaI 246 GCGC GCG^C Hin1I 245 GGCGCC G[GA]^CG[CT]C HinP1I 246 GCGC G^CGC KasI 245 GGCGCC G^GCGCC MaeII 1059 ACGT A^CGT MflI 1142 AGATCC [GA]^GATC[CT] MluI 1194 ACGCGT A^CGCGT MluNI 200 TGGCCA TGG^CCA MscI 200 TGGCCA TGG^CCA MslI 1096 CATTACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 245 GGCGCC GG^CGCC NspI 2561 GCATGT [GA]CATG^[CT] PstI 1700 CTGCAG CTGCA^G SapI 1848 GCTCTTC GCTCTTC(1/4) SexAI 1796 ACCTGGT A^CC[AT]GGT SfoI 245 GGCGCC GGC^GCC StuI 849 AGGCCT AGG^CCT Tsp509I 318 AATT ^AATT XbaI 1839 TCTAGA T^CTAGA XhoII 1142 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### Acc65I 281 GGTACC G^GTACC 521 GGTACC AccII 289 CGCG CG^CG 1195 CGCG AocI 1266 CCTCAGG CC^T[ACGT]AGG 1343 CCTCAGG Asp718I 281 GGTACC G^GTACC 521 GGTACC BglI 354 GCCGCAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 738 GCCGTACAGGC BsaI 1625 GGTCTC GGTCTC(1/5) 2617 GGTCTC BsgI 221 CTGCAC GTGCAG(16/14) 1817 CTGCAC BspMI 576 ACCTGC ACCTGC(4/8) 1815 ACCTGC BstUI 289 CGCG CG^CG 1195 CGCG Bsu36I 1266 CCTCAGG CC^T[ACGT]AGG 1343 CCTCAGG Eco81I 1266 CCTCAGG CC^T[ACGT]AGG 1343 CCTCAGG HgaI 704 GACGC GACGC(5/10) 1283 GCGTC KpnI 281 GGTACC GGTAC^C 521 GGTACC MseI 666 TTAA T^TAA 2652 TTAA MvnI 289 CGCG CG^CG 1195 CGCG PvuII 806 CAGCTG CAG^CTG 1185 CAGCTG SfcI 1700 CTGCAG C^T[GA][CT]AG 1973 CTGTAG SmaI 504 CCCGGG CCC^GGG 893 CCCGGG SmlI 683 CTCAAG C^T[CT][GA]AG 1437 CTCAAG Tru9I 666 TTAA T^TAA 2652 TTAA XmaCI 504 CCCGGG C^CCGGG 893 CCCGGG XmaI 504 CCCGGG C^CCGGG 893 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 79 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BanI BanII BbvCI BbvI BcnI BfaI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BsrGI BsrI BssKI BstF5I BstNI BstXI Cac8I Cfr13I DdeI DpnII DraII EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI SspBI StyI TfiI TseI Tsp45I TspRI XcmI