##################################
# NO CUT ENZYMES (   75 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AgeI        AhdI        ApaI        ApoI        
AscI        Asp718I     AspEI       AviII       AvrII       
BamHI       BbrPI       BbvCI       BfrI        BlnI        
BsaAI       BsaBI       BseAI       BsiWI       BsmBI       
BsmI        BspDI       BspEI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstZ17I     
ClaI        DraI        Eam1105I    EcoO65I     EcoRI       
EcoRV       EcoT22I     FseI        FspI        HpaI        
KpnI        KspI        MamI        MfeI        MluI        
MroI        MunI        NdeI        NotI        NruI        
NsiI        NspV        PacI        PinAI       PmaCI       
PmeI        PmlI        Psp1406I    PvuI        SacII       
SalI        SapI        SbfI        SfiI        SfuI        
SgrAI       SnaBI       SpeI        Sse8387I    SwaI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AccI         3087  GTCTAC               GT^[AC][GT]AC
Aor51HI       136  AGCGCT               AGC^GCT
AseI          386  ATTAAT               AT^TAAT
AsnI          386  ATTAAT               AT^TAAT
Asp700I       193  GAACAGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BciVI         408  GTATCC               GTATCC(6/5)
BclI         1201  TGATCA               T^GATCA
BglII        2772  AGATCT               A^GATCT
BsiEI           5  CGGCCG               CG[GA][CT]^CG
BspHI         528  TCATGA               T^CATGA
BsrFI         176  GCCGGC               [GA]^CCGG[CT]
Cfr10I        176  GCCGGC               [GA]^CCGG[CT]
CpoI         1694  CGGACCG              CG^G[AT]CCG
DrdI         3486  GACATAGTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI            5  CGGCCG               C^GGCCG
EclXI           5  CGGCCG               C^GGCCG
Eco47III      136  AGCGCT               AGC^GCT
Eco52I          5  CGGCCG               C^GGCCG
FbaI         1201  TGATCA               T^GATCA
HincII       1456  GTTGAC               GT[CT]^[GA]AC
HindII       1456  GTTGAC               GT[CT]^[GA]AC
HindIII      1987  AAGCTT               A^AGCTT
NaeI          176  GCCGGC               GCC^GGC
NgoMIV        176  GCCGGC               G^CCGGC
PaeR7I       1323  CTCGAG               C^TCGAG
PshAI        3120  GACACCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI         386  ATTAAT               AT^TAAT
RcaI          528  TCATGA               T^CATGA
RsrII        1694  CGGACCG              CG^G[AT]CCG
ScaI          747  AGTACT               AGT^ACT
SmaI          127  CCCGGG               CCC^GGG
SphI         3512  GCATGC               GCATG^C
SspI         3902  AATATT               AAT^ATT
XbaI         2940  TCTAGA               T^CTAGA
XhoI         1323  CTCGAG               C^TCGAG
XmaCI         127  CCCGGG               C^CCGGG
XmaI          127  CCCGGG               C^CCGGG
XmnI          193  GAACAGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AcyI           52  GGCGCC               G[GA]^CG[CT]C
             2803  GGCGCC              
Alw44I        331  GTGCAC               G^TGCAC
             2601  GTGCAC              
ApaLI         331  GTGCAC               G^TGCAC
             2601  GTGCAC              
AspI         1411  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2617  GACTGTGTC           
BbeI           52  GGCGCC               GGCGC^C
             2803  GGCGCC              
BglI          176  GCCGGCGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1590  GCCTCATGGGC         
BsaHI          52  GGCGCC               G[GA]^CG[CT]C
             2803  GGCGCC              
Bsp1407I      213  TGTACA               T^GTACA
             2846  TGTACA              
BspLU11I      942  ACATGT               A^CATGT
             2859  ACATGT              
BsrBI         355  CCGCTC               CCGCTC(-3/-3)
             1034  GAGCGG              
BsrDI        3533  CATTGC               GCAATG(2/0)
             3750  GCAATG              
BsrGI         213  TGTACA               T^GTACA
             2846  TGTACA              
DraIII       1092  CACCCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1272  CACCCAGTG           
EarI         1125  CTCTTC               CTCTTC(1/4)
             3307  CTCTTC              
HgaI         1459  GACGC                GACGC(5/10)
              441  GCGTC               
Hin1I          52  GGCGCC               G[GA]^CG[CT]C
             2803  GGCGCC              
KasI           52  GGCGCC               G^GCGCC
             2803  GGCGCC              
Ksp632I      1125  CTCTTC               CTCTTC(1/4)
             3307  CTCTTC              
NarI           52  GGCGCC               GG^CGCC
             2803  GGCGCC              
NheI         1316  GCTAGC               G^CTAGC
             2477  GCTAGC              
PflFI        1411  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2617  GACTGTGTC           
PflMI        1784  CCAGGCCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3538  CCACTTGCTGG         
SacI         1064  GAGCTC               GAGCT^C
             3586  GAGCTC              
SfoI           52  GGCGCC               GGC^GCC
             2803  GGCGCC              
SmlI           76  CTCAAG               C^T[CT][GA]AG
             1323  CTCGAG              
SspBI         213  TGTACA               T^GTACA
             2846  TGTACA              
Tth111I      1411  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2617  GACTGTGTC           
Van91I       1784  CCAGGCCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3538  CCACTTGCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (  117 enzymes) #
#########################################

AccII       AciI        AfaI        AflIII      AluI        
AlwI        AlwNI       AocI        AspHI       AvaI        
AvaII       BalI        BanI        BanII       BbsI        
BbvI        BcgI        BcnI        BfaI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaI        
BsaJI       BsaWI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrI        BssKI       BstAPI      
BstF5I      BstNI       BstUI       BstXI       BstYI       
Bsu36I      Cac8I       CelII       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
Eco81I      EcoNI       EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NcoI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PpuMI       PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SexAI       SfaNI       SfcI        
StuI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XcmI        XhoII