################################## # NO CUT ENZYMES ( 75 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AgeI AhdI ApaI ApoI AscI Asp718I AspEI AviII AvrII BamHI BbrPI BbvCI BfrI BlnI BsaAI BsaBI BseAI BsiWI BsmBI BsmI BspDI BspEI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I ClaI DraI Eam1105I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HpaI KpnI KspI MamI MfeI MluI MroI MunI NdeI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI Psp1406I PvuI SacII SalI SapI SbfI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SwaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AccI 3087 GTCTAC GT^[AC][GT]AC Aor51HI 136 AGCGCT AGC^GCT AseI 386 ATTAAT AT^TAAT AsnI 386 ATTAAT AT^TAAT Asp700I 193 GAACAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BciVI 408 GTATCC GTATCC(6/5) BclI 1201 TGATCA T^GATCA BglII 2772 AGATCT A^GATCT BsiEI 5 CGGCCG CG[GA][CT]^CG BspHI 528 TCATGA T^CATGA BsrFI 176 GCCGGC [GA]^CCGG[CT] Cfr10I 176 GCCGGC [GA]^CCGG[CT] CpoI 1694 CGGACCG CG^G[AT]CCG DrdI 3486 GACATAGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 5 CGGCCG C^GGCCG EclXI 5 CGGCCG C^GGCCG Eco47III 136 AGCGCT AGC^GCT Eco52I 5 CGGCCG C^GGCCG FbaI 1201 TGATCA T^GATCA HincII 1456 GTTGAC GT[CT]^[GA]AC HindII 1456 GTTGAC GT[CT]^[GA]AC HindIII 1987 AAGCTT A^AGCTT NaeI 176 GCCGGC GCC^GGC NgoMIV 176 GCCGGC G^CCGGC PaeR7I 1323 CTCGAG C^TCGAG PshAI 3120 GACACCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 386 ATTAAT AT^TAAT RcaI 528 TCATGA T^CATGA RsrII 1694 CGGACCG CG^G[AT]CCG ScaI 747 AGTACT AGT^ACT SmaI 127 CCCGGG CCC^GGG SphI 3512 GCATGC GCATG^C SspI 3902 AATATT AAT^ATT XbaI 2940 TCTAGA T^CTAGA XhoI 1323 CTCGAG C^TCGAG XmaCI 127 CCCGGG C^CCGGG XmaI 127 CCCGGG C^CCGGG XmnI 193 GAACAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AcyI 52 GGCGCC G[GA]^CG[CT]C 2803 GGCGCC Alw44I 331 GTGCAC G^TGCAC 2601 GTGCAC ApaLI 331 GTGCAC G^TGCAC 2601 GTGCAC AspI 1411 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2617 GACTGTGTC BbeI 52 GGCGCC GGCGC^C 2803 GGCGCC BglI 176 GCCGGCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1590 GCCTCATGGGC BsaHI 52 GGCGCC G[GA]^CG[CT]C 2803 GGCGCC Bsp1407I 213 TGTACA T^GTACA 2846 TGTACA BspLU11I 942 ACATGT A^CATGT 2859 ACATGT BsrBI 355 CCGCTC CCGCTC(-3/-3) 1034 GAGCGG BsrDI 3533 CATTGC GCAATG(2/0) 3750 GCAATG BsrGI 213 TGTACA T^GTACA 2846 TGTACA DraIII 1092 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG 1272 CACCCAGTG EarI 1125 CTCTTC CTCTTC(1/4) 3307 CTCTTC HgaI 1459 GACGC GACGC(5/10) 441 GCGTC Hin1I 52 GGCGCC G[GA]^CG[CT]C 2803 GGCGCC KasI 52 GGCGCC G^GCGCC 2803 GGCGCC Ksp632I 1125 CTCTTC CTCTTC(1/4) 3307 CTCTTC NarI 52 GGCGCC GG^CGCC 2803 GGCGCC NheI 1316 GCTAGC G^CTAGC 2477 GCTAGC PflFI 1411 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2617 GACTGTGTC PflMI 1784 CCAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3538 CCACTTGCTGG SacI 1064 GAGCTC GAGCT^C 3586 GAGCTC SfoI 52 GGCGCC GGC^GCC 2803 GGCGCC SmlI 76 CTCAAG C^T[CT][GA]AG 1323 CTCGAG SspBI 213 TGTACA T^GTACA 2846 TGTACA Tth111I 1411 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2617 GACTGTGTC Van91I 1784 CCAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3538 CCACTTGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 117 enzymes) # ######################################### AccII AciI AfaI AflIII AluI AlwI AlwNI AocI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcgI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII