################################## # NO CUT ENZYMES ( 80 enzymes) # ################################## Acc65I AccIII AclI AflIII AgeI AhdI Aor51HI AscI Asp718I AspEI AviII BamHI BanI BbeI BbrPI BcgI BglII BsaAI BsaBI BsaWI BseAI BsiWI BsmBI BspDI BspEI BspLU11I BsrBI BssHII BstAPI BstBI ClaI CpoI DraIII DrdI Eam1105I Eco47III EcoRI EcoT22I FseI FspI HpaI KasI KpnI MamI MfeI MluI MroI MunI NarI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI PvuII RsrII SacI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SphI Sse8387I StuI SwaI XhoI ################################### # ONE CUT ENZYMES ( 61 enzymes) # ################################### AatII 46 GACGTC GACGT^C AcyI 46 GACGTC G[GA]^CG[CT]C Alw44I 1816 GTGCAC G^TGCAC AlwI 348 GATCC GGATC(4/5) AocI 1956 CCTTAGG CC^T[ACGT]AGG ApaI 328 GGGCCC GGGCC^C ApaLI 1816 GTGCAC G^TGCAC Asp700I 2463 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 1816 GTGCAC G[AT]GC[AT]^C AvaI 1315 CCCGGG C^[CT]CG[GA]G AvrII 2365 CCTAGG C^CTAGG BalI 1122 TGGCCA TGG^CCA BbvCI 3250 GCTGAGG CCTCAGC(-5/-2) BclI 1661 TGATCA T^GATCA BglI 3262 GCCTGCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 2365 CCTAGG C^CTAGG BsaHI 46 GACGTC G[GA]^CG[CT]C BsaI 1446 GGTCTC GGTCTC(1/5) BsiEI 1159 CGGCCG CG[GA][CT]^CG BsiHKAI 1816 GTGCAC G[AT]GC[AT]^C BsoBI 1315 CCCGGG C^[CT]CG[GA]G Bsp1407I 2299 TGTACA T^GTACA BspHI 2712 TCATGA T^CATGA BsrDI 123 CATTGC GCAATG(2/0) BsrFI 1 GCCGGC [GA]^CCGG[CT] BsrGI 2299 TGTACA T^GTACA BssSI 175 CACGAG CACGAG(-5/-1) BstEII 2241 GGTGACC G^GT[ACGT]ACC BstPI 2241 GGTGACC G^GT[ACGT]ACC BstYI 347 AGATCC [GA]^GATC[CT] Bsu36I 1956 CCTTAGG CC^T[ACGT]AGG Cfr10I 1 GCCGGC [GA]^CCGG[CT] EagI 1159 CGGCCG C^GGCCG EclXI 1159 CGGCCG C^GGCCG Eco52I 1159 CGGCCG C^GGCCG Eco81I 1956 CCTTAGG CC^T[ACGT]AGG EcoNI 403 CCTGACTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 2241 GGTGACC G^GT[ACGT]ACC EcoRV 92 GATATC GAT^ATC FbaI 1661 TGATCA T^GATCA Hin1I 46 GACGTC G[GA]^CG[CT]C HincII 295 GTCGAC GT[CT]^[GA]AC HindII 295 GTCGAC GT[CT]^[GA]AC KspI 1162 CCGCGG CCGC^GG MflI 347 AGATCC [GA]^GATC[CT] MluNI 1122 TGGCCA TGG^CCA MscI 1122 TGGCCA TGG^CCA MspA1I 1162 CCGCGG C[AC]G^C[GT]G NaeI 1 GCCGGC GCC^GGC NdeI 3341 CATATG CA^TATG NgoMIV 1 GCCGGC G^CCGGC RcaI 2712 TCATGA T^CATGA SacII 1162 CCGCGG CCGC^GG SalI 295 GTCGAC G^TCGAC SmaI 1315 CCCGGG CCC^GGG SspBI 2299 TGTACA T^GTACA XbaI 1886 TCTAGA T^CTAGA XhoII 347 AGATCC [GA]^GATC[CT] XmaCI 1315 CCCGGG C^CCGGG XmaI 1315 CCCGGG C^CCGGG XmnI 2463 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccII 360 CGCG CG^CG 1163 CGCG AflII 589 CTTAAG C^TTAAG 2971 CTTAAG AseI 2336 ATTAAT AT^TAAT 2723 ATTAAT AsnI 2336 ATTAAT AT^TAAT 2723 ATTAAT AspI 2705 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 3350 GACCCTGTC BfrI 589 CTTAAG C^TTAAG 2971 CTTAAG BsmI 1405 GAATGC GAATGC(1/-1) 577 GCATTC BspMI 544 ACCTGC ACCTGC(4/8) 3411 ACCTGC Bst1107I 211 GTATAC GTA^TAC 519 GTATAC BstUI 360 CGCG CG^CG 1163 CGCG BstXI 1191 CCACAACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2278 CCAGAGTTCTGG BstZ17I 211 GTATAC GTA^TAC 519 GTATAC CfoI 180 GCGC GCG^C 1265 GCGC FauI 949 CCCGC CCCGC(4/6) 1164 GCGGG HaeII 179 AGCGCC [GA]GCGC^[CT] 1264 GGCGCT HgaI 358 GACGC GACGC(5/10) 1222 GACGC HhaI 180 GCGC GCG^C 1265 GCGC HinP1I 180 GCGC G^CGC 1265 GCGC HindIII 2560 AAGCTT A^AGCTT 2832 AAGCTT MaeII 47 ACGT A^CGT 1802 ACGT MvnI 360 CGCG CG^CG 1163 CGCG NcoI 1758 CCATGG C^CATGG 3257 CCATGG NspI 994 ACATGC [GA]CATG^[CT] 3439 GCATGT PflFI 2705 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 3350 GACCCTGTC PflMI 1097 CCACTTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1578 CCATTGTTTGG PshBI 2336 ATTAAT AT^TAAT 2723 ATTAAT PstI 486 CTGCAG CTGCA^G 546 CTGCAG SpeI 1671 ACTAGT A^CTAGT 2348 ACTAGT SspI 2902 AATATT AAT^ATT 3463 AATATT Tth111I 2705 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 3350 GACCCTGTC Van91I 1097 CCACTTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1578 CCATTGTTTGG XcmI 1426 CCAACAGCCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3257 CCATGGCCTGCGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 85 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwNI ApoI AvaII BanII BbsI BbvI BciVI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CelII Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I