##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AclI AflII AgeI AhdI AscI
AseI AsnI AspEI AspI BamHI
BbeI BbrPI BciVI BfrI BsaAI
BsaBI BsiWI BspDI BspHI BsrFI
Bst1107I BstAPI BstZ17I Cfr10I ClaI
DraI DrdI EagI Eam1105I EclXI
Eco52I EcoRI EcoRV EcoT22I FseI
HindIII HpaI KasI MamI MfeI
MluI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI SalI SbfI ScaI SfiI
SfoI SgrAI SnaBI SpeI SphI
Sse8387I StuI SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 2778 GACGTC GACGT^C
Acc65I 2848 GGTACC G^GTACC
AccI 2338 GTCTAC GT^[AC][GT]AC
AcsI 1462 GAATTT [GA]^AATT[CT]
AflIII 2466 ACATGT A^C[GA][CT]GT
AocI 1996 CCTGAGG CC^T[ACGT]AGG
ApaI 1407 GGGCCC GGGCC^C
ApoI 1462 GAATTT [GA]^AATT[CT]
Asp718I 2848 GGTACC G^GTACC
AvaI 1410 CCCGGG C^[CT]CG[GA]G
AvrII 1670 CCTAGG C^CTAGG
BbvCI 211 GCTGAGG CCTCAGC(-5/-2)
BcgI 1828 CGAAGTCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 1670 CCTAGG C^CTAGG
BlpI 2062 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2062 GCTCAGC GC^T[ACGT]AGC
BsiEI 2038 CGACCG CG[GA][CT]^CG
BsmI 3169 GCATTC GAATGC(1/-1)
BsoBI 1410 CCCGGG C^[CT]CG[GA]G
BspLU11I 2466 ACATGT A^CATGT
BsrBI 156 GAGCGG CCGCTC(-3/-3)
BssHII 140 GCGCGC G^CGCGC
BssSI 3349 CTCGTG CACGAG(-5/-1)
BstBI 1826 TTCGAA TT^CGAA
BstEII 2485 GGTGACC G^GT[ACGT]ACC
BstPI 2485 GGTGACC G^GT[ACGT]ACC
Bsu36I 1996 CCTGAGG CC^T[ACGT]AGG
CelII 2062 GCTCAGC GC^T[ACGT]AGC
CpoI 613 CGGACCG CG^G[AT]CCG
Eco81I 1996 CCTGAGG CC^T[ACGT]AGG
EcoO65I 2485 GGTGACC G^GT[ACGT]ACC
HincII 690 GTCAAC GT[CT]^[GA]AC
HindII 690 GTCAAC GT[CT]^[GA]AC
KpnI 2848 GGTACC GGTAC^C
KspI 68 CCGCGG CCGC^GG
MseI 3561 TTAA T^TAA
NcoI 2526 CCATGG C^CATGG
NspV 1826 TTCGAA TT^CGAA
PshAI 1699 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 2377 CTGCAG CTGCA^G
RsrII 613 CGGACCG CG^G[AT]CCG
SacII 68 CCGCGG CCGC^GG
SapI 2169 GCTCTTC GCTCTTC(1/4)
SfuI 1826 TTCGAA TT^CGAA
SmaI 1410 CCCGGG CCC^GGG
Tru9I 3561 TTAA T^TAA
Tsp509I 1463 AATT ^AATT
XbaI 572 TCTAGA T^CTAGA
XmaCI 1410 CCCGGG C^CCGGG
XmaI 1410 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Alw44I 484 GTGCAC G^TGCAC
2156 GTGCAC
ApaLI 484 GTGCAC G^TGCAC
2156 GTGCAC
Asp700I 998 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1819 GAAGGAGTTC
AviII 294 TGCGCA TGC^GCA
390 TGCGCA
BclI 264 TGATCA T^GATCA
1797 TGATCA
BglI 557 GCCCTCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3033 GCCAACTGGGC
BglII 757 AGATCT A^GATCT
1581 AGATCT
BstXI 900 CCAGCCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
966 CCAGCCCAGTGG
BstYI 757 AGATCT [GA]^GATC[CT]
1581 AGATCT
DraIII 431 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
3432 CACGCAGTG
FbaI 264 TGATCA T^GATCA
1797 TGATCA
FspI 294 TGCGCA TGC^GCA
390 TGCGCA
MflI 757 AGATCT [GA]^GATC[CT]
1581 AGATCT
NspI 744 ACATGC [GA]CATG^[CT]
2466 ACATGT
PflMI 1255 CCACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2410 CCAGCAGGTGG
SexAI 432 ACCTGGT A^CC[AT]GGT
1749 ACCTGGT
SspI 3515 AATATT AAT^ATT
3552 AATATT
Van91I 1255 CCACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2410 CCAGCAGGTGG
XcmI 966 CCAGCCCAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2175 CCAGCTCTGATGTGG
XhoII 757 AGATCT [GA]^GATC[CT]
1581 AGATCT
XmnI 998 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1819 GAAGGAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccII AccIII AciI AcyI AfaI
AluI AlwI AlwNI Aor51HI AspHI
AvaII BalI BanI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BsaWI
BseAI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI Bsp1286I
Bsp1407I BspEI BspMI BsrDI BsrGI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco47III
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MroI MscI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
StyI TaqI TfiI TseI Tsp45I
TspRI TthHB8I