################################## # NO CUT ENZYMES ( 50 enzymes) # ################################## AccIII AclI AgeI AscI AseI AsnI BamHI BbrPI BcgI BciVI BseAI BsiEI BsiWI BspDI BspEI BssHII Bst1107I BstBI BstZ17I ClaI CpoI EagI EclXI Eco52I EcoRV FseI HgaI HindIII MluI MroI NdeI NotI NruI NspV PacI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SalI SfiI SfuI SgrAI SnaBI SpeI SwaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AatII 3056 GACGTC GACGT^C Acc65I 4324 GGTACC G^GTACC AflII 282 CTTAAG C^TTAAG AhdI 1402 GACCTTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 737 GTGCAC G^TGCAC Aor51HI 669 AGCGCT AGC^GCT ApaLI 737 GTGCAC G^TGCAC Asp718I 4324 GGTACC G^GTACC AspEI 1402 GACCTTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 4099 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 3508 TGCGCA TGC^GCA BbvCI 4186 GCTGAGG CCTCAGC(-5/-2) BclI 3856 TGATCA T^GATCA BfrI 282 CTTAAG C^TTAAG BglI 315 GCCGTGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1942 AGATCT A^GATCT BlpI 4117 GCTCAGC GC^T[ACGT]AGC Bpu1102I 4117 GCTCAGC GC^T[ACGT]AGC BsaAI 4879 CACGTA [CT]AC^GT[GA] BsaBI 2406 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmBI 3058 CGTCTC CGTCTC(1/5) Bsp1407I 2154 TGTACA T^GTACA BsrBI 4238 GAGCGG CCGCTC(-3/-3) BsrGI 2154 TGTACA T^GTACA BssSI 4136 CTCGTG CACGAG(-5/-1) CelII 4117 GCTCAGC GC^T[ACGT]AGC DraIII 1812 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 1402 GACCTTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 669 AGCGCT AGC^GCT EcoNI 3464 CCTGGGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 2880 ATGCAT ATGCA^T FbaI 3856 TGATCA T^GATCA FspI 3508 TGCGCA TGC^GCA HpaI 1301 GTTAAC GTT^AAC KpnI 4324 GGTACC GGTAC^C MamI 2406 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 5394 CAATTG C^AATTG MunI 5394 CAATTG C^AATTG NsiI 2880 ATGCAT ATGCA^T PaeR7I 4806 CTCGAG C^TCGAG PflFI 4099 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC SacI 3477 GAGCTC GAGCT^C SbfI 623 CCTGCAGG CCTGCA^GG SexAI 2293 ACCAGGT A^CC[AT]GGT Sse8387I 623 CCTGCAGG CCTGCA^GG SspBI 2154 TGTACA T^GTACA Tth111I 4099 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 4806 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccI 1383 GTCTAC GT^[AC][GT]AC 1796 GTCTAC AvrII 3962 CCTAGG C^CTAGG 4906 CCTAGG BbeI 84 GGCGCC GGCGC^C 4245 GGCGCC BbsI 3611 GAAGAC GAAGAC(2/6) 4277 GAAGAC BlnI 3962 CCTAGG C^CTAGG 4906 CCTAGG BpuAI 3611 GAAGAC GAAGAC(2/6) 4277 GAAGAC BsaWI 3872 ACCGGA [AT]^CCGG[AT] 4596 TCCGGT BsmI 3197 GAATGC GAATGC(1/-1) 4937 GCATTC BspHI 1903 TCATGA T^CATGA 2920 TCATGA BspLU11I 5143 ACATGT A^CATGT 6054 ACATGT DrdI 1787 GACCACAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 6042 GACATCGTGGTC EcoRI 748 GAATTC G^AATTC 5266 GAATTC KasI 84 GGCGCC G^GCGCC 4245 GGCGCC KspI 36 CCGCGG CCGC^GG 174 CCGCGG NaeI 16 GCCGGC GCC^GGC 4024 GCCGGC NarI 84 GGCGCC GG^CGCC 4245 GGCGCC NgoMIV 16 GCCGGC G^CCGGC 4024 GCCGGC PshAI 2532 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 4947 GACAGCTGTC RcaI 1903 TCATGA T^CATGA 2920 TCATGA SacII 36 CCGCGG CCGC^GG 174 CCGCGG SapI 3474 GAAGAGC GCTCTTC(1/4) 1498 GCTCTTC ScaI 1180 AGTACT AGT^ACT 1194 AGTACT SfoI 84 GGCGCC GGC^GCC 4245 GGCGCC SmaI 3282 CCCGGG CCC^GGG 5887 CCCGGG SphI 2622 GCATGC GCATG^C 3971 GCATGC SspI 2968 AATATT AAT^ATT 4408 AATATT XbaI 1929 TCTAGA T^CTAGA 1939 TCTAGA XmaCI 3282 CCCGGG C^CCGGG 5887 CCCGGG XmaI 3282 CCCGGG C^CCGGG 5887 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 131 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI AocI ApaI ApoI Asp700I AspHI AvaI AvaII BalI BanI BanII BbvI BcnI BfaI BmyI BpmI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BstAPI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI Eco81I EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NheI NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmnI