################################## # NO CUT ENZYMES ( 62 enzymes) # ################################## AclI AgeI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI AvaI AviII AvrII BbeI BcgI BciVI BglII BlnI BsiWI BsoBI BssHII Bst1107I BstAPI BstZ17I Bsu36I CpoI DrdI Eco47III Eco81I FseI FspI KasI KspI MfeI MluI MunI NarI NheI NotI NruI PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SexAI SfoI SgrAI SmaI SnaBI Sse8387I StuI SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AatII 3148 GACGTC GACGT^C AccI 3317 GTCTAC GT^[AC][GT]AC AccIII 934 TCCGGA T^CCGGA AflII 1806 CTTAAG C^TTAAG AflIII 1180 ACATGT A^C[GA][CT]GT AhdI 4969 GACGAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 2 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG AspEI 4969 GACGAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 782 GGATCC G^GATCC BbrPI 1074 CACGTG CAC^GTG BbvCI 1833 GCTGAGG CCTCAGC(-5/-2) BclI 2263 TGATCA T^GATCA BfrI 1806 CTTAAG C^TTAAG BglI 417 GCCTTTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 2431 GCTTAGC GC^T[ACGT]AGC Bpu1102I 2431 GCTTAGC GC^T[ACGT]AGC BsaWI 934 TCCGGA [AT]^CCGG[AT] BseAI 934 TCCGGA T^CCGGA BsiEI 349 CGGCCG CG[GA][CT]^CG BspDI 1290 ATCGAT AT^CGAT BspEI 934 TCCGGA T^CCGGA BspLU11I 1180 ACATGT A^CATGT BstYI 782 GGATCC [GA]^GATC[CT] CelII 2431 GCTTAGC GC^T[ACGT]AGC ClaI 1290 ATCGAT AT^CGAT EagI 349 CGGCCG C^GGCCG Eam1105I 4969 GACGAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 349 CGGCCG C^GGCCG Eco52I 349 CGGCCG C^GGCCG EcoRI 1869 GAATTC G^AATTC EcoRV 4401 GATATC GAT^ATC EcoT22I 3786 ATGCAT ATGCA^T FbaI 2263 TGATCA T^GATCA MflI 782 GGATCC [GA]^GATC[CT] MroI 934 TCCGGA T^CCGGA NaeI 3088 GCCGGC GCC^GGC NdeI 5459 CATATG CA^TATG NgoMIV 3088 GCCGGC G^CCGGC NsiI 3786 ATGCAT ATGCA^T PflMI 438 CCAGTGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 1074 CACGTG CAC^GTG PmlI 1074 CACGTG CAC^GTG PshAI 684 GACATCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 1633 CTGCAG CTGCA^G SacI 1233 GAGCTC GAGCT^C SapI 4767 GAAGAGC GCTCTTC(1/4) ScaI 3197 AGTACT AGT^ACT SfiI 416 GGCCTTTGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SpeI 4588 ACTAGT A^CTAGT SphI 2662 GCATGC GCATG^C Van91I 438 CCAGTGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 782 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### Acc65I 1069 GGTACC G^GTACC 1104 GGTACC AccII 1278 CGCG CG^CG 1409 CGCG Asp718I 1069 GGTACC G^GTACC 1104 GGTACC BsaBI 110 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 731 GATGAAAATC BsgI 1448 CTGCAC GTGCAG(16/14) 3909 GTGCAG BsmI 3388 GCATTC GAATGC(1/-1) 4120 GCATTC BspHI 1894 TCATGA T^CATGA 5039 TCATGA BsrBI 3374 CCGCTC CCGCTC(-3/-3) 5067 GAGCGG BsrFI 1458 ACCGGC [GA]^CCGG[CT] 3088 GCCGGC BssSI 202 CACGAG CACGAG(-5/-1) 4250 CTCGTG BstBI 121 TTCGAA TT^CGAA 4516 TTCGAA BstEII 2412 GGTGACC G^GT[ACGT]ACC 4346 GGTTACC BstPI 2412 GGTGACC G^GT[ACGT]ACC 4346 GGTTACC BstUI 1278 CGCG CG^CG 1409 CGCG Cfr10I 1458 ACCGGC [GA]^CCGG[CT] 3088 GCCGGC EcoNI 494 CCTTCGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 656 CCTTAAAGAGG EcoO65I 2412 GGTGACC G^GT[ACGT]ACC 4346 GGTTACC HaeII 1371 AGCGCC [GA]GCGC^[CT] 2877 AGCGCC HindIII 2463 AAGCTT A^AGCTT 5417 AAGCTT HpaI 360 GTTAAC GTT^AAC 1683 GTTAAC KpnI 1069 GGTACC GGTAC^C 1104 GGTACC MamI 110 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 731 GATGAAAATC MvnI 1278 CGCG CG^CG 1409 CGCG NcoI 1540 CCATGG C^CATGG 3346 CCATGG NspV 121 TTCGAA TT^CGAA 4516 TTCGAA RcaI 1894 TCATGA T^CATGA 5039 TCATGA SfuI 121 TTCGAA TT^CGAA 4516 TTCGAA XbaI 692 TCTAGA T^CTAGA 1649 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 116 enzymes) # ######################################### AciI AcsI AcyI AfaI AluI AlwI ApoI Asp700I AspHI AspI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaHI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI Bsp1286I Bsp1407I BspMI BsrDI BsrGI BsrI BssKI BstF5I BstNI BstXI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DraIII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflFI PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XcmI XmnI