################################## # NO CUT ENZYMES ( 63 enzymes) # ################################## AatII AccI AclI AgeI AhdI Aor51HI ApaI AscI AseI AsnI AspEI BbrPI BbvCI BglII BsaAI BsiWI BspDI BspHI BspLU11I BsrBI BsrFI BssSI Bst1107I BstBI BstZ17I Cfr10I ClaI CpoI DraIII Eam1105I Eco47III EcoT22I FseI HincII HindII HpaI NaeI NdeI NgoMIV NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI SfiI SfuI SgrAI SnaBI Sse8387I StuI SwaI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### Acc65I 4953 GGTACC G^GTACC AccIII 2186 TCCGGA T^CCGGA AflIII 1790 ACGCGT A^C[GA][CT]GT Asp718I 4953 GGTACC G^GTACC AviII 2416 TGCGCA TGC^GCA AvrII 1428 CCTAGG C^CTAGG BalI 4604 TGGCCA TGG^CCA BamHI 174 GGATCC G^GATCC BbeI 273 GGCGCC GGCGC^C BcgI 296 GCAGGCAGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 403 TGATCA T^GATCA BlnI 1428 CCTAGG C^CTAGG BlpI 602 GCTTAGC GC^T[ACGT]AGC Bpu1102I 602 GCTTAGC GC^T[ACGT]AGC BsaBI 3983 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 1845 GAGACC GGTCTC(1/5) BseAI 2186 TCCGGA T^CCGGA BsiEI 364 CGGCCG CG[GA][CT]^CG BsmBI 2379 GAGACG CGTCTC(1/5) Bsp1407I 4256 TGTACA T^GTACA BspEI 2186 TCCGGA T^CCGGA BsrGI 4256 TGTACA T^GTACA BssHII 230 GCGCGC G^CGCGC BstEII 1457 GGTGACC G^GT[ACGT]ACC BstPI 1457 GGTGACC G^GT[ACGT]ACC CelII 602 GCTTAGC GC^T[ACGT]AGC DrdI 936 GACCTTACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 364 CGGCCG C^GGCCG EclXI 364 CGGCCG C^GGCCG Eco52I 364 CGGCCG C^GGCCG EcoNI 736 CCTTTCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1457 GGTGACC G^GT[ACGT]ACC EcoRI 4870 GAATTC G^AATTC FbaI 403 TGATCA T^GATCA FspI 2416 TGCGCA TGC^GCA KasI 273 GGCGCC G^GCGCC KpnI 4953 GGTACC GGTAC^C MaeII 1411 ACGT A^CGT MamI 3983 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1824 CAATTG C^AATTG MluI 1790 ACGCGT A^CGCGT MluNI 4604 TGGCCA TGG^CCA MroI 2186 TCCGGA T^CCGGA MscI 4604 TGGCCA TGG^CCA MunI 1824 CAATTG C^AATTG NarI 273 GGCGCC GG^CGCC NcoI 1438 CCATGG C^CATGG NheI 4172 GCTAGC G^CTAGC PaeR7I 1685 CTCGAG C^TCGAG PflMI 3594 CCATTTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 3707 AGGTCCT [GA]G^G[AT]CC[CT] SacI 2217 GAGCTC GAGCT^C SexAI 4031 ACCAGGT A^CC[AT]GGT SfoI 273 GGCGCC GGC^GCC SpeI 3446 ACTAGT A^CTAGT SspBI 4256 TGTACA T^GTACA SspI 3564 AATATT AAT^ATT Van91I 3594 CCATTTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 4089 TCTAGA T^CTAGA XhoI 1685 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AcyI 273 GGCGCC G[GA]^CG[CT]C 3149 GACGCC AlwNI 928 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG 3013 CAGACTCTG AocI 1256 CCTCAGG CC^T[ACGT]AGG 4433 CCTAAGG Asp700I 648 GAAGGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3422 GAATGTTTTC AspI 411 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC 2381 GACGCTGTC BciVI 885 GTATCC GTATCC(6/5) 1348 GTATCC BpmI 291 CTGGAG CTGGAG(16/14) 2278 CTGGAG BsaHI 273 GGCGCC G[GA]^CG[CT]C 3149 GACGCC BsaWI 1933 ACCGGA [AT]^CCGG[AT] 2186 TCCGGA BsmI 2012 GAATGC GAATGC(1/-1) 3678 GCATTC BsrDI 2308 GCAATG GCAATG(2/0) 5167 GCAATG BstAPI 1985 GCAGGGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3012 GCAGACTCTGC Bsu36I 1256 CCTCAGG CC^T[ACGT]AGG 4433 CCTAAGG DraII 2651 GGGGCCT [GA]G^G[ACGT]CC[CT] 3707 AGGTCCT Eco81I 1256 CCTCAGG CC^T[ACGT]AGG 4433 CCTAAGG EcoO109I 2651 GGGGCCT [GA]G^G[ACGT]CC[CT] 3707 AGGTCCT EcoRV 807 GATATC GAT^ATC 829 GATATC Hin1I 273 GGCGCC G[GA]^CG[CT]C 3149 GACGCC HindIII 3412 AAGCTT A^AGCTT 4039 AAGCTT KspI 178 CCGCGG CCGC^GG 251 CCGCGG PflFI 411 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC 2381 GACGCTGTC PstI 1995 CTGCAG CTGCA^G 4661 CTGCAG PvuII 1909 CAGCTG CAG^CTG 4354 CAGCTG SacII 178 CCGCGG CCGC^GG 251 CCGCGG ScaI 4639 AGTACT AGT^ACT 4729 AGTACT SphI 223 GCATGC GCATG^C 553 GCATGC Tth111I 411 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC 2381 GACGCTGTC XcmI 1802 CCAGTCCCGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2739 CCACAGAAATCGTGG XmnI 648 GAAGGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3422 GAATGTTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 106 enzymes) # ######################################### AccII AciI AcsI AfaI AflII AluI Alw44I AlwI ApaLI ApoI AspHI AvaI AvaII BanI BanII BbsI BbvI BcnI BfaI BfrI BglI BmyI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DsaI EaeI EarI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmaCI XmaI