################################## # NO CUT ENZYMES ( 79 enzymes) # ################################## AatII Acc65I AccIII AclI AflIII AgeI AhdI Aor51HI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BcgI BlnI BlpI Bpu1102I BsaWI BseAI BsiEI BsiWI BspDI BspEI BspLU11I BsrBI BssHII Bst1107I BstEII BstPI BstZ17I CelII ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRV FauI FseI FspI KpnI MfeI MluI MroI MunI NdeI NheI NotI NruI PacI PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SalI SapI SbfI SexAI SfiI SgrAI SmaI SnaBI SpeI Sse8387I SwaI Tth111I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AflII 926 CTTAAG C^TTAAG BbrPI 575 CACGTG CAC^GTG BbvCI 3167 CCTCAGC CCTCAGC(-5/-2) BciVI 287 GTATCC GTATCC(6/5) BfrI 926 CTTAAG C^TTAAG BglI 4385 GCCACGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 1555 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmBI 740 GAGACG CGTCTC(1/5) Bsp1407I 1203 TGTACA T^GTACA BsrFI 2571 GCCGGC [GA]^CCGG[CT] BsrGI 1203 TGTACA T^GTACA BstBI 2055 TTCGAA TT^CGAA Cfr10I 2571 GCCGGC [GA]^CCGG[CT] DraIII 2345 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG EcoRI 2386 GAATTC G^AATTC EcoT22I 4134 ATGCAT ATGCA^T HpaI 3337 GTTAAC GTT^AAC KspI 48 CCGCGG CCGC^GG MamI 1555 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NaeI 2571 GCCGGC GCC^GGC NgoMIV 2571 GCCGGC G^CCGGC NsiI 4134 ATGCAT ATGCA^T NspI 4132 GCATGC [GA]CATG^[CT] NspV 2055 TTCGAA TT^CGAA PmaCI 575 CACGTG CAC^GTG PmlI 575 CACGTG CAC^GTG PpuMI 2493 AGGTCCT [GA]G^G[AT]CC[CT] SacII 48 CCGCGG CCGC^GG ScaI 2379 AGTACT AGT^ACT SfuI 2055 TTCGAA TT^CGAA SphI 4132 GCATGC GCATG^C SspBI 1203 TGTACA T^GTACA SspI 3368 AATATT AAT^ATT XbaI 2470 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### ApaI 549 GGGCCC GGGCC^C 2335 GGGCCC Asp700I 1507 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3117 GAAGTGCTTC BalI 156 TGGCCA TGG^CCA 4256 TGGCCA BclI 2928 TGATCA T^GATCA 4835 TGATCA BsaAI 575 CACGTG [CT]AC^GT[GA] 2150 CACGTA BspHI 993 TCATGA T^CATGA 3288 TCATGA BspMI 1590 ACCTGC ACCTGC(4/8) 622 GCAGGT BsrDI 415 CATTGC GCAATG(2/0) 2403 GCAATG BssSI 2252 CACGAG CACGAG(-5/-1) 4387 CACGAG BstAPI 1672 GCAGTATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1870 GCAGTCACTGC EcoNI 1477 CCTGCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2614 CCTCCCCAAGG FbaI 2928 TGATCA T^GATCA 4835 TGATCA HgaI 2 GCGTC GACGC(5/10) 2115 GCGTC HindIII 1051 AAGCTT A^AGCTT 3405 AAGCTT MluNI 156 TGGCCA TGG^CCA 4256 TGGCCA MscI 156 TGGCCA TGG^CCA 4256 TGGCCA PaeR7I 849 CTCGAG C^TCGAG 2832 CTCGAG RcaI 993 TCATGA T^CATGA 3288 TCATGA XhoI 849 CTCGAG C^TCGAG 2832 CTCGAG XmnI 1507 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3117 GAAGTGCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 125 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AluI Alw44I AlwI AlwNI AocI ApaLI ApoI AspHI AvaI AvaII BamHI BanI BanII BbeI BbsI BbvI BcnI BfaI BglII BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NcoI NdeII NlaIII NlaIV PflMI PleI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII