################################## # NO CUT ENZYMES ( 71 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AgeI Aor51HI AscI AseI AsnI Asp718I AspI AviII BbeI BglII BsaHI BseAI BsiEI BsmBI BspDI BspEI BspHI BssHII BstAPI ClaI CpoI DraIII EagI EclXI Eco47III Eco52I FseI FspI Hin1I HincII HindII HpaI KasI KpnI KspI MluI MroI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SfiI SfoI SgrAI SmaI Sse8387I SwaI Tth111I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccII 1659 CGCG CG^CG AflII 3229 CTTAAG C^TTAAG AhdI 2031 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 3863 GTGCAC G^TGCAC ApaLI 3863 GTGCAC G^TGCAC AspEI 2031 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BanI 2580 GGCACC G^G[CT][GA]CC BbvCI 720 GCTGAGG CCTCAGC(-5/-2) BcgI 487 GCATTCTTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 2245 GTATCC GTATCC(6/5) BclI 4355 TGATCA T^GATCA BfrI 3229 CTTAAG C^TTAAG BglI 3306 GCCTTACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 4409 GCTGAGC GC^T[ACGT]AGC Bpu1102I 4409 GCTGAGC GC^T[ACGT]AGC BsiWI 1031 CGTACG C^GTACG BspLU11I 3133 ACATGT A^CATGT BsrBI 5140 CCGCTC CCGCTC(-3/-3) BsrDI 4106 GCAATG GCAATG(2/0) BsrFI 1169 GCCGGT [GA]^CCGG[CT] Bst1107I 3109 GTATAC GTA^TAC BstBI 495 TTCGAA TT^CGAA BstEII 1172 GGTTACC G^GT[ACGT]ACC BstPI 1172 GGTTACC G^GT[ACGT]ACC BstUI 1659 CGCG CG^CG BstZ17I 3109 GTATAC GTA^TAC CelII 4409 GCTGAGC GC^T[ACGT]AGC Cfr10I 1169 GCCGGT [GA]^CCGG[CT] Eam1105I 2031 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 1172 GGTTACC G^GT[ACGT]ACC EcoRV 974 GATATC GAT^ATC FbaI 4355 TGATCA T^GATCA HaeII 39 GGCGCT [GA]GCGC^[CT] HgaI 4644 GCGTC GACGC(5/10) MvnI 1659 CGCG CG^CG NcoI 4247 CCATGG C^CATGG NspV 495 TTCGAA TT^CGAA PaeR7I 5143 CTCGAG C^TCGAG SapI 2829 GCTCTTC GCTCTTC(1/4) ScaI 92 AGTACT AGT^ACT SfuI 495 TTCGAA TT^CGAA SpeI 3237 ACTAGT A^CTAGT XcmI 189 CCATGTGGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 5143 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AocI 3391 CCTAAGG CC^T[ACGT]AGG 3919 CCTCAGG ApaI 3870 GGGCCC GGGCC^C 4461 GGGCCC AvaI 1883 CTCGGG C^[CT]CG[GA]G 5143 CTCGAG AvrII 536 CCTAGG C^CTAGG 4583 CCTAGG BalI 322 TGGCCA TGG^CCA 4373 TGGCCA BamHI 150 GGATCC G^GATCC 4595 GGATCC BbrPI 234 CACGTG CAC^GTG 3213 CACGTG BlnI 536 CCTAGG C^CTAGG 4583 CCTAGG BsaBI 507 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4391 GATTTTTATC BsaWI 1684 ACCGGA [AT]^CCGG[AT] 2163 ACCGGA BsoBI 1883 CTCGGG C^[CT]CG[GA]G 5143 CTCGAG Bsp1407I 2747 TGTACA T^GTACA 4748 TGTACA BsrGI 2747 TGTACA T^GTACA 4748 TGTACA BssSI 283 CACGAG CACGAG(-5/-1) 4804 CACGAG Bsu36I 3391 CCTAAGG CC^T[ACGT]AGG 3919 CCTCAGG DrdI 3947 GACTTTACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4022 GACTGTGAGGTC DsaI 2041 CCGTGG C^C[GA][CT]GG 4247 CCATGG Eco81I 3391 CCTAAGG CC^T[ACGT]AGG 3919 CCTCAGG EcoRI 215 GAATTC G^AATTC 3425 GAATTC EcoT22I 3666 ATGCAT ATGCA^T 3826 ATGCAT FauI 3974 CCCGC CCCGC(4/6) 2378 GCGGG MamI 507 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4391 GATTTTTATC MfeI 2680 CAATTG C^AATTG 5177 CAATTG MluNI 322 TGGCCA TGG^CCA 4373 TGGCCA MscI 322 TGGCCA TGG^CCA 4373 TGGCCA MunI 2680 CAATTG C^AATTG 5177 CAATTG NsiI 3666 ATGCAT ATGCA^T 3826 ATGCAT PmaCI 234 CACGTG CAC^GTG 3213 CACGTG PmlI 234 CACGTG CAC^GTG 3213 CACGTG SacI 56 GAGCTC GAGCT^C 264 GAGCTC SspBI 2747 TGTACA T^GTACA 4748 TGTACA SspI 1840 AATATT AAT^ATT 2053 AATATT StuI 317 AGGCCT AGG^CCT 3368 AGGCCT XbaI 867 TCTAGA T^CTAGA 5027 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 109 enzymes) # ######################################### AccI AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI Asp700I AspHI AvaII BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII EaeI EarI EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmlI SnaBI SphI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI