##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AclI AcsI AflII AgeI ApoI
AscI AseI AsnI Asp700I AspI
AviII AvrII BfrI BlnI BsaBI
BsiWI BsmBI BspDI BssHII Bst1107I
BstBI BstZ17I ClaI CpoI DraI
EcoRI FseI FspI HpaI KspI
MamI MfeI MunI NruI NspV
PacI PflFI PinAI PmeI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI Tth111I
XbaI XmnI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
Acc65I 1878 GGTACC G^GTACC
AccI 1305 GTCTAC GT^[AC][GT]AC
Alw44I 3852 GTGCAC G^TGCAC
ApaLI 3852 GTGCAC G^TGCAC
Asp718I 1878 GGTACC G^GTACC
BclI 4200 TGATCA T^GATCA
BglII 1089 AGATCT A^GATCT
BlpI 3961 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 3961 GCTAAGC GC^T[ACGT]AGC
BsmI 1575 GAATGC GAATGC(1/-1)
BspHI 1595 TCATGA T^CATGA
BssSI 666 CACGAG CACGAG(-5/-1)
CelII 3961 GCTAAGC GC^T[ACGT]AGC
EcoNI 2070 CCTGATGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 2571 GATATC GAT^ATC
EcoT22I 1706 ATGCAT ATGCA^T
FbaI 4200 TGATCA T^GATCA
HindIII 2110 AAGCTT A^AGCTT
KpnI 1878 GGTACC GGTAC^C
MluI 2217 ACGCGT A^CGCGT
NcoI 3789 CCATGG C^CATGG
NheI 1912 GCTAGC G^CTAGC
NotI 2909 GCGGCCGC GC^GGCCGC
NsiI 1706 ATGCAT ATGCA^T
PaeR7I 256 CTCGAG C^TCGAG
PshAI 271 GACAGTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1595 TCATGA T^CATGA
SapI 3889 GCTCTTC GCTCTTC(1/4)
SexAI 2453 ACCAGGT A^CC[AT]GGT
XhoI 256 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AatII 193 GACGTC GACGT^C
364 GACGTC
AccIII 2013 TCCGGA T^CCGGA
3003 TCCGGA
Aor51HI 1257 AGCGCT AGC^GCT
1328 AGCGCT
BamHI 1811 GGATCC G^GATCC
4181 GGATCC
BseAI 2013 TCCGGA T^CCGGA
3003 TCCGGA
Bsp1407I 111 TGTACA T^GTACA
4312 TGTACA
BspEI 2013 TCCGGA T^CCGGA
3003 TCCGGA
BspLU11I 3687 ACATGT A^CATGT
4123 ACATGT
BsrDI 2465 CATTGC GCAATG(2/0)
503 GCAATG
BsrGI 111 TGTACA T^GTACA
4312 TGTACA
BstEII 994 GGTAACC G^GT[ACGT]ACC
4348 GGTCACC
BstPI 994 GGTAACC G^GT[ACGT]ACC
4348 GGTCACC
DrdI 133 GACCTCAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1275 GACCACCTCGTC
Eco47III 1257 AGCGCT AGC^GCT
1328 AGCGCT
EcoO65I 994 GGTAACC G^GT[ACGT]ACC
4348 GGTCACC
HincII 3184 GTTGAC GT[CT]^[GA]AC
3395 GTTGAC
HindII 3184 GTTGAC GT[CT]^[GA]AC
3395 GTTGAC
MroI 2013 TCCGGA T^CCGGA
3003 TCCGGA
MseI 4241 TTAA T^TAA
5006 TTAA
NaeI 3227 GCCGGC GCC^GGC
4877 GCCGGC
NdeI 1376 CATATG CA^TATG
1538 CATATG
NgoMIV 3227 GCCGGC G^CCGGC
4877 GCCGGC
SacI 2995 GAGCTC GAGCT^C
3676 GAGCTC
SfiI 3346 GGCCGAGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
4902 GGCCTCTGAGGCC
SmaI 2160 CCCGGG CCC^GGG
4032 CCCGGG
SmlI 256 CTCGAG C^T[CT][GA]AG
3584 CTCAAG
SspBI 111 TGTACA T^GTACA
4312 TGTACA
Tru9I 4241 TTAA T^TAA
5006 TTAA
Tsp509I 4497 AATT ^AATT
4599 AATT
XmaCI 2160 CCCGGG C^CCGGG
4032 CCCGGG
XmaI 2160 CCCGGG C^CCGGG
4032 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 140 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AhdI AluI AlwI AlwNI AocI
ApaI AspEI AspHI AvaI AvaII
BalI BanI BanII BbeI BbrPI
BbsI BbvCI BbvI BcgI BciVI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EagI Eam1105I
EarI EclXI Eco52I Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PmaCI PmlI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SphI StuI
StyI TaqI TfiI TseI Tsp45I
TspRI TthHB8I Van91I XcmI XhoII