################################## # NO CUT ENZYMES ( 57 enzymes) # ################################## AatII AccI AclI AgeI AscI AseI AsnI AspI AvrII BcgI BciVI BglII BlnI BsiWI Bsp1407I BspDI BspHI BspLU11I BsrDI BsrGI BssHII Bst1107I BstBI BstZ17I ClaI CpoI DrdI EagI EclXI Eco52I FseI HpaI KspI MluI NdeI NheI NotI NruI NspV PacI PflFI PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SfuI SgrAI Sse8387I SspBI SwaI Tth111I ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### Acc65I 5904 GGTACC G^GTACC AflIII 5251 ACACGT A^C[GA][CT]GT Alw44I 6188 GTGCAC G^TGCAC AocI 5847 CCTAAGG CC^T[ACGT]AGG ApaLI 6188 GTGCAC G^TGCAC Asp718I 5904 GGTACC G^GTACC BbrPI 5252 CACGTG CAC^GTG BsaBI 1692 GATCCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 6757 CGACCG CG[GA][CT]^CG BstEII 6124 GGTGACC G^GT[ACGT]ACC BstPI 6124 GGTGACC G^GT[ACGT]ACC Bsu36I 5847 CCTAAGG CC^T[ACGT]AGG DraI 1920 TTTAAA TTT^AAA DraIII 1648 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 5847 CCTAAGG CC^T[ACGT]AGG EcoO65I 6124 GGTGACC G^GT[ACGT]ACC EcoRV 1933 GATATC GAT^ATC EcoT22I 60 ATGCAT ATGCA^T HindIII 6800 AAGCTT A^AGCTT KpnI 5904 GGTACC GGTAC^C MamI 1692 GATCCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 5679 CAATTG C^AATTG MunI 5679 CAATTG C^AATTG NaeI 5396 GCCGGC GCC^GGC NcoI 557 CCATGG C^CATGG NgoMIV 5396 GCCGGC G^CCGGC NsiI 60 ATGCAT ATGCA^T PmaCI 5252 CACGTG CAC^GTG PmlI 5252 CACGTG CAC^GTG PshAI 5149 GACGAGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC ScaI 6612 AGTACT AGT^ACT SexAI 5952 ACCTGGT A^CC[AT]GGT SfiI 4281 GGCCATGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SnaBI 790 TACGTA TAC^GTA SpeI 5097 ACTAGT A^CTAGT ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccIII 308 TCCGGA T^CCGGA 1694 TCCGGA AflII 4354 CTTAAG C^TTAAG 5630 CTTAAG AhdI 2347 GACCTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2947 GACTTTGAGTC ApaI 2462 GGGCCC GGGCC^C 4040 GGGCCC AspEI 2347 GACCTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2947 GACTTTGAGTC AviII 5647 TGCGCA TGC^GCA 6712 TGCGCA BbeI 4766 GGCGCC GGCGC^C 5399 GGCGCC BclI 4499 TGATCA T^GATCA 6015 TGATCA BfrI 4354 CTTAAG C^TTAAG 5630 CTTAAG BsaAI 790 TACGTA [CT]AC^GT[GA] 5252 CACGTG BseAI 308 TCCGGA T^CCGGA 1694 TCCGGA BsmI 1183 GAATGC GAATGC(1/-1) 6550 GCATTC BspEI 308 TCCGGA T^CCGGA 1694 TCCGGA BstXI 1556 CCAAGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3176 CCACAGCGCTGG DsaI 557 CCATGG C^C[GA][CT]GG 6760 CCGTGG Eam1105I 2347 GACCTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2947 GACTTTGAGTC EcoNI 1050 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4044 CCTTGAGGAGG FbaI 4499 TGATCA T^GATCA 6015 TGATCA FspI 5647 TGCGCA TGC^GCA 6712 TGCGCA KasI 4766 GGCGCC G^GCGCC 5399 GGCGCC MroI 308 TCCGGA T^CCGGA 1694 TCCGGA MslI 1464 CACTGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2085 CACGAAGGTG NarI 4766 GGCGCC GG^CGCC 5399 GGCGCC SfoI 4766 GGCGCC GGC^GCC 5399 GGCGCC SspI 196 AATATT AAT^ATT 6396 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 141 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI Aor51HI ApoI Asp700I AspHI AvaI AvaII BalI BamHI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EarI Eco47III EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PaeR7I PflMI PleI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI SphI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI