################################## # NO CUT ENZYMES ( 112 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AcyI AflII AflIII AgeI AhdI Alw44I ApaI ApaLI AscI AseI AsnI Asp718I AspEI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BfrI BglII BlnI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BspDI BspEI BspLU11I BsrBI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I EcoO65I EcoRI EcoRV EcoT22I FseI FspI Hin1I KasI KpnI KspI MfeI MluI MroI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AclI 456 AACGTT AA^CGTT AcsI 724 GAATTT [GA]^AATT[CT] AocI 1873 CCTCAGG CC^T[ACGT]AGG Aor51HI 1780 AGCGCT AGC^GCT ApoI 724 GAATTT [GA]^AATT[CT] AspI 82 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 396 CCCGAG C^[CT]CG[GA]G BanI 625 GGCACC G^G[CT][GA]CC BclI 593 TGATCA T^GATCA BcnI 853 CCCGG CC^[GC]GG BglI 1209 GCCAGGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 606 TACGTG [CT]AC^GT[GA] BsaBI 968 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmI 1975 GAATGC GAATGC(1/-1) BsoBI 396 CCCGAG C^[CT]CG[GA]G BspHI 2085 TCATGA T^CATGA BspMI 1698 ACCTGC ACCTGC(4/8) BsrDI 138 CATTGC GCAATG(2/0) BsrFI 622 ACCGGC [GA]^CCGG[CT] Bsu36I 1873 CCTCAGG CC^T[ACGT]AGG CfoI 1781 GCGC GCG^C Cfr10I 622 ACCGGC [GA]^CCGG[CT] Eco47III 1780 AGCGCT AGC^GCT Eco81I 1873 CCTCAGG CC^T[ACGT]AGG FbaI 593 TGATCA T^GATCA HaeII 1780 AGCGCT [GA]GCGC^[CT] HhaI 1781 GCGC GCG^C HinP1I 1781 GCGC G^CGC HindIII 690 AAGCTT A^AGCTT HpaI 2122 GTTAAC GTT^AAC MamI 968 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NciI 853 CCCGG CC^[GC]GG NcoI 2003 CCATGG C^CATGG PflFI 82 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC Psp1406I 456 AACGTT AA^CGTT RcaI 2085 TCATGA T^CATGA TaqI 779 TCGA T^CGA Tth111I 82 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 779 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### BalI 179 TGGCCA TGG^CCA 1679 TGGCCA BlpI 1253 GCTCAGC GC^T[ACGT]AGC 1990 GCTGAGC Bpu1102I 1253 GCTCAGC GC^T[ACGT]AGC 1990 GCTGAGC BsaI 1905 GAGACC GGTCTC(1/5) 2007 GGTCTC Bsp1407I 874 TGTACA T^GTACA 2323 TGTACA BsrGI 874 TGTACA T^GTACA 2323 TGTACA CelII 1253 GCTCAGC GC^T[ACGT]AGC 1990 GCTGAGC DsaI 286 CCGTGG C^C[GA][CT]GG 2003 CCATGG EaeI 179 TGGCCA [CT]^GGCC[GA] 1679 TGGCCA EcoNI 1430 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1947 CCTTCACCAGG EcoT14I 823 CCAAGG C^C[AT][AT]GG 2003 CCATGG HapII 623 CCGG C^CGG 854 CCGG HgaI 82 GACGC GACGC(5/10) 540 GACGC HincII 1843 GTTGAC GT[CT]^[GA]AC 2122 GTTAAC HindII 1843 GTTGAC GT[CT]^[GA]AC 2122 GTTAAC HpaII 623 CCGG C^CGG 854 CCGG MluNI 179 TGGCCA TGG^CCA 1679 TGGCCA MscI 179 TGGCCA TGG^CCA 1679 TGGCCA MslI 1151 CACTCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2038 CACTTCTGTG MspI 623 CCGG C^CGG 854 CCGG PflMI 1037 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1508 CCAGGAAATGG PleI 451 GACTC GAGTC(4/5) 2017 GAGTC PpuMI 1830 GGGACCT [GA]G^G[AT]CC[CT] 1915 GGGACCC SacI 1251 GAGCTC GAGCT^C 1449 GAGCTC SspBI 874 TGTACA T^GTACA 2323 TGTACA StuI 400 AGGCCT AGG^CCT 1427 AGGCCT StyI 823 CCAAGG C^C[AT][AT]GG 2003 CCATGG Tsp45I 1753 GTGAC ^GT[GC]AC 2101 GTCAC Van91I 1037 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1508 CCAGGAAATGG XbaI 1316 TCTAGA T^CTAGA 2028 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 77 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI Asp700I AspHI AvaII BanII BbsI BbvI BfaI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MvaI MwoI NdeII NlaIII NlaIV PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI TfiI Tru9I TseI Tsp509I TspRI XcmI XhoII XmnI