################################## # NO CUT ENZYMES ( 84 enzymes) # ################################## AccII AflII AgeI AhdI AocI Aor51HI AscI AspEI AviII AvrII BbeI BbrPI BbvCI BciVI BclI BcnI BfrI BlnI BsiEI BsiWI BspHI BsrBI BsrFI BssHII BssSI BstEII BstPI BstUI Bsu36I Cfr10I CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoT22I FauI FbaI FseI FspI KasI KspI MluI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI PacI PinAI PmaCI PmeI PmlI PpuMI PshAI PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfoI SgrAI SmaI SnaBI SphI Sse8387I SwaI XbaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AatII 1099 GACGTC GACGT^C Acc65I 550 GGTACC G^GTACC AccI 2868 GTATAC GT^[AC][GT]AC AccIII 1754 TCCGGA T^CCGGA AclI 2357 AACGTT AA^CGTT Asp718I 550 GGTACC G^GTACC AspI 1624 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 2124 GGATCC G^GATCC BcgI 2493 GCACGATCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 1581 AGATCT A^GATCT BsaAI 1597 TACGTG [CT]AC^GT[GA] BseAI 1754 TCCGGA T^CCGGA BsmBI 2449 GAGACG CGTCTC(1/5) BsmI 1497 GAATGC GAATGC(1/-1) Bsp1407I 989 TGTACA T^GTACA BspDI 2081 ATCGAT AT^CGAT BspEI 1754 TCCGGA T^CCGGA BsrGI 989 TGTACA T^GTACA Bst1107I 2868 GTATAC GTA^TAC BstBI 3299 TTCGAA TT^CGAA BstZ17I 2868 GTATAC GTA^TAC CfoI 3995 GCGC GCG^C ClaI 2081 ATCGAT AT^CGAT DraI 2983 TTTAAA TTT^AAA DraII 2636 AGGGCCC [GA]G^G[ACGT]CC[CT] EcoO109I 2636 AGGGCCC [GA]G^G[ACGT]CC[CT] EcoRI 4518 GAATTC G^AATTC HaeII 3994 GGCGCT [GA]GCGC^[CT] HhaI 3995 GCGC GCG^C HinP1I 3995 GCGC G^CGC HpaI 1785 GTTAAC GTT^AAC KpnI 550 GGTACC GGTAC^C MroI 1754 TCCGGA T^CCGGA NspV 3299 TTCGAA TT^CGAA PaeR7I 2501 CTCGAG C^TCGAG PflFI 1624 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC Psp1406I 2357 AACGTT AA^CGTT SapI 1867 GAAGAGC GCTCTTC(1/4) SfiI 2638 GGCCCACATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfuI 3299 TTCGAA TT^CGAA SpeI 1555 ACTAGT A^CTAGT SspBI 989 TGTACA T^GTACA StuI 2657 AGGCCT AGG^CCT Tth111I 1624 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 2501 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AcyI 1099 GACGTC G[GA]^CG[CT]C 2719 GACGCC Alw44I 256 GTGCAC G^TGCAC 534 GTGCAC ApaLI 256 GTGCAC G^TGCAC 534 GTGCAC AseI 556 ATTAAT AT^TAAT 1588 ATTAAT AsnI 556 ATTAAT AT^TAAT 1588 ATTAAT Asp700I 1965 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4259 GAATTTATTC AvaI 440 CTCGGG C^[CT]CG[GA]G 2501 CTCGAG BglI 280 GCCAAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2639 GCCCACATGGC BlpI 1936 GCTGAGC GC^T[ACGT]AGC 2311 GCTCAGC Bpu1102I 1936 GCTGAGC GC^T[ACGT]AGC 2311 GCTCAGC BsaHI 1099 GACGTC G[GA]^CG[CT]C 2719 GACGCC BsaWI 1754 TCCGGA [AT]^CCGG[AT] 2802 ACCGGA BseRI 447 CTCCTC GAGGAG(10/8) 1318 CTCCTC BsoBI 440 CTCGGG C^[CT]CG[GA]G 2501 CTCGAG BspLU11I 2875 ACATGT A^CATGT 4117 ACATGT BstXI 2209 CCAACAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3378 CCATGGAGGTGG CelII 1936 GCTGAGC GC^T[ACGT]AGC 2311 GCTCAGC DsaI 996 CCATGG C^C[GA][CT]GG 3378 CCATGG EcoRV 1758 GATATC GAT^ATC 3631 GATATC EcoT14I 996 CCATGG C^C[AT][AT]GG 3378 CCATGG HapII 1755 CCGG C^CGG 2803 CCGG Hin1I 1099 GACGTC G[GA]^CG[CT]C 2719 GACGCC HindIII 460 AAGCTT A^AGCTT 4388 AAGCTT HpaII 1755 CCGG C^CGG 2803 CCGG MspI 1755 CCGG C^CGG 2803 CCGG NcoI 996 CCATGG C^CATGG 3378 CCATGG NspI 2875 ACATGT [GA]CATG^[CT] 4117 ACATGT PshBI 556 ATTAAT AT^TAAT 1588 ATTAAT PvuII 1119 CAGCTG CAG^CTG 2339 CAGCTG SacI 1330 GAGCTC GAGCT^C 1926 GAGCTC SspI 2443 AATATT AAT^ATT 3469 AATATT StyI 996 CCATGG C^C[AT][AT]GG 3378 CCATGG XmnI 1965 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4259 GAATTTATTC ######################################### # 3 OR MORE CUT ENZYMES ( 96 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI ApaI ApoI AspHI AvaII BalI BanI BanII BbsI BbvI BfaI BmyI BpmI BpuAI BsaBI BsaI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EaeI EarI EcoNI EcoRII Fnu4HI FokI HaeIII HgaI HincII HindII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MfeI MflI MluNI MnlI MscI MseI MslI MspA1I MunI MvaI MwoI NdeII NlaIII NlaIV PflMI PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII