################################## # NO CUT ENZYMES ( 59 enzymes) # ################################## Acc65I AccIII AgeI AhdI Alw44I ApaLI AscI Asp718I AspEI AviII AvrII BalI BlnI BseAI BsiEI BsiWI BsmBI BspDI BspEI BsrBI BssHII ClaI CpoI EagI Eam1105I EclXI Eco52I EcoRI FseI FspI KpnI KspI MluI MluNI MroI MscI NheI NotI NruI PacI PaeR7I PinAI PmeI PshAI PvuI RsrII SacII SalI SbfI SexAI SfiI SgrAI SnaBI SpeI Sse8387I SspI SwaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AatII 3071 GACGTC GACGT^C AclI 2365 AACGTT AA^CGTT AflII 896 CTTAAG C^TTAAG AseI 5856 ATTAAT AT^TAAT AsnI 5856 ATTAAT AT^TAAT BbeI 2039 GGCGCC GGCGC^C BbrPI 5559 CACGTG CAC^GTG BcgI 1169 CGAACAAACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 3633 TGATCA T^GATCA BfrI 896 CTTAAG C^TTAAG BglI 4174 GCCAACCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 2498 GATTTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 1305 TCCGGT [AT]^CCGG[AT] BspHI 4396 TCATGA T^CATGA BsrDI 345 CATTGC GCAATG(2/0) DsaI 2172 CCATGG C^C[GA][CT]GG FbaI 3633 TGATCA T^GATCA HpaI 4145 GTTAAC GTT^AAC KasI 2039 GGCGCC G^GCGCC MamI 2498 GATTTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1788 CAATTG C^AATTG MunI 1788 CAATTG C^AATTG NaeI 1736 GCCGGC GCC^GGC NarI 2039 GGCGCC GG^CGCC NcoI 2172 CCATGG C^CATGG NgoMIV 1736 GCCGGC G^CCGGC PmaCI 5559 CACGTG CAC^GTG PmlI 5559 CACGTG CAC^GTG PshBI 5856 ATTAAT AT^TAAT Psp1406I 2365 AACGTT AA^CGTT RcaI 4396 TCATGA T^CATGA SfoI 2039 GGCGCC GGC^GCC SmaI 3473 CCCGGG CCC^GGG StuI 4181 AGGCCT AGG^CCT XmaCI 3473 CCCGGG C^CCGGG XmaI 3473 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 42 enzymes) # ################################### AccII 1398 CGCG CG^CG 4570 CGCG AcyI 2039 GGCGCC G[GA]^CG[CT]C 3071 GACGTC AocI 3749 CCTCAGG CC^T[ACGT]AGG 6551 CCTCAGG Aor51HI 1513 AGCGCT AGC^GCT 3086 AGCGCT ApaI 1362 GGGCCC GGGCC^C 2698 GGGCCC Asp700I 1423 GAATAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1666 GAAGCAGTTC BsaAI 5079 CACGTA [CT]AC^GT[GA] 5559 CACGTG BsaHI 2039 GGCGCC G[GA]^CG[CT]C 3071 GACGTC Bsp1407I 5227 TGTACA T^GTACA 6848 TGTACA BsrFI 1736 GCCGGC [GA]^CCGG[CT] 5373 ACCGGC BsrGI 5227 TGTACA T^GTACA 6848 TGTACA Bst1107I 5269 GTATAC GTA^TAC 6838 GTATAC BstAPI 422 GCATGGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3374 GCACGTCCTGC BstBI 51 TTCGAA TT^CGAA 1167 TTCGAA BstEII 1282 GGTGACC G^GT[ACGT]ACC 4495 GGTAACC BstPI 1282 GGTGACC G^GT[ACGT]ACC 4495 GGTAACC BstUI 1398 CGCG CG^CG 4570 CGCG BstZ17I 5269 GTATAC GTA^TAC 6838 GTATAC Bsu36I 3749 CCTCAGG CC^T[ACGT]AGG 6551 CCTCAGG Cfr10I 1736 GCCGGC [GA]^CCGG[CT] 5373 ACCGGC DraIII 1016 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG 4035 CACTTAGTG EaeI 5365 TGGCCG [CT]^GGCC[GA] 5537 TGGCCG Eco47III 1513 AGCGCT AGC^GCT 3086 AGCGCT Eco81I 3749 CCTCAGG CC^T[ACGT]AGG 6551 CCTCAGG EcoNI 873 CCTTTGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4523 CCTACAACAGG EcoO65I 1282 GGTGACC G^GT[ACGT]ACC 4495 GGTAACC EcoRV 1010 GATATC GAT^ATC 5956 GATATC EcoT14I 1219 CCTTGG C^C[AT][AT]GG 2172 CCATGG EcoT22I 204 ATGCAT ATGCA^T 280 ATGCAT Hin1I 2039 GGCGCC G[GA]^CG[CT]C 3071 GACGTC HincII 3287 GTTGAC GT[CT]^[GA]AC 4145 GTTAAC HindII 3287 GTTGAC GT[CT]^[GA]AC 4145 GTTAAC HindIII 630 AAGCTT A^AGCTT 3907 AAGCTT MvnI 1398 CGCG CG^CG 4570 CGCG NsiI 204 ATGCAT ATGCA^T 280 ATGCAT NspV 51 TTCGAA TT^CGAA 1167 TTCGAA SacI 2261 GAGCTC GAGCT^C 5679 GAGCTC SfuI 51 TTCGAA TT^CGAA 1167 TTCGAA SphI 474 GCATGC GCATG^C 4170 GCATGC SspBI 5227 TGTACA T^GTACA 6848 TGTACA StyI 1219 CCTTGG C^C[AT][AT]GG 2172 CCATGG XmnI 1423 GAATAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1666 GAAGCAGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 121 enzymes) # ######################################### AccI AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AspI AvaI AvaII BamHI BanI BanII BbsI BbvCI BbvI BciVI BcnI BfaI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspLU11I BspMI BsrI BssKI BssSI BstF5I BstNI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DrdI EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeI NdeII NlaIII NlaIV NspI PflFI PflMI PleI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XbaI XhoII