################################## # NO CUT ENZYMES ( 57 enzymes) # ################################## AclI AgeI Alw44I Aor51HI ApaLI AscI AviII AvrII BbeI BcgI BclI BlnI BsiWI BspDI BsrBI BssHII BstBI ClaI CpoI DraIII DrdI Eco47III EcoNI EcoRI EcoRV FbaI FseI FspI HaeII KasI KspI MfeI MluI MunI NarI NheI NotI NruI NspV PacI PinAI PmeI Psp1406I RsrII SacII SbfI SexAI SfiI SfoI SfuI SgrAI SmaI Sse8387I SwaI XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### Acc65I 2929 GGTACC G^GTACC AccII 3757 CGCG CG^CG AccIII 2892 TCCGGA T^CCGGA AflII 2264 CTTAAG C^TTAAG ApaI 2769 GGGCCC GGGCC^C AseI 1420 ATTAAT AT^TAAT AsnI 1420 ATTAAT AT^TAAT Asp718I 2929 GGTACC G^GTACC BamHI 2401 GGATCC G^GATCC BbrPI 629 CACGTG CAC^GTG BbvCI 2739 CCTCAGC CCTCAGC(-5/-2) BfrI 2264 CTTAAG C^TTAAG BglI 534 GCCTGGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1087 AGATCT A^GATCT BsaWI 2892 TCCGGA [AT]^CCGG[AT] BseAI 2892 TCCGGA T^CCGGA BspEI 2892 TCCGGA T^CCGGA BspLU11I 3524 ACATGT A^CATGT BssSI 383 CACGAG CACGAG(-5/-1) Bst1107I 4270 GTATAC GTA^TAC BstUI 3757 CGCG CG^CG BstZ17I 4270 GTATAC GTA^TAC EagI 1350 CGGCCG C^GGCCG EclXI 1350 CGGCCG C^GGCCG Eco52I 1350 CGGCCG C^GGCCG EcoT22I 4458 ATGCAT ATGCA^T HpaI 4496 GTTAAC GTT^AAC KpnI 2929 GGTACC GGTAC^C MroI 2892 TCCGGA T^CCGGA MvnI 3757 CGCG CG^CG NaeI 1348 GCCGGC GCC^GGC NcoI 803 CCATGG C^CATGG NdeI 1432 CATATG CA^TATG NgoMIV 1348 GCCGGC G^CCGGC NsiI 4458 ATGCAT ATGCA^T PaeR7I 3799 CTCGAG C^TCGAG PmaCI 629 CACGTG CAC^GTG PmlI 629 CACGTG CAC^GTG PshBI 1420 ATTAAT AT^TAAT PvuI 1830 CGATCG CGAT^CG SalI 3411 GTCGAC G^TCGAC ScaI 5203 AGTACT AGT^ACT SnaBI 4621 TACGTA TAC^GTA SphI 1515 GCATGC GCATG^C StuI 3795 AGGCCT AGG^CCT XhoI 3799 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AatII 492 GACGTC GACGT^C 3391 GACGTC AlwNI 2336 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG 2813 CAGGGTCTG AocI 2341 CCTGAGG CC^T[ACGT]AGG 3004 CCTGAGG AspI 226 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 2811 GACAGGGTC BsaBI 567 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 919 GATAGACATC BsiEI 1350 CGGCCG CG[GA][CT]^CG 1830 CGATCG Bsp1407I 849 TGTACA T^GTACA 2251 TGTACA BsrDI 445 CATTGC GCAATG(2/0) 2922 CATTGC BsrFI 1348 GCCGGC [GA]^CCGG[CT] 3140 ACCGGC BsrGI 849 TGTACA T^GTACA 2251 TGTACA BstAPI 1180 GCAAACTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3877 GCAAGGGTTGC BstEII 763 GGTCACC G^GT[ACGT]ACC 2729 GGTCACC BstPI 763 GGTCACC G^GT[ACGT]ACC 2729 GGTCACC BstXI 3821 CCAGCGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3893 CCAGCACCTTGG Bsu36I 2341 CCTGAGG CC^T[ACGT]AGG 3004 CCTGAGG CfoI 3756 GCGC GCG^C 5287 GCGC Cfr10I 1348 GCCGGC [GA]^CCGG[CT] 3140 ACCGGC DsaI 803 CCATGG C^C[GA][CT]GG 2686 CCGTGG Eco81I 2341 CCTGAGG CC^T[ACGT]AGG 3004 CCTGAGG EcoO65I 763 GGTCACC G^GT[ACGT]ACC 2729 GGTCACC FauI 1394 CCCGC CCCGC(4/6) 3514 CCCGC HhaI 3756 GCGC GCG^C 5287 GCGC HinP1I 3756 GCGC G^CGC 5287 GCGC MamI 567 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 919 GATAGACATC PflFI 226 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 2811 GACAGGGTC PflMI 65 CCAAGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 240 CCACCTGCTGG PshAI 189 GACATCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2174 GACTGCTGTC PstI 2203 CTGCAG CTGCA^G 4348 CTGCAG SacI 5032 GAGCTC GAGCT^C 5381 GAGCTC SpeI 2119 ACTAGT A^CTAGT 2368 ACTAGT SspBI 849 TGTACA T^GTACA 2251 TGTACA Tth111I 226 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 2811 GACAGGGTC Van91I 65 CCAAGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 240 CCACCTGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 122 enzymes) # ######################################### AccI AciI AcsI AcyI AfaI AflIII AhdI AluI AlwI ApoI Asp700I AspEI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BciVI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I BspHI BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I CelII Cfr13I DdeI DpnII DraI DraII EaeI Eam1105I EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI Hin1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PvuII RcaI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmnI