##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AclI AflII AflIII AgeI AhdI
AscI AseI AsnI AspEI AvrII
BciVI BfrI BlnI BsaBI BsiWI
BspLU11I BssHII BssSI Bst1107I BstBI
BstZ17I CpoI Eam1105I EcoRI EcoRV
EcoT22I FseI MamI MluI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SalI SbfI ScaI SexAI
SfuI SgrAI SmaI Sse8387I SspI
SwaI Van91I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 519 GACGTC GACGT^C
Acc65I 3432 GGTACC G^GTACC
AccIII 2720 TCCGGA T^CCGGA
AcsI 4487 AAATTT [GA]^AATT[CT]
Alw44I 2766 GTGCAC G^TGCAC
Aor51HI 825 AGCGCT AGC^GCT
ApaI 57 GGGCCC GGGCC^C
ApaLI 2766 GTGCAC G^TGCAC
ApoI 4487 AAATTT [GA]^AATT[CT]
Asp700I 3653 GAATACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3432 GGTACC G^GTACC
BamHI 2471 GGATCC G^GATCC
BbrPI 261 CACGTG CAC^GTG
BclI 673 TGATCA T^GATCA
BglII 391 AGATCT A^GATCT
BseAI 2720 TCCGGA T^CCGGA
BsmI 1702 GCATTC GAATGC(1/-1)
BspDI 2489 ATCGAT AT^CGAT
BspEI 2720 TCCGGA T^CCGGA
BsrDI 2596 CATTGC GCAATG(2/0)
BsrFI 1589 GCCGGC [GA]^CCGG[CT]
Cfr10I 1589 GCCGGC [GA]^CCGG[CT]
ClaI 2489 ATCGAT AT^CGAT
DraI 4490 TTTAAA TTT^AAA
Eco47III 825 AGCGCT AGC^GCT
FbaI 673 TGATCA T^GATCA
HincII 4466 GTTAAC GT[CT]^[GA]AC
HindII 4466 GTTAAC GT[CT]^[GA]AC
HpaI 4466 GTTAAC GTT^AAC
KpnI 3432 GGTACC GGTAC^C
MfeI 952 CAATTG C^AATTG
MroI 2720 TCCGGA T^CCGGA
MunI 952 CAATTG C^AATTG
NaeI 1589 GCCGGC GCC^GGC
NgoMIV 1589 GCCGGC G^CCGGC
PmaCI 261 CACGTG CAC^GTG
PmlI 261 CACGTG CAC^GTG
SfiI 2961 GGCCACACTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SnaBI 1626 TACGTA TAC^GTA
SpeI 2871 ACTAGT A^CTAGT
XmnI 3653 GAATACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 2313 GTCTAC GT^[AC][GT]AC
2442 GTCTAC
AspI 3365 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3867 GACACTGTC
AviII 71 TGCGCA TGC^GCA
310 TGCGCA
BbeI 1592 GGCGCC GGCGC^C
3097 GGCGCC
BcgI 839 CGAGCGGCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3353 GCATACCATTCG
BsaWI 737 ACCGGA [AT]^CCGG[AT]
2720 TCCGGA
Bsp1407I 1057 TGTACA T^GTACA
3625 TGTACA
BspHI 670 TCATGA T^CATGA
1525 TCATGA
BsrGI 1057 TGTACA T^GTACA
3625 TGTACA
DraIII 453 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG
4126 CACCTTGTG
FspI 71 TGCGCA TGC^GCA
310 TGCGCA
HindIII 1649 AAGCTT A^AGCTT
3508 AAGCTT
KasI 1592 GGCGCC G^GCGCC
3097 GGCGCC
KspI 16 CCGCGG CCGC^GG
223 CCGCGG
NarI 1592 GGCGCC GG^CGCC
3097 GGCGCC
PflFI 3365 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3867 GACACTGTC
RcaI 670 TCATGA T^CATGA
1525 TCATGA
SacII 16 CCGCGG CCGC^GG
223 CCGCGG
SapI 2109 GAAGAGC GCTCTTC(1/4)
2188 GAAGAGC
SfoI 1592 GGCGCC GGC^GCC
3097 GGCGCC
SspBI 1057 TGTACA T^GTACA
3625 TGTACA
Tth111I 3365 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3867 GACACTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrI BssKI BstAPI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DrdI DsaI EaeI EagI EarI
EclXI Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SphI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XbaI XcmI XhoII