################################## # NO CUT ENZYMES ( 74 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI Aor51HI ApaI AscI Asp718I AspI AviII AvrII BamHI BbeI BcgI BglII BlnI BsaHI BsaI BseAI BsiWI BspEI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CpoI DraIII DrdI Eco47III EcoNI EcoO65I FseI FspI HaeII Hin1I HpaI KasI KpnI KspI MluI MroI NaeI NarI NcoI NdeI NgoMIV NheI NruI NspV PflFI PmeI Psp1406I PvuI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I SwaI Tth111I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccI 3221 GTCTAC GT^[AC][GT]AC AccII 1042 CGCG CG^CG AgeI 3258 ACCGGT A^CCGGT AhdI 3279 GACCAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 3279 GACCAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbrPI 2656 CACGTG CAC^GTG BbsI 3287 GTCTTC GAAGAC(2/6) BbvCI 1221 CCTCAGC CCTCAGC(-5/-2) BglI 3054 GCCAACAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 3287 GTCTTC GAAGAC(2/6) BsaAI 2656 CACGTG [CT]AC^GT[GA] BsaWI 3258 ACCGGT [AT]^CCGG[AT] BsiEI 2863 CGGCCG CG[GA][CT]^CG BsmBI 2100 GAGACG CGTCTC(1/5) BsmI 413 GCATTC GAATGC(1/-1) Bsp1407I 1765 TGTACA T^GTACA BsrDI 5431 CATTGC GCAATG(2/0) BsrGI 1765 TGTACA T^GTACA BssSI 1024 CACGAG CACGAG(-5/-1) BstUI 1042 CGCG CG^CG DraII 2700 GGGTCCC [GA]G^G[ACGT]CC[CT] DsaI 1966 CCACGG C^C[GA][CT]GG EagI 2863 CGGCCG C^GGCCG Eam1105I 3279 GACCAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 2863 CGGCCG C^GGCCG Eco52I 2863 CGGCCG C^GGCCG EcoO109I 2700 GGGTCCC [GA]G^G[ACGT]CC[CT] EcoRI 2228 GAATTC G^AATTC EcoRV 5069 GATATC GAT^ATC EcoT22I 160 ATGCAT ATGCA^T MvnI 1042 CGCG CG^CG NotI 2862 GCGGCCGC GC^GGCCGC NsiI 160 ATGCAT ATGCA^T PflMI 1421 CCAGCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 3258 ACCGGT A^CCGGT PmaCI 2656 CACGTG CAC^GTG PmlI 2656 CACGTG CAC^GTG PpuMI 2700 GGGTCCC [GA]G^G[AT]CC[CT] PshAI 639 GACTCATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SpeI 310 ACTAGT A^CTAGT SspBI 1765 TGTACA T^GTACA StuI 2361 AGGCCT AGG^CCT Van91I 1421 CCAGCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### AflII 239 CTTAAG C^TTAAG 2393 CTTAAG Alw44I 2659 GTGCAC G^TGCAC 3312 GTGCAC AocI 2504 CCTCAGG CC^T[ACGT]AGG 2642 CCTGAGG ApaLI 2659 GTGCAC G^TGCAC 3312 GTGCAC BanII 95 GAGCTC G[GA]GC[CT]^C 3299 GAGCTC BciVI 389 GTATCC GTATCC(6/5) 3761 GTATCC BcnI 1263 CCCGG CC^[GC]GG 1858 CCCGG BfrI 239 CTTAAG C^TTAAG 2393 CTTAAG BlpI 1527 GCTTAGC GC^T[ACGT]AGC 3342 GCTCAGC Bpu1102I 1527 GCTTAGC GC^T[ACGT]AGC 3342 GCTCAGC BsaBI 1356 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3486 GATGCTGATC BspDI 3493 ATCGAT AT^CGAT 4174 ATCGAT BspHI 3016 TCATGA T^CATGA 5416 TCATGA BspLU11I 192 ACATGT A^CATGT 1949 ACATGT BsrBI 3340 CCGCTC CCGCTC(-3/-3) 2820 GAGCGG BsrFI 2178 GCCGGT [GA]^CCGG[CT] 3258 ACCGGT Bsu36I 2504 CCTCAGG CC^T[ACGT]AGG 2642 CCTGAGG CelII 1527 GCTTAGC GC^T[ACGT]AGC 3342 GCTCAGC CfoI 1041 GCGC GCG^C 2915 GCGC Cfr10I 2178 GCCGGT [GA]^CCGG[CT] 3258 ACCGGT ClaI 3493 ATCGAT AT^CGAT 4174 ATCGAT EarI 1091 CTCTTC CTCTTC(1/4) 1474 GAAGAG Eco81I 2504 CCTCAGG CC^T[ACGT]AGG 2642 CCTGAGG EcoT14I 1999 CCAAGG C^C[AT][AT]GG 3355 CCTTGG HhaI 1041 GCGC GCG^C 2915 GCGC HinP1I 1041 GCGC G^CGC 2915 GCGC HindIII 3246 AAGCTT A^AGCTT 3936 AAGCTT Ksp632I 1091 CTCTTC CTCTTC(1/4) 1474 GAAGAG MamI 1356 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3486 GATGCTGATC NciI 1263 CCCGG CC^[GC]GG 1858 CCCGG PacI 924 TTAATTAA TTAAT^TAA 2074 TTAATTAA RcaI 3016 TCATGA T^CATGA 5416 TCATGA SacI 95 GAGCTC GAGCT^C 3299 GAGCTC SphI 399 GCATGC GCATG^C 3775 GCATGC StyI 1999 CCAAGG C^C[AT][AT]GG 3355 CCTTGG XcmI 984 CCAGTTCTAATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3361 CCATGCTGATGTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 105 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AseI AsnI Asp700I AspHI AvaI AvaII BalI BanI BbvI BclI BfaI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I Cfr13I DdeI DpnII DraI EaeI EcoRII FauI FbaI Fnu4HI FokI HaeIII HapII HgaI HincII HindII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MfeI MflI MluNI MnlI MscI MseI MslI MspA1I MspI MunI MvaI MwoI NdeII NlaIII NlaIV NspI PaeR7I PleI PshBI PstI PvuII RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SspI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XbaI XhoI XhoII XmnI