################################## # NO CUT ENZYMES ( 71 enzymes) # ################################## AatII AclI AflII AgeI Aor51HI AscI AseI AsnI AspI AviII BbeI BcgI BfrI BglII BsaBI BsiEI BspDI BspHI BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI EagI EclXI Eco47III Eco52I EcoO65I EcoRI FseI FspI HindIII KasI KspI MamI MluI NarI NdeI NotI NruI NspV PacI PaeR7I PflFI PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### Acc65I 2271 GGTACC G^GTACC AccI 4056 GTCTAC GT^[AC][GT]AC AccII 1957 CGCG CG^CG AccIII 6 TCCGGA T^CCGGA AcyI 333 GACGCC G[GA]^CG[CT]C ApaI 3147 GGGCCC GGGCC^C Asp700I 4572 GAACTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 2271 GGTACC G^GTACC BalI 27 TGGCCA TGG^CCA BamHI 1275 GGATCC G^GATCC BbrPI 3655 CACGTG CAC^GTG BciVI 2429 GGATAC GTATCC(6/5) BclI 4766 TGATCA T^GATCA BglI 1998 GCCGGAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 3655 CACGTG [CT]AC^GT[GA] BsaHI 333 GACGCC G[GA]^CG[CT]C BsaWI 6 TCCGGA [AT]^CCGG[AT] BseAI 6 TCCGGA T^CCGGA BsiWI 1482 CGTACG C^GTACG BsmBI 855 CGTCTC CGTCTC(1/5) Bsp1407I 5021 TGTACA T^GTACA BspEI 6 TCCGGA T^CCGGA BspLU11I 3716 ACATGT A^CATGT BsrDI 4252 GCAATG GCAATG(2/0) BsrFI 2223 GCCGGC [GA]^CCGG[CT] BsrGI 5021 TGTACA T^GTACA BssSI 1869 CTCGTG CACGAG(-5/-1) BstUI 1957 CGCG CG^CG Cfr10I 2223 GCCGGC [GA]^CCGG[CT] EcoNI 1303 CCTGCTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 4504 ATGCAT ATGCA^T FbaI 4766 TGATCA T^GATCA Hin1I 333 GACGCC G[GA]^CG[CT]C HpaI 3372 GTTAAC GTT^AAC KpnI 2271 GGTACC GGTAC^C MfeI 3584 CAATTG C^AATTG MluNI 27 TGGCCA TGG^CCA MroI 6 TCCGGA T^CCGGA MscI 27 TGGCCA TGG^CCA MunI 3584 CAATTG C^AATTG MvnI 1957 CGCG CG^CG NaeI 2223 GCCGGC GCC^GGC NgoMIV 2223 GCCGGC G^CCGGC NheI 4217 GCTAGC G^CTAGC NsiI 4504 ATGCAT ATGCA^T PmaCI 3655 CACGTG CAC^GTG PmlI 3655 CACGTG CAC^GTG PshAI 2754 GACTCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SapI 259 GCTCTTC GCTCTTC(1/4) ScaI 4313 AGTACT AGT^ACT SspBI 5021 TGTACA T^GTACA SwaI 5038 ATTTAAAT ATTT^AAAT XbaI 5111 TCTAGA T^CTAGA XcmI 2093 CCAGAAGGAGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 4572 GAACTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AflIII 2437 ACGTGT A^C[GA][CT]GT 3716 ACATGT AhdI 2282 GACGGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3978 GACTTCATGTC Alw44I 3652 GTGCAC G^TGCAC 3658 GTGCAC AocI 47 CCTGAGG CC^T[ACGT]AGG 3528 CCTCAGG ApaLI 3652 GTGCAC G^TGCAC 3658 GTGCAC AspEI 2282 GACGGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3978 GACTTCATGTC AvrII 79 CCTAGG C^CTAGG 3475 CCTAGG BlnI 79 CCTAGG C^CTAGG 3475 CCTAGG BlpI 769 GCTGAGC GC^T[ACGT]AGC 4161 GCTCAGC Bpu1102I 769 GCTGAGC GC^T[ACGT]AGC 4161 GCTCAGC BsgI 1067 GTGCAG GTGCAG(16/14) 1911 GTGCAG BsmI 213 GCATTC GAATGC(1/-1) 4294 GCATTC BsrBI 1745 GAGCGG CCGCTC(-3/-3) 3454 GAGCGG BstYI 1275 GGATCC [GA]^GATC[CT] 3922 GGATCT Bsu36I 47 CCTGAGG CC^T[ACGT]AGG 3528 CCTCAGG CelII 769 GCTGAGC GC^T[ACGT]AGC 4161 GCTCAGC DraIII 1038 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG 2681 CACTTTGTG DrdI 2956 GACAAAGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3800 GACAAGGCTGTC Eam1105I 2282 GACGGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3978 GACTTCATGTC Eco81I 47 CCTGAGG CC^T[ACGT]AGG 3528 CCTCAGG EcoRV 1637 GATATC GAT^ATC 1849 GATATC HaeII 228 AGCGCC [GA]GCGC^[CT] 1342 GGCGCT MflI 1275 GGATCC [GA]^GATC[CT] 3922 GGATCT MslI 2327 CACCACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2940 CATTAAAATG SacI 1430 GAGCTC GAGCT^C 3329 GAGCTC SspI 4481 AATATT AAT^ATT 4511 AATATT StuI 2153 AGGCCT AGG^CCT 3478 AGGCCT XhoII 1275 GGATCC [GA]^GATC[CT] 3922 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 104 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaI AvaII BanI BanII BbsI BbvCI BbvI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstAPI BstF5I BstNI BstXI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I