################################## # NO CUT ENZYMES ( 66 enzymes) # ################################## Acc65I AclI AflIII AgeI Aor51HI AscI Asp718I AviII AvrII BbeI BbrPI BbsI BglII BlnI BpuAI BsaAI BsiWI BsmI BspHI BspLU11I BsrDI BssHII BstBI CpoI Eco47III EcoRI EcoT22I FseI FspI HpaI KasI KpnI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SalI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI SwaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AatII 3031 GACGTC GACGT^C AflII 541 CTTAAG C^TTAAG AspI 1925 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC BcgI 3689 GCATGAGGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 541 CTTAAG C^TTAAG BglI 1182 GCCTCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 266 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 2004 CGGCCG CG[GA][CT]^CG Bsp1407I 1299 TGTACA T^GTACA BspDI 3717 ATCGAT AT^CGAT BsrGI 1299 TGTACA T^GTACA Bst1107I 2475 GTATAC GTA^TAC BstEII 2712 GGTGACC G^GT[ACGT]ACC BstPI 2712 GGTGACC G^GT[ACGT]ACC BstZ17I 2475 GTATAC GTA^TAC ClaI 3717 ATCGAT AT^CGAT EagI 2004 CGGCCG C^GGCCG EclXI 2004 CGGCCG C^GGCCG Eco52I 2004 CGGCCG C^GGCCG EcoO65I 2712 GGTGACC G^GT[ACGT]ACC KspI 1483 CCGCGG CCGC^GG MamI 266 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 2174 CAATTG C^AATTG MunI 2174 CAATTG C^AATTG PflFI 1925 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC SacII 1483 CCGCGG CCGC^GG SbfI 3200 CCTGCAGG CCTGCA^GG SexAI 3922 ACCAGGT A^CC[AT]GGT Sse8387I 3200 CCTGCAGG CCTGCA^GG SspBI 1299 TGTACA T^GTACA SspI 4364 AATATT AAT^ATT Tth111I 1925 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### AccII 1484 CGCG CG^CG 3631 CGCG AccIII 2281 TCCGGA T^CCGGA 3008 TCCGGA AcyI 1493 GACGCC G[GA]^CG[CT]C 3031 GACGTC Alw44I 635 GTGCAC G^TGCAC 3626 GTGCAC AocI 62 CCTCAGG CC^T[ACGT]AGG 932 CCTCAGG ApaLI 635 GTGCAC G^TGCAC 3626 GTGCAC AseI 4361 ATTAAT AT^TAAT 5155 ATTAAT AsnI 4361 ATTAAT AT^TAAT 5155 ATTAAT Asp700I 4023 GAAAAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4883 GAACTGTTTC BalI 1798 TGGCCA TGG^CCA 3568 TGGCCA BciVI 422 GTATCC GTATCC(6/5) 3888 GTATCC BclI 2963 TGATCA T^GATCA 4805 TGATCA BsaHI 1493 GACGCC G[GA]^CG[CT]C 3031 GACGTC BseAI 2281 TCCGGA T^CCGGA 3008 TCCGGA BspEI 2281 TCCGGA T^CCGGA 3008 TCCGGA BsrBI 1900 CCGCTC CCGCTC(-3/-3) 2838 CCGCTC BsrFI 1091 ACCGGC [GA]^CCGG[CT] 1975 ACCGGC BstUI 1484 CGCG CG^CG 3631 CGCG Bsu36I 62 CCTCAGG CC^T[ACGT]AGG 932 CCTCAGG Cfr10I 1091 ACCGGC [GA]^CCGG[CT] 1975 ACCGGC DraI 1672 TTTAAA TTT^AAA 1741 TTTAAA DraIII 2329 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG 3655 CACCTAGTG Eco81I 62 CCTCAGG CC^T[ACGT]AGG 932 CCTCAGG EcoRV 1509 GATATC GAT^ATC 5039 GATATC FbaI 2963 TGATCA T^GATCA 4805 TGATCA Hin1I 1493 GACGCC G[GA]^CG[CT]C 3031 GACGTC HindIII 1387 AAGCTT A^AGCTT 3369 AAGCTT MluNI 1798 TGGCCA TGG^CCA 3568 TGGCCA MroI 2281 TCCGGA T^CCGGA 3008 TCCGGA MscI 1798 TGGCCA TGG^CCA 3568 TGGCCA MvnI 1484 CGCG CG^CG 3631 CGCG PshBI 4361 ATTAAT AT^TAAT 5155 ATTAAT SacI 3711 GAGCTC GAGCT^C 4265 GAGCTC XbaI 2798 TCTAGA T^CTAGA 4031 TCTAGA XcmI 4275 CCAGAACGGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4303 CCAAGGTCTACCTGG XmnI 4023 GAAAAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4883 GAACTGTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 124 enzymes) # ######################################### AccI AciI AcsI AfaI AhdI AluI AlwI AlwNI ApaI ApoI AspEI AspHI AvaI AvaII BamHI BanI BanII BbvCI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BssSI BstAPI BstF5I BstNI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DrdI DsaI EaeI Eam1105I EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV PaeR7I PflMI PleI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoI XhoII