################################## # NO CUT ENZYMES ( 59 enzymes) # ################################## AatII AccIII AclI AhdI Aor51HI AscI AseI AsnI AspEI AviII AvrII BbrPI BclI BlnI BsaBI BsaI BseAI BsiWI BsmI BspDI BspEI BssHII BstBI ClaI CpoI DrdI Eam1105I Eco47III EcoRV EcoT22I FbaI FseI FspI MamI MfeI MluI MroI MunI NdeI NotI NsiI NspV PacI PmaCI PmeI PmlI PshBI Psp1406I RsrII SalI SbfI SfiI SfuI SgrAI SnaBI SpeI Sse8387I StuI SwaI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### Acc65I 2294 GGTACC G^GTACC AccI 4999 GTATAC GT^[AC][GT]AC AgeI 3115 ACCGGT A^CCGGT AocI 1464 CCTGAGG CC^T[ACGT]AGG ApaI 620 GGGCCC GGGCC^C Asp718I 2294 GGTACC G^GTACC AspI 600 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 39 GGATCC G^GATCC BbvCI 384 CCTCAGC CCTCAGC(-5/-2) BcgI 3050 CGAGTCCGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 1640 AGATCT A^GATCT BlpI 15 GCTGAGC GC^T[ACGT]AGC Bpu1102I 15 GCTGAGC GC^T[ACGT]AGC BsaAI 4258 CACGTA [CT]AC^GT[GA] BsaWI 3115 ACCGGT [AT]^CCGG[AT] BsmBI 3846 CGTCTC CGTCTC(1/5) BspHI 596 TCATGA T^CATGA BsrDI 5007 GCAATG GCAATG(2/0) BssSI 3986 CTCGTG CACGAG(-5/-1) Bst1107I 4999 GTATAC GTA^TAC BstEII 2142 GGTTACC G^GT[ACGT]ACC BstPI 2142 GGTTACC G^GT[ACGT]ACC BstXI 4949 CCATCATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 4999 GTATAC GTA^TAC Bsu36I 1464 CCTGAGG CC^T[ACGT]AGG CelII 15 GCTGAGC GC^T[ACGT]AGC DraIII 1587 CACGTTGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 1464 CCTGAGG CC^T[ACGT]AGG EcoO65I 2142 GGTTACC G^GT[ACGT]ACC EcoRI 1846 GAATTC G^AATTC HindIII 292 AAGCTT A^AGCTT HpaI 4297 GTTAAC GTT^AAC KpnI 2294 GGTACC GGTAC^C KspI 5322 CCGCGG CCGC^GG NcoI 938 CCATGG C^CATGG NruI 2389 TCGCGA TCG^CGA PaeR7I 4485 CTCGAG C^TCGAG PflFI 600 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 3115 ACCGGT A^CCGGT PshAI 996 GACTGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuI 3916 CGATCG CGAT^CG RcaI 596 TCATGA T^CATGA SacI 565 GAGCTC GAGCT^C SacII 5322 CCGCGG CCGC^GG SapI 1470 GAAGAGC GCTCTTC(1/4) ScaI 4138 AGTACT AGT^ACT SmaI 2486 CCCGGG CCC^GGG SphI 11 GCATGC GCATG^C SspI 5445 AATATT AAT^ATT Tth111I 600 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 5215 TCTAGA T^CTAGA XhoI 4485 CTCGAG C^TCGAG XmaCI 2486 CCCGGG C^CCGGG XmaI 2486 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AflII 3945 CTTAAG C^TTAAG 5111 CTTAAG Alw44I 275 GTGCAC G^TGCAC 1370 GTGCAC ApaLI 275 GTGCAC G^TGCAC 1370 GTGCAC BbeI 1234 GGCGCC GGCGC^C 3166 GGCGCC BciVI 3685 GGATAC GTATCC(6/5) 3213 GTATCC BfrI 3945 CTTAAG C^TTAAG 5111 CTTAAG Bsp1407I 2258 TGTACA T^GTACA 5366 TGTACA BsrGI 2258 TGTACA T^GTACA 5366 TGTACA EagI 1613 CGGCCG C^GGCCG 1992 CGGCCG EclXI 1613 CGGCCG C^GGCCG 1992 CGGCCG Eco52I 1613 CGGCCG C^GGCCG 1992 CGGCCG HaeII 1234 GGCGCC [GA]GCGC^[CT] 3166 GGCGCC HincII 1573 GTCAAC GT[CT]^[GA]AC 4297 GTTAAC HindII 1573 GTCAAC GT[CT]^[GA]AC 4297 GTTAAC KasI 1234 GGCGCC G^GCGCC 3166 GGCGCC MslI 3378 CATAAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 4819 CATAGTCATG NarI 1234 GGCGCC GG^CGCC 3166 GGCGCC NheI 4599 GCTAGC G^CTAGC 4850 GCTAGC PpuMI 2649 GGGTCCC [GA]G^G[AT]CC[CT] 4765 AGGACCC SexAI 515 ACCTGGT A^CC[AT]GGT 1949 ACCTGGT SfoI 1234 GGCGCC GGC^GCC 3166 GGCGCC SspBI 2258 TGTACA T^GTACA 5366 TGTACA ######################################### # 3 OR MORE CUT ENZYMES ( 123 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI ApoI Asp700I AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspLU11I BspMI BsrBI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmnI