################################## # NO CUT ENZYMES ( 68 enzymes) # ################################## AatII AccII AclI AgeI AocI Aor51HI AscI AvrII BbrPI BcgI BciVI BlnI BsaBI BsiEI BsiWI BsmBI Bsp1407I BspDI BspLU11I BsrGI BssHII BstBI BstUI Bsu36I ClaI CpoI EagI EclXI Eco47III Eco52I Eco81I EcoRV FauI HpaI KspI MamI MfeI MluI MunI MvnI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacII SbfI SfiI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AcyI 2745 GGCGCC G[GA]^CG[CT]C AflIII 3185 ACGTGT A^C[GA][CT]GT AhdI 3786 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AseI 4895 ATTAAT AT^TAAT AsnI 4895 ATTAAT AT^TAAT AspEI 3786 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 359 CCCGAG C^[CT]CG[GA]G AviII 660 TGCGCA TGC^GCA BamHI 1264 GGATCC G^GATCC BbeI 2745 GGCGCC GGCGC^C BglII 2373 AGATCT A^GATCT BlpI 328 GCTAAGC GC^T[ACGT]AGC Bpu1102I 328 GCTAAGC GC^T[ACGT]AGC BsaAI 3184 TACGTG [CT]AC^GT[GA] BsaHI 2745 GGCGCC G[GA]^CG[CT]C BsmI 3561 GAATGC GAATGC(1/-1) BsoBI 359 CCCGAG C^[CT]CG[GA]G BsrBI 619 CCGCTC CCGCTC(-3/-3) Bst1107I 647 GTATAC GTA^TAC BstEII 1800 GGTTACC G^GT[ACGT]ACC BstPI 1800 GGTTACC G^GT[ACGT]ACC BstZ17I 647 GTATAC GTA^TAC CelII 328 GCTAAGC GC^T[ACGT]AGC DraI 3190 TTTAAA TTT^AAA DrdI 2141 GACATCAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 3786 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 1723 CCTTGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1800 GGTTACC G^GT[ACGT]ACC EcoT22I 4141 ATGCAT ATGCA^T FseI 1187 GGCCGGCC GGCCGG^CC FspI 660 TGCGCA TGC^GCA HaeII 2745 GGCGCC [GA]GCGC^[CT] Hin1I 2745 GGCGCC G[GA]^CG[CT]C HincII 459 GTCGAC GT[CT]^[GA]AC HindII 459 GTCGAC GT[CT]^[GA]AC HindIII 3800 AAGCTT A^AGCTT KasI 2745 GGCGCC G^GCGCC NaeI 1188 GCCGGC GCC^GGC NarI 2745 GGCGCC GG^CGCC NgoMIV 1188 GCCGGC G^CCGGC NheI 4165 GCTAGC G^CTAGC NsiI 4141 ATGCAT ATGCA^T PshAI 3125 GACATTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 4895 ATTAAT AT^TAAT SalI 459 GTCGAC G^TCGAC SapI 2125 GAAGAGC GCTCTTC(1/4) ScaI 2040 AGTACT AGT^ACT SexAI 1630 ACCTGGT A^CC[AT]GGT SfoI 2745 GGCGCC GGC^GCC SphI 866 GCATGC GCATG^C XbaI 2245 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### Acc65I 82 GGTACC G^GTACC 3695 GGTACC AccIII 207 TCCGGA T^CCGGA 1035 TCCGGA AflII 1430 CTTAAG C^TTAAG 3422 CTTAAG Asp718I 82 GGTACC G^GTACC 3695 GGTACC AspI 185 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1640 GACGTGGTC BbvCI 802 CCTCAGC CCTCAGC(-5/-2) 2470 CCTCAGC BclI 3232 TGATCA T^GATCA 3439 TGATCA BfrI 1430 CTTAAG C^TTAAG 3422 CTTAAG BglI 824 GCCAGTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2814 GCCTTGGGGGC BseAI 207 TCCGGA T^CCGGA 1035 TCCGGA BspEI 207 TCCGGA T^CCGGA 1035 TCCGGA BspHI 3516 TCATGA T^CATGA 3650 TCATGA BsrFI 162 ACCGGC [GA]^CCGG[CT] 1188 GCCGGC BssSI 1498 CTCGTG CACGAG(-5/-1) 3501 CTCGTG BstXI 48 CCAAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1812 CCAGATCCATGG Cfr10I 162 ACCGGC [GA]^CCGG[CT] 1188 GCCGGC DraIII 2427 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG 4909 CACTCTGTG FbaI 3232 TGATCA T^GATCA 3439 TGATCA HgaI 4319 GACGC GACGC(5/10) 1706 GCGTC KpnI 82 GGTACC GGTAC^C 3695 GGTACC MroI 207 TCCGGA T^CCGGA 1035 TCCGGA NdeI 3317 CATATG CA^TATG 4604 CATATG PflFI 185 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1640 GACGTGGTC PflMI 1086 CCAGCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3789 CCAGAGTCTGG RcaI 3516 TCATGA T^CATGA 3650 TCATGA SacI 473 GAGCTC GAGCT^C 3661 GAGCTC Tth111I 185 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1640 GACGTGGTC Van91I 1086 CCAGCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3789 CCAGAGTCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 111 enzymes) # ######################################### AccI AciI AcsI AfaI AluI Alw44I AlwI AlwNI ApaI ApaLI ApoI Asp700I AspHI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmnI