##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII Acc65I AccIII AcyI AgeI
Aor51HI AscI Asp718I AviII BbeI
BbrPI BglI BglII BlpI Bpu1102I
BsaBI BsaHI BseAI BsiEI BsiWI
BspEI BsrFI BssHII BssSI Bst1107I
BstAPI BstEII BstPI BstZ17I CelII
Cfr10I CpoI EagI EclXI Eco47III
Eco52I EcoO65I EcoRV FseI FspI
HgaI Hin1I KasI KpnI MamI
MfeI MluI MroI MunI NaeI
NarI NdeI NgoMIV NotI NruI
PacI PinAI PmaCI PmeI PmlI
PshAI PvuI RsrII SalI SbfI
SexAI SfiI SfoI SgrAI SmaI
SphI Sse8387I SwaI XbaI XcmI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccI 2872 GTCTAC GT^[AC][GT]AC
AccII 5440 CGCG CG^CG
AclI 1320 AACGTT AA^CGTT
AflIII 2525 ACATGT A^C[GA][CT]GT
Alw44I 3435 GTGCAC G^TGCAC
AocI 469 CCTGAGG CC^T[ACGT]AGG
ApaI 5139 GGGCCC GGGCC^C
ApaLI 3435 GTGCAC G^TGCAC
AspI 1020 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 989 CCTAGG C^CTAGG
BbvCI 329 GCTGAGG CCTCAGC(-5/-2)
BcnI 4471 CCGGG CC^[GC]GG
BlnI 989 CCTAGG C^CTAGG
BsaAI 5167 TACGTA [CT]AC^GT[GA]
BsmBI 1359 CGTCTC CGTCTC(1/5)
BspDI 3897 ATCGAT AT^CGAT
BspLU11I 2525 ACATGT A^CATGT
BspMI 124 GCAGGT ACCTGC(4/8)
BstBI 1530 TTCGAA TT^CGAA
BstUI 5440 CGCG CG^CG
Bsu36I 469 CCTGAGG CC^T[ACGT]AGG
ClaI 3897 ATCGAT AT^CGAT
DraIII 4920 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 469 CCTGAGG CC^T[ACGT]AGG
EcoNI 4997 CCTGGCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 3346 GCGGG CCCGC(4/6)
HpaI 3608 GTTAAC GTT^AAC
KspI 5439 CCGCGG CCGC^GG
MvnI 5440 CGCG CG^CG
NciI 4471 CCGGG CC^[GC]GG
NcoI 3632 CCATGG C^CATGG
NheI 1718 GCTAGC G^CTAGC
NspV 1530 TTCGAA TT^CGAA
PaeR7I 3120 CTCGAG C^TCGAG
PflFI 1020 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3856 CCAGTTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1018 AGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 1320 AACGTT AA^CGTT
SacII 5439 CCGCGG CCGC^GG
SfuI 1530 TTCGAA TT^CGAA
SnaBI 5167 TACGTA TAC^GTA
SpeI 4831 ACTAGT A^CTAGT
StuI 706 AGGCCT AGG^CCT
Tsp45I 2942 GTCAC ^GT[GC]AC
Tth111I 1020 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3856 CCAGTTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 3120 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AhdI 1094 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2708 GACCGGATGTC
AlwNI 459 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
4610 CAGATTCTG
AseI 971 ATTAAT AT^TAAT
1828 ATTAAT
AsnI 971 ATTAAT AT^TAAT
1828 ATTAAT
AspEI 1094 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2708 GACCGGATGTC
BamHI 4040 GGATCC G^GATCC
4340 GGATCC
BanI 3159 GGTGCC G^G[CT][GA]CC
4405 GGTGCC
BbsI 1345 GAAGAC GAAGAC(2/6)
1026 GTCTTC
BciVI 547 GGATAC GTATCC(6/5)
993 GGATAC
BclI 1158 TGATCA T^GATCA
5033 TGATCA
BpuAI 1345 GAAGAC GAAGAC(2/6)
1026 GTCTTC
BsaI 378 GAGACC GGTCTC(1/5)
3716 GAGACC
BsaWI 2709 ACCGGA [AT]^CCGG[AT]
5071 ACCGGA
BspHI 414 TCATGA T^CATGA
1990 TCATGA
BsrBI 3336 GAGCGG CCGCTC(-3/-3)
4529 GAGCGG
CfoI 222 GCGC GCG^C
575 GCGC
DraII 1018 AGGACCT [GA]G^G[ACGT]CC[CT]
5139 GGGCCCT
DrdI 1348 GACAAGTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2933 GACACCGTTGTC
DsaI 3632 CCATGG C^C[GA][CT]GG
5439 CCGCGG
Eam1105I 1094 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2708 GACCGGATGTC
EcoO109I 1018 AGGACCT [GA]G^G[ACGT]CC[CT]
5139 GGGCCCT
EcoT22I 3456 ATGCAT ATGCA^T
4161 ATGCAT
FbaI 1158 TGATCA T^GATCA
5033 TGATCA
HaeII 221 AGCGCC [GA]GCGC^[CT]
574 AGCGCC
HhaI 222 GCGC GCG^C
575 GCGC
HinP1I 222 GCGC G^CGC
575 GCGC
NsiI 3456 ATGCAT ATGCA^T
4161 ATGCAT
PshBI 971 ATTAAT AT^TAAT
1828 ATTAAT
RcaI 414 TCATGA T^CATGA
1990 TCATGA
SacI 3123 GAGCTC GAGCT^C
4871 GAGCTC
SspI 4103 AATATT AAT^ATT
5590 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AciI AcsI AfaI AflII AluI
AlwI ApoI Asp700I AspHI AvaI
AvaII BalI BanII BbvI BcgI
BfaI BfrI BmyI BpmI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BsrDI BsrGI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
Cfr13I DdeI DpnII DraI EaeI
EarI EcoRI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RsaI SapI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmlI SspBI StyI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I XhoII XmnI